Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16666

Ier5 immediate early response 5 ( MGI:1337072)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666
"Pseudo-wholemount" of euxassay_016864. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016864_01 euxassay_016864_02 euxassay_016864_03 euxassay_016864_04
EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666
euxassay_016864_05 euxassay_016864_06 euxassay_016864_07 euxassay_016864_08 euxassay_016864_09
EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666
euxassay_016864_10 euxassay_016864_11 euxassay_016864_12 euxassay_016864_13 euxassay_016864_14
EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666
euxassay_016864_15 euxassay_016864_16 euxassay_016864_17 euxassay_016864_18 euxassay_016864_19
EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666 EMAGE:16666
euxassay_016864_20 euxassay_016864_21 euxassay_016864_22 euxassay_016864_23 euxassay_016864_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16666Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16666_wholemount_strong.wlz
16666_wholemount_moderate.wlz
16666_wholemount_weak.wlz
16666_wholemount_possible.wlz
16666_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16666_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63268
Entity Detected:Ier5, immediate early response 5 ( MGI:1337072)
Sequence:sense strand is shown

>T63268
TCTAGCTTCTCGGGACTCCTACGGAAAAGCCCCGCAGGCGGCCGAGAGGAAGAGGAGGCGGAGGAGGGCG
GCCCGGAAGCCGCCGAGCCGGGGCAGATCTGCTGCGACAAGCCGGTGCTGAGAGACATGAGCCCCTGGAG
CACAGCCATCGTGGCCTTCTGAGCCCCCGGGACCCGAGTGGCTGGAGCTTGAGCAGCGGGAGTCCCCGAA
GTGCGGCCGGGGCCTCTCCCGGCCGCGAGGACGAGCCGAGACCGTAGGCGCTGGGCGCGTAGGGGAGACT
GGTTGCTGCCGGGCAGCACATCGCCCCAGAAGGGGCAGCCCGGCTGGGCCGTGGCTCTCGAGTCTCATCC
TGAGACCTAGAGTTGGAGGCTTATTGGAAGAGGACGATCGATCGTTAACTTTTGTATCTGTGTGTGTCCG
TGTGTAAGTTTGTCTTGTGTTGAGACCGTTCTGTGATTCTGGAATGCACCACCCCTTCCCCGGGGCTTGA
GACTTTGGGGGTGCAGACAGGGACTTTCCTCTGCCAGCAGCACAGAAAAGGAAGCTGCAGAAAGGCTCGA
GGAAGGGGAGTTCCCCCGTTCGATCTTGGGGGAGAGAGGGACTTTACACACACCTCTCTCTCATGAAAGC
TACG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141682. Forward Primer - name:141682_F_cDNA_Ier5, sequence:TCTAGCTTCTCGGGACTCCTAC; Reverse Primer - name:141682_N_SP6_cDNA_Ier5, sequence:CGTAGCTTTCATGAGAGAGAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16668 same embryo
 EMAGE:16665 same embryo
 EMAGE:16667 same embryo
 EMAGE:16669 same embryo
 EurExpress:euxassay_016864 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS