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EMAGE:17061

Pde8a phosphodiesterase 8A ( MGI:1277116)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061
"Pseudo-wholemount" of euxassay_001451. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001451_01 euxassay_001451_02 euxassay_001451_03 euxassay_001451_04
EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061
euxassay_001451_05 euxassay_001451_06 euxassay_001451_07 euxassay_001451_08 euxassay_001451_09
EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061
euxassay_001451_10 euxassay_001451_11 euxassay_001451_12 euxassay_001451_13 euxassay_001451_14
EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061 EMAGE:17061
euxassay_001451_15 euxassay_001451_16 euxassay_001451_17 euxassay_001451_18 euxassay_001451_19
EMAGE:17061
euxassay_001451_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17061Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17061_wholemount_strong.wlz
17061_wholemount_moderate.wlz
17061_wholemount_weak.wlz
17061_wholemount_possible.wlz
17061_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17061_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 18 20
cranium
moderate moderate
regionalmoderate expression: see section 04 05 06 16 17 18 20
pectoral girdle and thoracic body wall skeleton
moderate moderate
regionalmoderate expression: see section 08 09 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3241
Entity Detected:Pde8a, phosphodiesterase 8A ( MGI:1277116)
Sequence:sense strand is shown

>T3241
TGGCCTCGAGCCAGATTCGGCACGAGGGTANACTCCTTGCCATTCCTTGTCTACTGTGACACACGAATTT
ATGGCATCGAGCAAATCACCTTTCTTTTCATTTATGGGCACTTGTGCTAACTCCTTCCCTATTAATTCTC
CTGACTGATAACCCATTGTTGATTCAAATGCAGGGTTTGCATACTGTATAATGTGGTCTTCACTTGTAAT
TTCAATTGCTTCTTGACTTTTCTCTAATGCAGTGAATACTGAGTTACAAGCCCTGAGTTTCAGCTGTGAT
CGCACCTCTCCACATGCCAACTGGAGCAGTTCATTATAACAGGCCATGACGTTGGGGTTCTCTATAAACC
TCCGTGTGAAGCCAGCAGCAAGGAAGGGCATTAAAGATGATTCTTCTTTGTCCACCCTGCGTACTACGCC
AACAATAACTGTGTTTTCTGAGAATTTTGATGATCTGATAGACCTGCACAATGTCTCTGCATCCATCTGT
CGGGGATATCTGTGGTCTATGATGATGATGTCATGGAGTTTGTCCTGGAAACAGGTAAGGA
Notes:The probe template was PCR amplified from IMAGE:303522 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:303522 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17059 same embryo
 EMAGE:17058 same embryo
 EMAGE:17060 same embryo
 EurExpress:euxassay_001451 same experiment
 MGI:4827122 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS