EMAGE mouse gene expression spatial database

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EMAGE:17178

3632451O06Rik RIKEN cDNA 3632451O06 gene ( MGI:1914669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001572. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001572_01	euxassay_001572_02	euxassay_001572_03	euxassay_001572_04

euxassay_001572_05	euxassay_001572_06	euxassay_001572_07	euxassay_001572_08	euxassay_001572_09

euxassay_001572_10	euxassay_001572_11	euxassay_001572_12	euxassay_001572_13	euxassay_001572_14

euxassay_001572_15	euxassay_001572_16	euxassay_001572_17	euxassay_001572_18	euxassay_001572_19

euxassay_001572_20	euxassay_001572_21	euxassay_001572_22	euxassay_001572_23	euxassay_001572_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17178_wholemount_strong.wlz

17178_wholemount_moderate.wlz

17178_wholemount_weak.wlz

17178_wholemount_possible.wlz

17178_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17178_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 15 16 moderate expression: see section 07 08 09 10 11 12 13 14 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 09 10 22 23 24 weak expression: see section 07 08

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 06

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12

tail dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4772
Entity Detected:	3632451O06Rik, RIKEN cDNA 3632451O06 gene ( MGI:1914669)
Sequence:	sense strand is shown >T4772 GGCGGCCTCTACGTGAGTCGTGGCAGGGATGGCTGGCACTGGCGCTGCCACTTTCCTTCGCGCTCAGCTG TGCTGAATGCTCCAGGACTCAATTACTGGAATTGTCAACTCCTTCAACCTGTTTTTCCCATCCACTATGA GCAGACCAACTCTGATGCCCACCTGTGTGGCTTTCTGTAGCATTCTCTTCCTCACCTTGGCTACAGGATG TCAAGCTTTCCCAAAGGTGGAAAGGAGGGAGACAGCACAAGAATATGCAGAGAAAGAGCAGTCCCAGAAG ATGAACACAGATGACCAAGAAAACATCTCCTTTGCTCCAAAGTACATGCTGCAGCAGATGTCCTCTGAAG CTCCAATGGTACTATCTGAAGGACCTTCAGAGATACCATTACATAAAGTATTCTCAGTCAACAAAGAGAG CCATCTTCCAGGAGCTGGGCTTCTGCATCCTACCAGCCCTGGTGTTTACTCTTCCAGTGAGCCTGCGGTC TCACCCAGTGAACAAGAGCCTGGCCCCAGCCTGCTTGAAAGAATGTCTTCTGAGCACAGCCTTTCCAAGG CCATGTTAACTGTTGCTGTATCTTCACCTGCTTCTCTGAATCCTGACCAGGAGGGACCCTATAACAGTTT GAGTACCCAGCCTATTGTAGCAGCAGTCACAGATGTAACACATGGTTCTCTGGATTATTTAAATAACCAT TTATTTGCAGCTAAAAGTCAGGAAGCAGTTAGCTTAGGAAATTCACCTTCTTCCTCTATAAATACCAAAG AACCTGAGATAATCAAAGCAGATGCAGCCATGGGAACTACTGTCTTTCCTGGTGTTGATTCTACAGGAGA CACAGAGCCTGATAGGGAGAGACCCTCAGAGAATGCAGCTGATGATGGCCAGAGCACTACCACCAAGTAT TTGGTGACAATCCCCAATAATGTCCTGACTACTGAGCCAACAGCCGGCAGTATTTTGGGGGATGCTAAGG TCACAGTGAGTGTCAGCACAGCTGGCCCAGTCTCTTCTATCTTCAATGAGGAGTGGGATGACACCAAATT TGAGAGCATAAGTCGGGGGAGGCCCCCAGAGCCAGGAGACAATGCAGAGACTCAAATGAGAACAAAGCCA CCACATGCGACCTATGAGAGCTTTGAGGGGACTGAGGAGAGCCCCTCTTCCACAGCAGTTTTAAAGGTGG CTCCAGGGCACCTGGGAGGAGAACCAGCCTTGGGAACAGCCCTGGTGACTGCACTGGGGGATGAGAGGTC TCCAGTCTTGACTCATCAGATTTCCTTCACTCCCATGAGTCTGGCAGAAGACCCTGAAGTTTCTACCATG AAGCTGTTTCCAAGTGCAGGAGGCTTCAGAGCCTCCACCCAGGGGGACAGGACCCAGCTCTCCTCAGAGA CTGCATTTTCTACCTCTCAATATGAGTCTGTGCCACAGCAGGAAGCAGGAAATGTGTTAAAAGACATCAC TCAAGAGATAAAAATGGCTACTCAAGCAATGAACACTACATTTCCTGTGGTGACACAAGAGCATATGGCC ACCATCGAGGTTCCCAGAGGCAGTGGGGAGCCTGAAGAAGGCATGCCATCCCTCTCTCCTGTGCCTGCTG AAGTGGCTGATGCTGAGCTGTCCAGAAGATGGGAGTCTCTGGCCACTCCAGCCTCCACCACTGTTGTCCC TTTGTCTCTCAAACTTACCTCTTCCGTGGAAGATCTCATGGACACAATCACTGGACCAAGTGAAGAATTC ATACCAGTTTTGGGCTCTCCAATGGCACCCCCTGCAATGACTGTGGAGGCTCCCACAATTTCTTCAGCAC TCCTTTCCGAGGGAAGAACTAGTCCATCTATCAGCCGCCCAAATACAGCTGCCTCCTATGGCCTAGAACA ACTTGAATCTGAAGAGGTAGAAGATGATGAGGATGAAGAGGATGAAGATGAAGAGGAAGAAGAAGAAGAT GAGGAAGATGAAGAAGATGAGGAAGATAAAGAGACAGACTCCTTGTATAAAGACTTCGATGGTGATACTG AACCACCAGGGTTTACTCTGCCTGGCATCACGTCCCAGGAACCTGACATACGTCCAGGAAGCATGGACCT ACTAGAGGTAGCCACCTACCAGGTGCCAGAGACCATCGAGTGGGAACAGCAGAATCAAGGTCTGGTGAGA AGCTGGATGGAAAAGCTAAAAGACAAGGCAGGCTACATGTCTGGAATGCTGGTGCCTGTTGGGGTAGGGA TAGCTGGAGCCTTGTTCATTTTGGGAGCGCTATACAGCATTAAGGTTATGAATCGCCGAAGAAGAAATGG ATTCAAAAGGCATAAAAGGAAGCAGAGAGAATTCAACAGTATGCAAGACAGAGTGATGCTGTTAGCTGAT AGCTTTGAAGACGAATTTTGAACCGGACTTGAGTTTAACTGAGATATTCCGTGAGATTATTATTGTATTT TCTATTTGAAGGCAAAAAGAAGAAAGAGAATAAATGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5043946 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5043946 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17183 same embryo
	EMAGE:17181 same embryo
	EMAGE:17180 same embryo
	EMAGE:17179 same embryo
	EMAGE:17182 same embryo
	EurExpress:euxassay_001572 same experiment
	MGI:4822705 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source