EMAGE mouse gene expression spatial database

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EMAGE:17189

Ugt1a6b UDP glucuronosyltransferase 1 family, polypeptide A6B ( MGI:3580629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001600. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001600_01	euxassay_001600_02	euxassay_001600_03	euxassay_001600_04

euxassay_001600_05	euxassay_001600_06	euxassay_001600_07	euxassay_001600_08	euxassay_001600_09

euxassay_001600_10	euxassay_001600_11	euxassay_001600_12	euxassay_001600_13	euxassay_001600_14

euxassay_001600_15	euxassay_001600_16	euxassay_001600_17	euxassay_001600_18	euxassay_001600_19

euxassay_001600_20	euxassay_001600_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17189_wholemount_strong.wlz

17189_wholemount_moderate.wlz

17189_wholemount_weak.wlz

17189_wholemount_possible.wlz

17189_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17189_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 03 06 07 08 09 15 16 17

foregut-midgut junction

weak

regional

weak expression: see section 11 12 13

pancreas

moderate

regional

moderate expression: see section 10 11

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 17 18 19 20 21

lateral ventricle choroid plexus

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

4th ventricle lateral recess

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 16

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 16 17 18 19 20

vagus x ganglion

weak

regional

weak expression: see section 08

dorsal root ganglion

weak

regional

weak expression: see section 08 09 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14 15

esophagus

moderate

regional

moderate expression: see section 11 12 13

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08 09

hindgut

weak

regional

weak expression: see section 12 13 14 15

rectum

moderate

regional

moderate expression: see section 12 13

midgut

weak

regional

weak expression: see section 11 12 13 14 15 16

oral epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

kidney calyx

strong

regional

strong expression: see section 09 10 16 17 18

urethra of male

moderate

regional

moderate expression: see section 12 13

trachea

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4785
Entity Detected:	Ugt1a6b, UDP glucuronosyltransferase 1 family, polypeptide A6B ( MGI:3580629)
Sequence:	sense strand is shown >T4785 GGTGGCTGACTAACAACCGACTTCCAGATTTCTTACTCTTGCTCAAGGGCCCTCATCACGTGCTACCTAG AGGCACAGGACTCTAACCTTACTATCTGACAGGATGGCTTGCCTTCTTCCTGCTGCTCAGACACTTCCTG CAGGGTTTCTCTTCCTAGTGCTTTGGGCCTCAGTTCTAGGTGACAAGCTGCTGGTGGTCCCCCAGGATGG AAGCCACTGGCTTAGCATGAAGGAGATAGTGGAGCATCTCAGTGAACGAGGACATGACATTGTGGTCCTA GTGCCAGAAGTCAATTTGCTTTTGGGAGAATCCAAATACTACAGGAGGAAAATTTTCTCAGTTCCCTACA GCCTAGAAGAACTACAGACCCGTTTTCGCACCTTTGGACGCAACCAGTTTGTTCCTGGTGCCCCCCTGAT GGGTCCTCTAAGAGAGTACAGGAACAGCATGCTTACATTGGAGATGTTCTTTTCCAACTGCCAGAGCCTC CTGAAGGACTCTGCCACCCTCAGCTTCCTCAGGGAGAACAAGTTTGATGCTCTGTTCACAGACCCAGCCA TGCCCTGTGGTGTGATCCTGGCTGAGTATCTCAACCTGCCCTCTGTCTACCTCTTCAGAGGTTTCCCATG CTCTCTGGAACACATGCTTGGTCAAAGCCCAAGCCCTGTGTCCTATGTTCCCAGATTCTACACCAAATTC TCAGACCACATGACGTTTCCCCAACGGCTGGCCAACTTCATTGTTAACATCTTGGAAAACTACCTATATT ATTGTCTGTATTCAAAGTATGAAATCATTGCCTCAGACCTCCTCAAGAGAGATGTGTCCCTACCTTCCTT ACACCAGAATTCTCTGTGGCTGTTACGGTACGATTTTGTGTTCGAATATCCCAGGCCGGTCATGCCCAAC ATGATCTTCCTTGGAGGGATCAACTGTAAGAAGAAGGGAAAGCTGACTCAGGAATTTGAAGCCTATGTCA ACGCCTCTGGGGAGCATGGCATCGTGGTTTTCTCTTTGGGATCCATGGTCTCAGAGATTCCGGAGAAGAA AGCCATGGAAATTGCTGAGGCTTTGGGCAGAATTCCTCAGACGGTCCTGTGGCGCTACACCGGAACTAGA CCATCGAATCTTGCAAAGAACACAATTCTTGTCAAATGGCTACCCCAAAATGATCTGCTTGGTCATCCAA AGACTCGGGCATTCATCACACACTCTGGCTCCCATGGTATTTATGAAGGAATATGCAATGGAGTTCCGAT GGTGATGATGCCCCTATTTGGCGATCAGATGGACAATGCCAAGCGCATGGAAACTCGGGGAGCTGGGGTG ACCCTGAATGTCCTTGAAATGACTGCTGATGATTTGGAAAATGCCCTTAAAACTGTCATCAACAACAAGA GCTACAAGGAGAACATCATGCGCCTCTCCAGCCTTCACAAGGACCGTCCTATAGAGCCTCTGGACCTGGC TGTGTTCTGGGTGGAATACGTGATGAGGCACAAGGGGGCACCACACCTGCGCCCGGCCGCCCATGACCTC ACCTGGTATCAGTACCACTCCTTGGATGTGATTGGCTTCCTCCTGGCCATTGTGTTGACAGTGGTCTTCA TTGTCTTTAAATGTTGTGCCTATGGCTGCCGGAAATGCTTTGGGGGAAAGGGGCGAGTGAAGAAATCACA CAAATCCAAGACCCATTGAGAAGTGGGGGGAAGTGAAGGAGAAGTATTAGTTCATTATCTGATCAGTTGA AACTTGGAAACAAGTGTTAGATCCATATTGTTTTTGTTAGGGAAATAATTCACCATACATTATACATTCA GCACATTTAAAAATAATAATAATAACAATCTAATTGCTGGCCACACCCATCAGGGAAGGTTCTAGTATAT GTGATGTGCTTTTCCAGTATCTTCAGTCTAGACAACTCTTGCCATCTGTTGGTAATTTACAGAAAGTCTG GCACTCTGCTTTCAGTGACAGCCCCACAGTTTCCCCTGCTCCCGCCAGCTGATGGCTTTCTCCCCTGGAT TCTCAGACTGCCGTGGCCTTCTCCAGTGTTAGTCATTCTTCATTGTGTTCATGCATTATGGGTAGCAAGA CCTTTGGAGCTTTGGGAGAAGAGATGAGGCTGTGACACTGATGGCCCTGTGTTTGAGATAATAATTGTTG CTTGTGCCGGAATTTGATGAAAACCAAAGTATGTTCTAAGGCAAGTACATCTTCTATTGTGTTCCCAAAC CAAGAACTTATCAATAAATTCATATAAATTGTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5050488 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5050488 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17188 same embryo
	EMAGE:17190 same embryo
	EurExpress:euxassay_001600 same experiment
	MGI:4829085 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source