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EMAGE:17036

Ube2l3 ubiquitin-conjugating enzyme E2L 3 ( MGI:109240)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036
euxassay_008847_01 euxassay_008847_02 euxassay_008847_03 euxassay_008847_04 euxassay_008847_05
EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036
euxassay_008847_06 euxassay_008847_07 euxassay_008847_08 euxassay_008847_09 euxassay_008847_10
EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036
euxassay_008847_11 euxassay_008847_12 euxassay_008847_13 euxassay_008847_14 euxassay_008847_15
EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036 EMAGE:17036
euxassay_008847_16 euxassay_008847_17 euxassay_008847_18 euxassay_008847_19 euxassay_008847_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17036Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17036_wholemount_strong.wlz
17036_wholemount_moderate.wlz
17036_wholemount_weak.wlz
17036_wholemount_possible.wlz
17036_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17036_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1293
Entity Detected:Ube2l3, ubiquitin-conjugating enzyme E2L 3 ( MGI:109240)
Sequence:sense strand is shown

>T1293
NNGATCCAGATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAGATCCGCAAATGTGGAATGAAAA
ACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCC
TCCATATGATAAGGGAGCCTTCAGAATTGAAATCAACTTTCCAGCAGAGTATCCATTCAAACCACCCAAG
ATCACATTTAAAACAAAGATCTACCACCCTAACATCGATGAGAAGGGGCAGGTCTGTCTGCCAGTCATTA
GTGCTGAAAACTGGAAGCCAGCCACCAAGACTGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGA
CCCCCAGCCTGAGCACCCACTCCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGT
AAGAATGCTGAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAAAATCTGCCATGAGTGA
TTCCAGCAAGTTTGAGCAGAGACCCCGAGCAGTGCATTCAGAC
Notes:The probe template was PCR amplified from IMAGE:2236301 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2236301 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17033 same embryo
 EMAGE:17031 same embryo
 EMAGE:17034 same embryo
 EMAGE:17035 same embryo
 EMAGE:17032 same embryo
 EurExpress:euxassay_008847 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS