EMAGE mouse gene expression spatial database

EMAGE:16772

Mir504 microRNA 504 ( MGI:3718543)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019320. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019320_01	euxassay_019320_02	euxassay_019320_03	euxassay_019320_04

euxassay_019320_05	euxassay_019320_06	euxassay_019320_07	euxassay_019320_08	euxassay_019320_09

euxassay_019320_10	euxassay_019320_11	euxassay_019320_12	euxassay_019320_13	euxassay_019320_14

euxassay_019320_15	euxassay_019320_16	euxassay_019320_17	euxassay_019320_18	euxassay_019320_19

euxassay_019320_20	euxassay_019320_21	euxassay_019320_22	euxassay_019320_23	euxassay_019320_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

weak

regional

weak expression: see section 09 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70075
Entity Detected:	Mir504, microRNA 504 ( MGI:3718543)
Sequence:	sense strand is shown >T70075 AGACCCTGGTCTGCACTCTATC
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-504 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16773 same embryo
	EurExpress:euxassay_019320 same experiment
	MGI:4826324 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source