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EMAGE:16754

Mir125a microRNA 125a ( MGI:2676809)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754
"Pseudo-wholemount" of euxassay_019311. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019311_01 euxassay_019311_02 euxassay_019311_03 euxassay_019311_04
EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754
euxassay_019311_05 euxassay_019311_06 euxassay_019311_07 euxassay_019311_08 euxassay_019311_09
EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754
euxassay_019311_10 euxassay_019311_11 euxassay_019311_12 euxassay_019311_13 euxassay_019311_14
EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754 EMAGE:16754
euxassay_019311_15 euxassay_019311_16 euxassay_019311_17 euxassay_019311_18 euxassay_019311_19
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euxassay_019311_20 euxassay_019311_21 euxassay_019311_22 euxassay_019311_23 euxassay_019311_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16754Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16754_wholemount_strong.wlz
16754_wholemount_moderate.wlz
16754_wholemount_weak.wlz
16754_wholemount_possible.wlz
16754_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16754_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70247
Entity Detected:Mir125a, microRNA 125a ( MGI:2676809)
Sequence:sense strand is shown

>T70247
TCCCTGAGACCCTTTAACCTGTGA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-125a-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16753 same embryo
 EMAGE:16750 same embryo
 EMAGE:16751 same embryo
 EMAGE:16752 same embryo
 EurExpress:euxassay_019311 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS