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EMAGE:16632

Slc5a6 solute carrier family 5 (sodium-dependent vitamin transporter), member 6 ( MGI:2660847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632
"Pseudo-wholemount" of euxassay_016837. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016837_01 euxassay_016837_02 euxassay_016837_03 euxassay_016837_04
EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632
euxassay_016837_05 euxassay_016837_06 euxassay_016837_07 euxassay_016837_08 euxassay_016837_09
EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632
euxassay_016837_10 euxassay_016837_11 euxassay_016837_12 euxassay_016837_13 euxassay_016837_14
EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632
euxassay_016837_15 euxassay_016837_16 euxassay_016837_17 euxassay_016837_18 euxassay_016837_19
EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632 EMAGE:16632
euxassay_016837_20 euxassay_016837_21 euxassay_016837_22 euxassay_016837_23 euxassay_016837_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16632Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16632_wholemount_strong.wlz
16632_wholemount_moderate.wlz
16632_wholemount_weak.wlz
16632_wholemount_possible.wlz
16632_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16632_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63307
Entity Detected:Slc5a6, solute carrier family 5 (sodium-dependent vitamin transporter), member 6 ( MGI:2660847)
Sequence:sense strand is shown

>T63307
TTTCCTCTCATCTGGGATCAGTGCTCCAGGCAGCACTCAGTATCTTTGGCATGGTTGGAGGGCCACTGCT
GGGACTCTTCTGCCTTGGACTGTTCTTTCCATGCGCCAACCCTCTTGGTGCCATTGTAGGCCTGTTGACG
GGACTCACCATGGCCTTCTGGATCGGCATTGGGAGCATAGTAAGCAGGATGAGTTCTGCTGTGGCACCCC
CTCCCCTTAATGGATCCAGCTCCTTCCTGCCTGCCAATGTAACTGTCGCCGCTGTGACCACCGTGATGCC
TTCTACCCTGTCCAAGCCCACAGGACTGCAGCACTTCTATTCCCTGTCCTATTTATGGTACAGCGCGCAC
AACTCCACCACAGTCATTGTAGTGGGCCTGATTGTCAGTCTGCTCACTGGGGGAATGCGGGGCCGGTCCC
TGAACCCCGGCACCATTTACCCTGTGCTGCCAAAGCTCCTAGCACTCCTGCCCCTATCCTGCCAGAAGCG
GCTTTGCTGGAGAAGCCACAGCCAGGATATCCCTGTGATCCCCAACCTGTTTCCAGAGAAGATGAGGAAT
GGGGTGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70544. Forward Primer - name:070544_F_cDNA_Slc5a6, sequence:TTTCCTCTCATCTGGGATCAGT; Reverse Primer - name:070544_N_SP6_cDNA_Slc5a6, sequence:AGCACCCCATTCCTCATCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16636 same embryo
 EMAGE:16631 same embryo
 EMAGE:16634 same embryo
 EMAGE:16633 same embryo
 EMAGE:16635 same embryo
 EurExpress:euxassay_016837 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS