EMAGE mouse gene expression spatial database

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EMAGE:17129

Lad1 ladinin ( MGI:109343)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001510. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001510_01	euxassay_001510_02	euxassay_001510_03	euxassay_001510_04

euxassay_001510_05	euxassay_001510_06	euxassay_001510_07	euxassay_001510_08	euxassay_001510_09

euxassay_001510_10	euxassay_001510_11	euxassay_001510_12	euxassay_001510_13	euxassay_001510_14

euxassay_001510_15	euxassay_001510_16	euxassay_001510_17	euxassay_001510_18	euxassay_001510_19

euxassay_001510_20	euxassay_001510_21	euxassay_001510_22	euxassay_001510_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17129_wholemount_strong.wlz

17129_wholemount_moderate.wlz

17129_wholemount_weak.wlz

17129_wholemount_possible.wlz

17129_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17129_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 16 17 18 19

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 17

spinal cord

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 14 15

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11

neural retina

weak

regional

weak expression: see section 01 02 03 04 05 22 23

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

renal cortex

moderate

regional

moderate expression: see section 13 14 15 16

tail dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4517
Entity Detected:	Lad1, ladinin ( MGI:109343)
Sequence:	sense strand is shown >T4517 CCCACGCGTCCGGCGCGGGAGACCCGCCCCAGCGGTGATCAGCGCGGCTTTGTCTGGGACGCCCACACCT CAGAGGCCGCTCCCCCGGCCGGCGAATGGGAGCGCGGCGGCGCGGGGCCGAGCCGGGCTGAGATAAGCGG CCATGTGACCCTACCTGGCGCGGGGAGGGGCCGCAGGTGAGCGGCGGAGCAGGGAGCTCGGCACGCGGCG GCCGTGGGGCTCGGCAGCATGTCGGTCAGCAGAAAGGACTGGTCCGCGCTGTCCAGCCTTGCCCGACAGC GGACTTTGGAGGACGAAGAGGAGCAGGAGCGGGAACGCAGGCGGAGGCACCGCAACCTGAGTTCAACCAC AGATGATGAATCTCCAAAACTCACCCAGAATGGAGCTCAGAGATCTGTGGAGAGGCTGCCCAGTGTGGAG GAAGCAGAGGTCTCTAAGCCATCGCCCCCAGCCTCCAAAGATGAAGATGAAGACTTCCAGGCCATCCTTA GGACCAGAAAGGAGCGGAGACAGAGGCGGCAGGTGGTAGAGGCTGTCCAGGCTCCAGTACAGGAGAGGCC AGAGGCAGAGGAGGAAAGAGACAGCCTGGGCCCTGAGCAGACCTCGTCACAGCCCCTTGTGCCTAAGAAG AAAGTGGAGGCCCTGCCTCGCCGGAGACTGAGTCGGGAGCAGAGAGGCCCTTGGGCCCAGGACGAAGAAC GCCTAAAGAACAGAGAGCTTGCGGAAGGAGAAAAGAGGCTTCCAGAAGAGACGGTGGCTCAGCAGAAGAC CTTGGTCTCTGAGAAAACGCCTGTCTCAGAGAAGACCCCAGTGCCTGCCAAAAGACTGGTTTCTGAGAAA GCCTGCCCCTCAGAGAAGGGGACAGCCACAGAGAAAGCGTCTCTGACTGAGAAAAGACATAGCCCAGAGA AGTTGGTTCCAGAAAAAACAAGTGTGACAGAGAAGTCACCGGTCCCTGAGAAGACGCTTGTGTCTCTGAA GACGGCAGCACCAGAGAGAAGGTCCCCTCCAGTTCTAGAAAAAGCCATCGTCTCAGAGAAGATGCAGGAG AGGAAGCTGGTGTCGGAGAAAGCATCCATCTTTGAGAAGTCACTAGTCTCAGAGGCAAAGCTGACGCCAA AGAAGGCAGCAGTGTCAGAGCAGCCCCAGACCACTGGGGGAAGCCAGGCCACCACGAGGGAGCCCAGAGG AAGGGCCCTCCCTGACAAGAGCCCACCTTCTTCTGCAGAGCAGAGCACACCAGCCCCGCCGACCAAAGCC TCTCGTTTCCCACCCATCACACTCCAGGTGAAAATTCCCAGCAAGGATGAAGATGCAGACACACCTTCAC CCACCCTGCTCACCTACAGCAGTTCCCTCAAACGCTCCAGCCCCAGGACCATCTCCTTCCGGATGAGCCC CAGGAAAGACAACTCAGAAACACCCTTAACCCGCAGTGCCAGCGTGAGGCTGCCGGCTAGCACCGTCAAG CTAGGGGAGAAACTGGAGAGATACCACACAGCCATCCAGAGGTCAGAATCCGTCAGGTCTCCAGGCTCCT CCCGTACTGAAGTCCTTGTCACACCTGCCGGTGTAGCCAGCAAGCGACACCTCTTTGAGAAGGAGCTGTC AGGCCAGAACCGCACAGAACCAACCTCCATCCGAAAGGAGAATCTGCGTCTGTCAGGGGTCGTGACATCA AGACTTAACCTGTGGATCAGCAAGACCCAGGATTCGGGAGACCACGGTTCTCAGGAGGTACGGAAAGAGG CATCAGTCACCAAGAGGGCTCAATGGGGAAGCAAGCCGTCCACATCCCTGGATGCAGAGGTGTGACAAGT CCTGTCAGGACGGACCTGAAAACCTGCATCCCAAGGGCCCTTCATCATGCCGGCCCCTCTCCTGCAGAAC CCTTGACTCTTGCAGACCCCACTCCCTTCCCGCCTCTGGATCAGTCTGGTCAGGGTCCTCAGGGGCCAGG AACATGACTGCCACGCCTTCAGCCCGTCTCCAGCCCAGACTGTGGGCAGGTGTTCTCTGGAACTTTCTTG TCCCGCTGAAGGTACAATGTATCCTGAACCCGGAAAGTGCCAGAGCACAGCCCTGGCCCGTCAGCCATGG AGTGTCCATGTCCTCTCTAGGGACTGCCTCCCTTTCTCCTACTCACCTCCACCATCAAGCTGGGTTATTT TGCCAACTGCAGAGTTACATGTCTAGCCTGCTCCCAGCAGGCACTCGGTGTGCCTGTGATATCTCCACAC TGAGTGCTAGACCTATTTACCGCTCCCTGAGGCTTGTTCAAGCCAGACTGGAAAGGAGTCGCCAGGCTGC CTATGTGCTACACCCCACTGCTTGCACTGTGTTCCCCACCCTCCTGCTGCCCTGATCGGGAACTCGGTGC CTTTTCGTCTTACCACATGCCTTACTCTCAGCCAGAAAAACCTTTCCCAGGAAGGATCTGATAAACTCAT TCAGTCTCAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4954891 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4954891 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17131 same embryo
	EMAGE:17130 same embryo
	EMAGE:17128 same embryo
	EMAGE:17127 same embryo
	EurExpress:euxassay_001510 same experiment
	MGI:4825855 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source