Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16941

Fam177a family with sequence similarity 177, member A ( MGI:1920635)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941
euxassay_008747_01 euxassay_008747_02 euxassay_008747_03 euxassay_008747_04 euxassay_008747_05
EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941
euxassay_008747_06 euxassay_008747_07 euxassay_008747_08 euxassay_008747_09 euxassay_008747_10
EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941
euxassay_008747_11 euxassay_008747_12 euxassay_008747_13 euxassay_008747_14 euxassay_008747_15
EMAGE:16941 EMAGE:16941 EMAGE:16941 EMAGE:16941
euxassay_008747_16 euxassay_008747_17 euxassay_008747_18 euxassay_008747_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16941Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16941_wholemount_strong.wlz
16941_wholemount_moderate.wlz
16941_wholemount_weak.wlz
16941_wholemount_possible.wlz
16941_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16941_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2836
Entity Detected:Fam177a, family with sequence similarity 177, member A ( MGI:1920635)
Sequence:sense strand is shown

>T2836
TCNAGCCAGATTCGGCACGAGGTTTTTTTTCATCTTGCTCCTTTTATTATACATTCTGAACCCTCCTTGT
GTAAACTCTAGGGGTACTTTATACCGAGGCTGTAGGATTATTAGTGATTAGTCAATACTTTCAAAAACAA
CTCCAAATGCCCCCAAACCAAAAATCTTAATGAATTATTTCACGCTCTTAATGTAGAATGCCTTCAGTTT
CTGTTGTTGTTTTAAGGCCAGGTGTCACCATGTGCTAGGCTGGAGCTCACTGTGTTGACCAGGGCGGCCG
TAAACTCACCTGGTGCAAAGGTTAAAGGCATGTAGTACCATGCTTGGCTAAATGTCCTTTGTGTTTTATA
AAAGTTCGTCTTAAAATCACAGCCCTGGGCTTAGGATCGAGTGCTTGGCTGGTGAGTGTATGTGAAAGGA
GCCCTGGGTTTGCTCCTTGGCATTGGGGGTGGGAAATGGGGGTCACTAACTCAAAAGCACCCCAAATTCA
GACCTCACTGTAATAGATACAGCATAAGAATGAAGGAAGGTTTTCCCGTAATAATTTAAAATATAAATAG
TTGTTTTAAGT
Notes:The probe template was PCR amplified from IMAGE:1528880 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1528880 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16942 same embryo
 EMAGE:16939 same embryo
 EMAGE:16938 same embryo
 EMAGE:16940 same embryo
 EurExpress:euxassay_008747 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS