EMAGE mouse gene expression spatial database

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EMAGE:17147

Slc16a4 solute carrier family 16 (monocarboxylic acid transporters), member 4 ( MGI:2385183)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001537_01	euxassay_001537_02	euxassay_001537_03	euxassay_001537_04

euxassay_001537_05	euxassay_001537_06	euxassay_001537_07	euxassay_001537_08	euxassay_001537_09

euxassay_001537_10	euxassay_001537_11	euxassay_001537_12	euxassay_001537_13	euxassay_001537_14

euxassay_001537_15	euxassay_001537_16	euxassay_001537_17	euxassay_001537_18	euxassay_001537_19

euxassay_001537_20	euxassay_001537_21	euxassay_001537_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17147_wholemount_strong.wlz

17147_wholemount_moderate.wlz

17147_wholemount_weak.wlz

17147_wholemount_possible.wlz

17147_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17147_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 06 07

lateral ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 09 12 14 15 16 17 18

otic capsule

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22

exoccipital bone

moderate

regional

moderate expression: see section 04 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 04 05 06 18 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 07 08 09 10 11 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4538
Entity Detected:	Slc16a4, solute carrier family 16 (monocarboxylic acid transporters), member 4 ( MGI:2385183)
Sequence:	sense strand is shown >T4538 CTACAGTGCCTGGTTGTTTCCACCAAGCAGCGGAATGGGAAGCCTTAGAAAGAGAGTAACGTTTTAGGGA AAATAATTTTTCACCTGAAATTTTCTTGTCTCTAGAGGATTCATTTGCTTAAAGTGATCTCCGCATGTAG CCTACAGGAAGCATCATGGTGAAGAAGGAAAAAACGCCCTCCCCTTACACCAAACCCCTTGATGGAGGAT GGGGGTGGATGGTCGTGCTTCATTTTTTCCTGGTAAATGTGTTTGTGATGGGAATGACCAAGACATTTGC AATTTTCTTTGTGGTTTTTCAAGAAGAGTTTGAAGGCACTTCAGAGCAAATTGGTTGGATTGGATCCATT ATGTCATCACTCCGTTTCTCTGCAGGTCCCCTGGCTGCTATCATCTGTGACGTACTTGGAGAGAAAGCTA CCTCCATTCTTGGGACGTTCCTTGTCTCTGGTGGCTATGTCATCAGCAGCTGGGCCACAGGCATTCCCTT TCTTTGTGTGACCATGGGGCTGCTACCTGGTTTGGGATCTGCTTTCTTGTACCAAGTAGCTGCTGTGGTA ATTACCAAATATTTCAAAAAACGATTGGGCCTTTCTACAGCAATTGCCCGTTCTGGAATGGGGCTGACAT TTTTGTTGGCACCCTTTACAAAATTCCTCATAGATCTTTATGACTGGACAGGCGCTCTTATTTTATTTGG AGCAATTATATTGAACTTGGTGCCTTCCAGCATGCTCTTAAGACCCATCCACAGCCAATCAAACAACAAT TCTGACATTGAAAACAAAGGTAGCATTTTGTCTGCAACTGAACCAGAGGCATCATACAAGACAGAGACAT CGAAGTGCAAGGAGACACAGGAGCCTTTCATCAAAGACAGTACTATGAAGAAATCTGAACAACCTACCAC AACCTTAACTGTCTTAGGAAATCAAAGCGAAGAGTTCAGCAATAGGCCTCACAGGAACAGGCCGTTACTT ATGAGCAATGGCAAAAGTCACAAGAAAAAGTTTGTTTCATGGAACTGCAAACAAAAACTTTTAGATATCT CGCTCTTTAGAAACCCTTTCTTCTACATTTTTACCTGGTCTTTTCTCCTGAGCCAATTAGCATATTTCAT TCCCACTTTCCACCTAGTAGCCAGAGCAAAAACACTTGGGATTGACGTAATGGATGCCTCCTACCTTGTG TCTGTAGCTGGTATCACTGAGACAGTCAGTCAGCTCATTTCTGGATGGATTGCTGATCAAAATTGGATCA AGAAGTATCAATACCACAAGTCTTACCTCATTCTGTGTGGTGTGACTAACCTGCTTGCTCCTTTAGCCAC CACATTCCCACTCCTTATGGCCTATACCATCCTTTTTGCCATTTTTGCTGGTGGCTACCTGGCACTAATA CTGCCAGTATTGGTTGACCTATCTAAGAATTCTAGAGTACACAAGTTTTTGGGATATGCCAGTTTCTTTG CCGGGATAGCTGTACTTTCTGGACCACCAATAGCAGGCTGGATATATGATTACACACAGACTTACACAGG CTCTTTCTATTTCTCTGGCACCTGCTATATCCTCTCTTCAGTTTCCCTTTTTTTTGTACCACTGGCTGAA AGATGGAAACGCAAACAGTCTGACCTTCTGAGGACTACAATCAAGTAAGAGCCTAACAAACAAAAATCTA AACAAGCTACTGTAAGCAAAAGCTCAGAAGAAAAGTCTTTGTTAAGAAAGCAAGCCATAGAGTTTTTAAG ATTCCTTCCTACTTTCTTAGCACTGCCAGGAATGTCAACTGGTGGAAGGAGTAATAAATGAGTAATAAAG GAGCAACAACTTAAATGTAATCAAACAGGCTACAAACTGAATCCATTGAAGCAGCTCCTTTACTATTAAA TGAAAATCAGAATGTACTGTAATAAATCAATCCAATTTTTCATTTGTGCTATGGTAATTCTGTCACCAAA ATATGTAACAATTATAAACTATTTTTTTCTTTTATTTAAATATAGTCATTGGTTAACTAGATCTCTGGAA TCTGAATAAAGCCTTATTTAGTGACAAGAATAATACCCTCTCCAAAAAAGTTTATTGTTGTTTATTCATT ATAAGTAATCTTGTTTTCTTATTACTTTGTCTTTTAGATTTCTATAGTGTACTGCTATCTAATAAAAATA AAATTTGCTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4975010 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4975010 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17146 same embryo
	EMAGE:17148 same embryo
	EMAGE:17145 same embryo
	EurExpress:euxassay_001537 same experiment
	MGI:4828113 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source