EMAGE mouse gene expression spatial database

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EMAGE:17114

Adamts16 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 16 ( MGI:2429637)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001489. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001489_01	euxassay_001489_02	euxassay_001489_03	euxassay_001489_04

euxassay_001489_05	euxassay_001489_06	euxassay_001489_07	euxassay_001489_08	euxassay_001489_09

euxassay_001489_10	euxassay_001489_11	euxassay_001489_12	euxassay_001489_13	euxassay_001489_14

euxassay_001489_15	euxassay_001489_16	euxassay_001489_17	euxassay_001489_18	euxassay_001489_19

euxassay_001489_20	euxassay_001489_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17114_wholemount_strong.wlz

17114_wholemount_moderate.wlz

17114_wholemount_weak.wlz

17114_wholemount_possible.wlz

17114_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17114_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 21

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

pons ventricular layer

strong

homogeneous

strong expression: see section 12

midbrain ventricular layer

strong

regional

strong expression: see section 12

dorsal grey horn

moderate

regional

moderate expression: see section 08 09 10 13

ventral grey horn

moderate

regional

moderate expression: see section 09 10 11 12 13

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12

kidney calyx

strong

regional

strong expression: see section 03 05 06 07 08 09 14 15 16 17 18

testis

strong

regional

strong expression: see section 04 05 06 07 15 16 17

tail mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4491
Entity Detected:	Adamts16, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 16 ( MGI:2429637)
Sequence:	sense strand is shown >T4491 CGGACGCGTGGGCTCCTTCTCTACCCTCCTCCCTGCTGTCCCTTGGTTGCTGCGGGTGCTTTGCCGCCCC CCGCCACCACTGCCACTCCACCTCCCGGTGCCGAACGAAGCTGCAGTGATAACTCTGATGTGCATGGTGG AATCGCTGCCTGGAAACCCTGCGATTTTGCCGGGTCGGTCCTTTGCACCCTAGCATACTGCTAACACCGG GACTATCCAGTGACCCAGTCTAGACTGCTGAGCTGAGTCCTGTGAGCCCTCGGGCACCGGGGCCATGGAG TCCCGAGGTTGTGCTGCGCTCTGGGTGCTGCTGCTGGCGCAGGTCAGTGAGCAGCAGACACCTGCGTGCG CCCTGGGACTCGCAGCTGCAGCGTCTGGGAGCCCGGAAGACCCGCAGCCTCCCCCATTCTCTGGGTCCAG CTGGCTGGAAACCGGTGAGTATGACCTGGTGTCTGCCTATGAAGTAGACCACAGGGGGGATTATGTATCC CATGACATCATGCACTACCAGCGGAGGCGTCGGAGAAGAGCTGTGACCCAGCCAGGAGGAGATGCCCTGC ACCTAAGGCTTAAGGGCCCTAGGCATGACCTCCACCTCGACCTCAAAGCTGCCAGCAACTTGATGGCTCC TGGATTCATGGTGCAAACTCTGGGGAAGGGAGGCACCAAGTCTGTACAGATGTTTCCACCAGAAGAGAAC TGCTTTTACCAAGGATCCTTAAGGTCCCAGGGGAATTCCTCAGTGGCCCTTTCTACCTGCCAAGGTTTGT TAGGCATGATAAGAACAAAAGACACTGATTACTTCTTGAAGCCACTGCCTCCACACCTAACAAGCAAACT GAACAGATCTGCACAGGGCGACTCCCCCTCCCATGTTCTGTACAAGAGATCTACAGAGCGCCAGGCTCCT AGAGAAAACGAAGTCCTGATGATCACCAGGAAGCGAGATCTGGCAAGACCACACCTGCACCATGACAACT TCCATCTTGGCCCCTCACAAAAGCAGCATTTCTGTGGAAGACGCAAGAAATACATGCCTCAGCCTCCCAA CGATGACCTCTACATCTTGCCTGATGAGTACAAGCCTAGTTCCCGACACAAGCGCTCGCTCTTGAAATCC CACAGAAATGAAGAGTTAAATGTGGAGACACTGGTGGTGGTTGACAGAAAGATGATGCAAAGCCATGGCC ATGAAAACATTACGACTTATGTCCTCACCATTCTCAACATGGTGTCTGCCTTATTCAAGGATGGAACAAT TGGGGGAAACATCAACATTGTCATTGTGGGACTCATTCTGCTGGAAGATGAACAGCCAGGGCTGGCGATT AGTCACCATGCAGACCACACGTTAACCAGCTTCTGCCAGTGGCAATCCGGATTGATGGGGAAAGATGGAA CTCGTCATGACCACGCCATCTTACTGACTGGTCTGGACATATGTTCCTGGAAGAATGAGCCCTGTGACAC ATTGGGGTTTGCACCCATAAGCGGGATGTGCAGTAAGTACCGCAGCTGCACAGTCAATGAAGACTCAGGA CTTGGACTGGCCTTCACCATTGCCCATGAGTCTGGACACAACTTTGGCATGGTCCATGATGGAGAAGGGA ATATGTGCAAGAAATCTGAGGGCAACATTATGTCCCCAACACTGGCAGGACGCAATGGTGTCTTCTCCTG GTCTTCCTGCAGCCGTCAGTACCTGCACAAGTTCTTAAGCACCGCCCAAGCGATATGTCTTGCTGATCAG CCAAAGCCTGTGAAAGAGTATAAGTACCCCGAGAAGCTGCCGGGACAGTTATACGATGCAAATACCCAAT GCAAGTGGCAGTTTGGAGAGAAAGCCAAGCTCTGTATGCTGGACTTCAGAAAGGACATCTGTAAGGCCTT GTGGTGCCATCGGATTGGAAGGAAATGTGAGACCAAGTTCATGCCAGCAGCAGAGGGTACTCTGTGTGGG CAGGACATGTGGTGTCGTGGAGGACAGTGTGTCAAGTACGGTGATGAAGGCCCAAAGCCCACCCATGGCC ATTGGTCAGATTGGTCCCCCTGGTCCCCCTGCTCCAGGACCTGTGGGGGAGGAATCTCTCACAGAGACCG TCTCTGTACCAATCCCAGACCATCTCATGGAGGGAAGTTTTGCCAGGGCTCCACACGCACTCTGAAGCTT TGCAACAGTCAGAGATGCCCTCTGGACAGTGTGGATTTCCGTGCTGCCCAGTGTGCCGAGTATAACAGCA AGCGCTTTCGAGGGTGGCTCTACAAGTGGAAGCCTTACACCCAGTTGGAAGATCAGGACTTATGCAAACT CTACTGTATCGCAGAAGGATTTGATTTCTTCTTTTCTTTGTCAAATAAAGTGAAAGATGGGACTCCATGC TCGGAGGATAGCCGTAATGTTTGTATAGATGGGATGTGTGAGAGGGTTGGCTGTGACAATGTCCTTGGAT CAGATGCAACAGAGGACTCCTGTGGGGTCTGCAAGGGGAATAACTCAGACTGTGTCACGCATAGAGGGCT CTACAGTAAGCACCATTCCACCAACCAGTACTACCACATGGTAACCATTCCTTCTGGAGCTCGGAGCATT CACATCTACGAAACAAACATCTCTACCTCCTATATTTCTGTGCGCAATTCTCTCAAGAGATATTACCTGA ATGGACACTGGAGTGTGGACTGGCCTGGGCGATACAAGTTTTCGGGTGCCACCTTCAACTACAAACGGTC CTACAAGGAGCCTGAGAATTTAACCTCCCCTGGACCAACCAATGAAACTCTGATTGTGGAGCTTTTGTTC CAAGGCAGGAACCCTGGTGTGGCCTGGGAATTTTCATTGCCAAGGTCAGGGGCTAAGAAGACTCCAGCTG CCCAGCCCAGCTACTCATGGGCCATCGTACGCTCTGAGTGCTCCGTCTCCTGTGGAGGGGATAGGCAGTG CCAAACTGGGAGAGGACATTTGGAAATCTCTTCATAAATTCTGCCTGGCACCAGGAGGAGGTGTCCTCCT TCTGGGATTATGAACAGCCATGTTGCCTTTTGTGATGGGCCAGATTCCATAGGGAAATGATGTCCTGGCA GCCGTGAGCAACCACTGCCCTCCACCTTCCTGTCTGGCTACTTTGTTTACTTCTTAATCTCCATTTTTTA TTTATGACATAACCAGACTTTAGTTCAATTTTCCTGCTCTGCCAGGCCTTGAATTGACATTCATTCACCC CCTGGGATATGGCAGGGGAAGCCAAGAATCTTGTGGTGATCCAGCTGATCTGGTGGTGTCTGCTCTCTGG AAGGGAGACTTAAATTTGGTGCTGAGCTCGTGTTTGCTGTTCACTCCATAAAAGCATCAGATTTTCTTCA AGTTTTTATAAAAATGTAATTTTTTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4951547 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4951547 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17112 same embryo
	EMAGE:17113 same embryo
	EMAGE:17115 same embryo
	EMAGE:17116 same embryo
	EurExpress:euxassay_001489 same experiment
	MGI:4822985 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source