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EMAGE:17051

Fchsd2 FCH and double SH3 domains 2 ( MGI:2448475)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051
"Pseudo-wholemount" of euxassay_001442. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001442_01 euxassay_001442_02 euxassay_001442_03 euxassay_001442_04
EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051
euxassay_001442_05 euxassay_001442_06 euxassay_001442_07 euxassay_001442_08 euxassay_001442_09
EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051
euxassay_001442_10 euxassay_001442_11 euxassay_001442_12 euxassay_001442_13 euxassay_001442_14
EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051 EMAGE:17051
euxassay_001442_15 euxassay_001442_16 euxassay_001442_17 euxassay_001442_18 euxassay_001442_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17051Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17051_wholemount_strong.wlz
17051_wholemount_moderate.wlz
17051_wholemount_weak.wlz
17051_wholemount_possible.wlz
17051_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17051_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 16 17 18 19
tail dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3228
Entity Detected:Fchsd2, FCH and double SH3 domains 2 ( MGI:2448475)
Sequence:sense strand is shown

>T3228
TCTACTGGAGAGGCCCTTATATTATTTTATTCCCAAGCCAGAGTATTGGCCTAGACATCTGGGTTGTGAT
TGGTGCAGACCAGCTGTCGCCTTCTGAAAGTACAGCTTCAGGTCCCACTGCCCTGACATTTGTCCAGAGT
GAAGAATCTATGGGCCCTGGAGGAAGCCTGCTTTCTGTGGAAAGGATACAGGACTGTACACCTGCCCAGG
TGAGACCAAGGAGGCTAGCACCTTCTGCAGGTCAGGGAAGAGCCTCATGGCGCCCTGGCCTCAGCCTAGA
AGGTCAAGCACTCTGTGGACGGTAGATCTTGACCTAGGGAACAAAGCAATGCTTGCCAATCCTCTTTTAC
CTTGGCTGGGAAAAGAAATTTACAGATTTAAAGGTTGTATGATGCTTTGAAATTTCCATCCCTTCTGAGA
ATCTCTAAAAACTTGAAATGGTCACATGTCCCCGTGGGATTTTTGACTGATATCGGAGCTGAAGGGATTT
TCAGTCATTTGATCACTTTAGCAATGA
Notes:The probe template was PCR amplified from IMAGE:2749129 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2749129 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17048 same embryo
 EMAGE:17049 same embryo
 EMAGE:17047 same embryo
 EMAGE:17050 same embryo
 EMAGE:17046 same embryo
 EurExpress:euxassay_001442 same experiment
 MGI:4824819 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS