EMAGE mouse gene expression spatial database

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EMAGE:17144

BC035947 cDNA sequence BC035947 ( MGI:2652858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001531. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001531_01	euxassay_001531_02	euxassay_001531_03	euxassay_001531_04

euxassay_001531_05	euxassay_001531_06	euxassay_001531_07	euxassay_001531_08	euxassay_001531_09

euxassay_001531_10	euxassay_001531_11	euxassay_001531_12	euxassay_001531_13	euxassay_001531_14

euxassay_001531_15	euxassay_001531_16	euxassay_001531_17	euxassay_001531_18	euxassay_001531_19

euxassay_001531_20	euxassay_001531_21	euxassay_001531_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17144_wholemount_strong.wlz

17144_wholemount_moderate.wlz

17144_wholemount_weak.wlz

17144_wholemount_possible.wlz

17144_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17144_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4537
Entity Detected:	BC035947, cDNA sequence BC035947 ( MGI:2652858)
Sequence:	sense strand is shown >T4537 AGAAACCGGAACTTGACCAGACCTCACAGATCAGCCGTGGCCGGGAGCAGCAACCTGGTTCACCGACGGG AGTAGTTTCATGGTAGAAGTCTTTATAGATACCTTTTCAGGGTGGGTTGAAGCATTCCCCACCAAGAAAG AAACAGCTACCATAGTAGCCAAGAAGATACTTGAAGAAATTCTTCCTCACTTCGGAGTACCCAAGGTAAT CGGGTCAGACAATGGGCATGCCTTCGTCTCTCAGGTGCCGCACCAGTTTGAAGTGGGAGACGCTGTCCTG GTGAGGAGACATCGAGCGGGGGAACCCGAGCCACGGTGGAAAGGACTCTACCTGGTGTTGCTGACAACAT CCACTGCCGTCAAAGTAGAAGGAATCTCTGCCTGGGTTCATGCGTCTTGTCCCACGTCAAGAAGGCTCCC CCAGAGGCTGATCCAGATGAGCGGGCCCTGGAAAGGACTACTAATCCTCTTAAGCTGCGCCTGCTGCACA GGGGTGGCTCCACCAGACTTTAACCCTCACACTCCAATTCAGCAGATTTGGGAGGTGCTCAATGAAGAGG GAAACATTGTGTGGGCGACCACCGCAGTGCACCCTCCTTGGACTTGGTGGCCTGATCTCACACCTGACAT CTGTAAGTTAGCGGCAGGATCTCTTACCTGGGACCTCCTGGATCATACCGATCTTAGTGACCCACCCCCT GAAGAGCGGTGTGTCCCAAACGGGATAGGGAGCACATATGGGTGTTCGGGGCAGTTCTACCGAGCTAATC TTAGAGCCGCAGAATTCTATGTTTGCCCTGGTCAGGGTCAGAGCAGAAGGCTTCGACAAAAGTGTGGAGG GGCATCAAGGTTTTTTTGCGGTAAATGGGGATGTGAAACAACAGGACATGCCTTCTGGAACCCTTCCTCT GCCTGGGACCTAATCACGGTAAAACGGGGTAGTGACCATGATGGGTCAAACCAAGGAGAAAGGGACTCCT CTAAGTATCCGGAGAGTGGGTGTGCTCATAAAAACAGCCCCTCAGGACCATGCAAAGGTAAATACTGCAA CCCCCTACTTATAAAATTCACCGAGAAAGGGAGACAAGATCGTCAGAGTTGGCTTAAGGGAAATAGGTGG GGTTGGCGAGTATATGCTCCATCAAGAGACCCTGGGTTCATTTTTAAGATCAGACTGACAGTGGGAGACC CGGCAGTTGCACCCATAGGGCCCAACACGGTCCTTCTGGAACAGGGCCCCCCAGCCAAATCCAAATCCCT GACTCAAATGCCCGCCCAGCCCATGGGCCCCTCTTATACTGACACTCTTACTCCCACCACCGCCTTACAA AACCCTCTGACTGTGGCCCCAACAAACCCTTCGACGGGACAGCGAATGTTTAACTTAGTGAGAGGAGCCT TCTATGCCCTCAACAGAACAAATCCAGATGCTACAGAGGACTGTTGGCTATGCTTGTCCTCGGGCCCCCC TTATTATGAAGGAATTGCCTTTAATGGAGATTTCAATAAAACTAGCAGCCATACCTCCTGCTCATGGGGG ACAGGGCAGAAACTGACCCTGACTAAGGTATCAGCAAGAAACCCAGGTCTCTGTATAGGTACTCCCCCTC CTACCCACAAACATTTGTGTGCCCAAATTCAGTCTGTATCCAAAACAGAAACTAATTATTACCTTGTGCC GTCTCCAGTTGGCTGGTGGGCCTGTAACACAGGGCTCACCCCCTGTGTATCAACTAAGGTTTTTGAATCT TCCCATGATTTCTGTGTTATGATCCAACTGTTACCTCGTGTGTATTATCATTCTGCATCTAGTCTAGAAG AAATATACGCTGGTACAAGATTTAAGAAAGAACCTGTTACTTTAACCTTAGCCACATTTTTGGGCATTGG GATGGCAGTAGGAGTGGGAACAGGAGTATCGGCCTTAATTGAAGGAAGACAAGGGATCCAGTCCTTAAGA GATGCTGTTAATGTAGACCTAGAGATGTTAGAAAAATCTACTGATGTGTTAGAAAAATCTTTGTCCTCAC TATCAGAAGTAGTACTACAAAACAGAAGGGGCCTCGATCTGCTCTTCCTAAAAGAAGGAGGACTGTGCAC AGCCCTAAAAGAAGAATGCTGTTTTTATGCAGATCACACTGGGATAGTCAGGGATTCTATGCAAAAGCGA GAGAAAGACTCGAACGACAAAAGCGAGAACGAGAGGCCCAACAGGGATGGTTAGAGTCGTGGTATTCTCG GTCACTGTGGACGACATCTTTATTTTCTGCCGTAGCTGGACCAGTTATTTTCATATGTTTAATTTTGATC TTTTGTCCCTGCATAATAAATAGAGGAATGGCTTTTATCCAGAGTAGGATAGATGCAGTCAAACTCCAGG TTTTACAATGACAATATCAACCCATAATTCAGATAGAAGAAGAATTAGGAGACACAGGCTTTTAAAATTC TACGATTAGAATTATTTAGTAGAAGAAGAGGGGAATGAAGGAAGATGAAATATTGTAACTTGTGAGTTGT CTAGGAGCCCACTGCCCCAGCCTGGGGCTGAGAACAAAGCAGAACAGGCCCAGGCATGCCAGGGCAGGGC TGTTGTTAAGGCATTCCTAAGAACATCTTGACTGTAAAGATGGAAAGGTATGCAAGGACAAGCAGGGCTG TCTTGTCTGAGTCAACACCATCTGGCCAGAACCTCCCCTCTGTCTATTACCCCTTGGCACCAGGTAAAGT CATACATCAACTGGTTGTTATGTAAACAAAGATAAGCCCCAAGCCCACCGGAACAAAGTCCTGATGCCCT TTTTGCTGACATGTAATCTTTCTGTTAATGTTTGAATGAGCCAATCCTGTACCTTTGTCGAAATTCCTCG CACCCCTAACCCTTTGCTTCTTAAAAAACCCCTAGCTTCCGAGCCTGGGGGTCGACTCCCCTGCCTCCTG TGTGAGGTATGTGTTGGCCCAGAGCTCTGCCAATAAAGCCTCGTGCGTCTTGCATCAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4975064 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4975064 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17143 same embryo
	EurExpress:euxassay_001531 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source