EMAGE mouse gene expression spatial database

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EMAGE:16850

Zfp97 zinc finger protein 97 ( MGI:105921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008767. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008767_01	euxassay_008767_02	euxassay_008767_03	euxassay_008767_04

euxassay_008767_05	euxassay_008767_06	euxassay_008767_07	euxassay_008767_08	euxassay_008767_09

euxassay_008767_10	euxassay_008767_11	euxassay_008767_12	euxassay_008767_13	euxassay_008767_14

euxassay_008767_15	euxassay_008767_16	euxassay_008767_17	euxassay_008767_18	euxassay_008767_19

euxassay_008767_20	euxassay_008767_21	euxassay_008767_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16850_wholemount_strong.wlz

16850_wholemount_moderate.wlz

16850_wholemount_weak.wlz

16850_wholemount_possible.wlz

16850_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16850_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

moderate

regional

moderate expression: see section 11 12

rectum

moderate

regional

moderate expression: see section 11

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2833
Entity Detected:	Zfp97, zinc finger protein 97 ( MGI:105921)
Sequence:	sense strand is shown >T2833 TGGCCTCGAGGCCAGATTCGGCACGAGGACCATTCTGGAGTTGTTGACCGCCTGACATCCTCTGGTACGG GACAGGAAGGAGGGCGGTCGGCCGAGTGGGGTCCAGCGGGGCCAAGGCGAAGCTGAGCATCTCCTGGAGC TGCCCCCTGTAGAGGGGAGAGAGATGGCTGACCCTGAGAGCCCGTGGTCACAGATTGGCAGGAAGATCAA ACTGGAAGGACTGTCTGATGTTGCCTCTATTTCAACTAAACTTCAGAACACTCTGATCCAGTATCACAGC ATCGAAGAAGATGAGTGGCGAGTTGCCAAAAAAGCGAAAGATGTGACCGTTTGGAGAAAACCCTCAGAAG AGTTTAATGGGTATCTATATAAAGCTCAAGGAGTTATGGATGACGTGGTCAATAATGTAATAGACCATAT CCGCCCAGGGCCCTGGCGCTTGGACTGGGACCGGTTAATGACCTCCCTGGATGTTCTGGAGCACTTTGAA GAGAATTGTTGTGTGATGCGTTACACCACTGCTGGGCAGCTTTTAAATATTATTTCCCCGAGAGAGTTTG TTGATTTCTC
Notes:	The probe template was PCR amplified from IMAGE:1528796 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1528796 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16851 same assay
	EMAGE:16853 same embryo
	EMAGE:16852 same embryo
	EMAGE:16854 same embryo
	EurExpress:euxassay_008767 same experiment
	MGI:4828483 same experiment
	EMAGE:16855 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source