EMAGE mouse gene expression spatial database

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EMAGE:17201

Ces1e carboxylesterase 1E ( MGI:95432)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001609. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001609_01	euxassay_001609_02	euxassay_001609_03	euxassay_001609_04

euxassay_001609_05	euxassay_001609_06	euxassay_001609_07	euxassay_001609_08	euxassay_001609_09

euxassay_001609_10	euxassay_001609_11

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17201_wholemount_strong.wlz

17201_wholemount_moderate.wlz

17201_wholemount_weak.wlz

17201_wholemount_possible.wlz

17201_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17201_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4797
Entity Detected:	Ces1e, carboxylesterase 1E ( MGI:95432)
Sequence:	sense strand is shown >T4797 CCACGCGTCCGATCATACAATTGATTGAGAGAAATTTGCTGGTACCCTCCAGGAGTGGGGCAGGATCAGT GTGCCCCCTTTGTCACAGGCTGGAGACCTCCCTGTCCTGCAAACCTGTAGCCTCCTACCATGTGCCTCTC TGCTCTGATCCTGGTGTCACTTGCAGCATTCACAGCAGGGGCAGGACATCCATCCTCACCACCCATGGTG GACACCGTGCAAGGCAAAGTCCTGGGGAAGTACATCAGCTTAGAAGGATTCACACAGCCTGTGGCCGTCT TCCTGGGAGTCCCCTTTGCCAAGCCCCCTCTTGGATCTCTGAGGTTTGCTCCACCACAGCCTGCAGAGCC CTGGAGCTCCGTGAAGAATGCCACCTCCTACCCTCCTATGTGCTTCCAAGACCCAGTGACAGGGCAAATA GTCAATGACCTCCTAACTAACAGAAAGGAGAAAATTCCTCTCCAGTTTTCTGAAGACTGTCTCTACCTGA ATATTTACACTCCAGCTGACTTGACAAAGAGTGACAGATTGCCAGTGATGGTGTGGATCCATGGAGGTGG ACTAGTGTTAGGTGGGGCATCAACCTATGATGGACTGGTCCTGTCTACGCATGAAAATGTCGTGGTGGTG GTCATCCAATACCGTTTGGGCATCTGGGGATTCTTCAGCACAGGGGATGAACACAGCAGGGGGAACTGGG GTCACTTGGACCAGGTGGCTGCACTACACTGGGTCCAGGACAACATTGCTAAATTTGGAGGTGACCCAGG CTCTGTGACCATCTTTGGAGAGTCAGCAGGAGGTGAAAGTGTCTCTGTTCTTGTGTTGTCTCCCTTGGCC AAGAATCTCTTCCAGAGGGCTATTTCTGAAAGTGGTGTGGCCCTCACTGCAGGCCTGGTCAAGAAGAACA CCAGGCCTTTGGCTGAGAAAATTGCTGTCATATCTGGTTGTAAAAACACCACTTCCGCTGCCATGGTTCA CTGCCTTCGCCAGAAGACAGAGGAAGAGCTCTTGGGGACCACACTAAAATTGAATCTTTTTAAGCTGGAT TTGCATGGAGACTCCAGACAGAGCCATCCCTTTGTTCCCACTGTGCTTGATGGAGTGTTGCTGCCAAAGA TGCCTGAGGAGATCCTGGCTGAGAAGAACTTCAACACAGTGCCCTACATCGTGGGAATCAACAAGCAAGA GTTTGGCTGGATTCTGCCAACTATGATGAACTACCCACCCTCTGATGTGAAATTGGACCAGATGACGGCC ATGTCTCTCTTGAAGAAGTCCTCTTTTCTTCTTAACCTCCCTGAGGATGCAATTGCAGTGGCCATTGAGA AGTATTTAAGAGATAAAGATTACACAGGCAGAAATAAAGACCAACTTCTGGAATTGATTGGGGATGTGGT ATTCGGTGTACCATCTGTGATTGTGTCCCGTGGACATAGAGATGCCGGAGCCCCCACATACATGTATGAG TTTCAATATAGTCCAAGCTTCTCGTCAGAAATGAAACCAGATACGGTGGTAGGAGACCATGGAGATGAAA TCTACTCTGTCTTTGGGGCCCCAATTTTAAGAGGTGGTACCTCAGAAGAGGAGATCAACCTCAGCAAGAT GATGATGAAATTCTGGGCCAACTTTGCTAGGAATGGGAATCCCAATGGACAAGGGCTGCCCCATTGGCCA GAGTATGACCAAAAGGAAGGTTATCTGCAGATTGGAGCCACCACCCAACAAGCCCAGAAGCTGAAAGAAA AAGAAGTGGCTTTCTGGACTGAACTTCTGGCTAAGAAGCAACTGCCGACAGAACACACTGAGCTGTGAAT GGGAGACTCTGTCAGCCTCACCCTCAGGGGAGCCCAAATGAGACTACTTTACATGGAAGATGATTGTGGC CAAGGCTTTGGGGAGACTGCAGAATTGTGTGGTGGGAGTGGACAGAGGCCAGAGAGAGAATATTTGCACA TGTGGACTCAATTTGAAAAAATAAATTTTGTTCCATAAGTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5052893 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5052893 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17203 same embryo
	EMAGE:17202 same embryo
	EMAGE:17200 same embryo
	EMAGE:17199 same embryo
	EurExpress:euxassay_001609 same experiment
	MGI:4824621 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source