EMAGE mouse gene expression spatial database

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EMAGE:17208

Pcx pyruvate carboxylase ( MGI:97520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001613. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001613_01	euxassay_001613_02	euxassay_001613_03	euxassay_001613_04

euxassay_001613_05	euxassay_001613_06	euxassay_001613_07	euxassay_001613_08	euxassay_001613_09

euxassay_001613_10	euxassay_001613_11	euxassay_001613_12	euxassay_001613_13	euxassay_001613_14

euxassay_001613_15	euxassay_001613_16	euxassay_001613_17	euxassay_001613_18	euxassay_001613_19

euxassay_001613_20	euxassay_001613_21	euxassay_001613_22	euxassay_001613_23	euxassay_001613_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17208_wholemount_strong.wlz

17208_wholemount_moderate.wlz

17208_wholemount_weak.wlz

17208_wholemount_possible.wlz

17208_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17208_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

homogeneous

moderate expression: see section 07 08 09 15 16 17

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 18 19

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 08 09 12 13 14

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 12 13 14 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

cervical ganglion

moderate

regional

moderate expression: see section 08 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12 13

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4804
Entity Detected:	Pcx, pyruvate carboxylase ( MGI:97520)
Sequence:	sense strand is shown >T4804 GGGCCGTCAGAGGCGGCGGCCACGGCTAGAGGCGGCAGGGCGGAGCTAACATCTACCTTCTGGAGAGCTG ACAACACTAATGATGCTGAAGTTCCAAACAGTTCGAGGGGGCCTGAGGCTCCTGGGTGTCCGCCGATCCT CCTCGGCCCCTGTTGCCTCCCCAAATGTCCGGCGTCTGGAGTATAAGCCTATCAAGAAAGTAATGGTGGC CAACAGAGGTGAGATTGCCATCCGAGTGTTTCGTGCGTGCACAGAGCTGGGTATCCGCACAGTGGCTGTC TACTCGGAGCAGGACACAGGGCAGATGCACAGGCAGAAAGCTGATGAAGCCTACCTTATTGGCCGTGGCC TGGCACCTGTGCAGGCCTACCTGCACATTCCAGACATCATCAAGGTGGCCAAGGAAAATGGTGTAGATGC GGTGCATCCTGGCTATGGGTTCCTCTCAGAGCGAGCAGACTTTGCCCAGGCCTGCCAAGATGCTGGAGTC CGGTTCATTGGTCCAAGCCCAGAGGTGGTCCGCAAGATGGGAGACAAGGTGGAAGCCCGGGCCATTGCCA TCGCTGCAGGCGTTCCAGTGGTCCCCGGCACGGACTCCCCCATCAGCTCCCTGCACGAGGCGCATGAGTT CTCCAACACCTTCGGCTTCCCTATTATCTTCAAGGCCGCCTACGGAGGTGGGGGCCGCGGCATGCGGGTC GTGCATAGCTATGAGGAGTTGGAAGAGAATTACACCCGGGCCTACTCCGAGGCCTTGGCAGCCTTTGGGA ATGGAGCGTTGTTTGTGGAGAAGTTCATTGAGAAGCCAAGGCACATTGAGGTGCAGATCCTAGGGGACCA GTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGTCGGCACCAGAAGGTGGTAGAG ATCGCCCCTGCTACCCACCTGGATCCCCAACTTCGCTCACGTCTCACCAGTGACTCTGTCAAACTTGCCA AGCAGGTAGGCTATGAGAACGCCGGCACTGTGGAGTTCCTGGTGGACAAGCACGGCAAGCACTACTTCAT CGAGGTCAATTCCCGCCTGCAGGTGGAGCACACGGTCACCGAGGAGATCACAGATGTGGACCTGGTCCAT GCTCAGATCCACGTGTCCGAAGGCCGGAGCCTGCCTGACCTGGGCCTGCGGCAGGAGAACATCCGCATCA ATGGCTGTGCCATTCAGTGTCGGGTCACCACCGAGGACCCTGCACGCAGCTTCCAGCCAGACACCGGCCG CATTGAGGTTTTCCGGAGTGGTGAGGGCATGGGCATCCGCCTGGACAACGCCTCTGCATTCCAGGGCGCT GTCATATCGCCCCACTATGACTCTCTGCTCGTCAAGGTCATTGCACACGGCAAAGACCACCCCACAGCTG CCACCAAGATGAGCAGAGCCCTGGCCGAGTTCCGTGTCCGAGGTGTAAAGACCAACATCCCCTTCCTGCA GAATGTTCTCAACAACCAGCAGTTCCTGGCAGGCACAGTGGACACCCAGTTCATCGATGAGAACCCTGAG CTGTTCCAGCTTCGGCCTGCACAGAACCGGGCCCAGAAGTTGCTACATTACCTCGGACATGTCATGGTGA ATGGCCCTACCACTCCAATCCCTGTCAATGTGAGCCCCAGTCCTGTGGATCCTGCTGTTCCTGTGGTGCC CATAGGCCCACCTCCAGCTGGTTTCAGGGACATCCTTCTGCGAGAAGGGCCAGAGGGCTTTGCCCGAGCT GTGCGGAATCACCAGGGGCTGCTGTTGATGGACACAACCTTCCGGGATGCCCACCAGTCACTACTGGCCA CTAGAGTGCGCACACATGATCTCAAAAAGATTGCGCCCTATGTTGCCCACAACTTCAACAAGCTCTTCAG CATGGAGAACTGGGGAGGCGCCACGTTCGACGTTGCCATGCGCTTCCTGTACGAGTGCCCCTGGCGGCGG CTCCAGGAGCTCCGGGAGCTTATCCCGAACATCCCGTTCCAGATGCTACTGAGGGGGGCCAATGCTGTGG GCTACACCAACTACCCTGACAACGTGGTCTTCAAGTTCTGTGAGGTGGCCAAAGAGAATGGTATGGACGT CTTCCGAGTCTTTGACTCCCTCAACTACTTGCCAAACATGCTGCTGGGCATGGAAGCAGCAGGCAGTGCT GGGGGTGTGGTGGAGGCTGCCATCTCATACACGGGGGACGTGGCTGACCCTAGTCGCACTAAATACTCAC TGGAGTACTACATGGGCTTAGCTGAAGAACTGGTGCGAGCTGGCACTCACATCCTGTGCATTAAGGACAT GGCGGGCCTGCTGAAGCCTGCCGCCTGCACCATGCTGGTCAGCTCCCTCCGGGACCGATTCCCCGACCTC CCACTGCACATCCATACCCATGATACATCAGGGGCAGGTGTGGCAGCCATGCTGGCCTGTGCACAAGCAG GGGCTGATGTTGTGGACGTGGCAGTAGACTCCATGTCTGGGATGACCTCACAGCCAAGCATGGGGGCCCT GGTGGCCTGTACCAAAGGGACTCCTTTGGACACAGAGGTACCCCTGGAGCGTGTGTTTGACTACAGTGAG TACTGGGAAGGGGCTCGGGGACTGTACGCAGCCTTCGATTGCACGGCTACCATGAAGTCTGGCAACTCCG ACGTGTATGAGAATGAGATTCCAGGGGGCCAGTACACCAACCTGCACTTCCAGGCCCATAGCATGGGGCT TGGCTCCAAGTTCAAGGAGGTCAAGAAGGCCTATGTGGAGGCTAACCAGATGCTGGGGGACCTCATCAAG GTGACACCATCCTCCAAGATTGTGGGGGACCTGGCCCAGTTCATGGTGCAGAATGGGTTGAGCCGGGCAG AGGCAGAAGCTCAGGCAGAAGAGCTGTCCTTCCCCCGCTCTGTGGTGGAGTTCCTGCAGGGCTACATTGG CATTCCCCATGGGGGTTTCCCTGAGCCCTTTCGCTCTAAGGTGCTAAAGGACCTGCCAAGAATAGAGGGG CGGCCTGGAGCCTCCCTCCCTCCCCTGAACCTGAAGGAGCTGGAGAAGGACCTGATTGATAGGCATGGGG AGGAGGTGACCCCAGAGGACGTCCTCTCTGCAGCCATGTACCCTGATGTCTTTGCTCAATTCAAAGACTT CACGGCTACCTTCGGCCCCCTGGATAGCCTTAATACTCGTCTCTTTCTTCAAGGACCCAAAATTGCAGAG GAGTTTGAGGTTGAGCTGGAACGGGGCAAGACCCTGCACATCAAAGCCCTGGCTGTAAGCGACCTGAACC GTGCTGGCCAGAGGCAGGTGTTCTTTGAACTCAATGGGCAGCTTCGATCCATTCTGGTTAAAGACACCCA GGCCATGAAGGAGATGCACTTCCATCCCAAGGCTTTGAAGGATGTGAAGGGCCAAATTGGGGCCCCGATG CCTGGGAAGGTCATAGACATCAAGGTGGCAGCAGGGGACAAGGTGGCTAAGGGCCAGCCCCTCTGTGTGC TCAGCGCCATGAAGATGGAGACTGTGGTGACTTCGCCCATGGAGGGCACTATCCGAAAGGTTCATGTTAC CAAGGACATGACTCTGGAAGGCGACGACCTCATCCTAGAGATTGAGTGATCTTACTCCAGCCTGGCAGCC TGGCCGACCCTACCCCAAGCCTCTCAACAGAAGCTGTGCAGCCAGGGCAGGCCCAGGCCAGCCAGTACCT GAGAGTAGGAAGGCCAGGCCCTTGAGGTCCTGTCCCATGGGACAGCACACACACTACCTGCAATGGCCTG CCCATTCCCTTCAGCTATTTGTCCTTTTCTTACTGGCAGGTAGTTGCTCACATATTCATCTCTTGCCAAA TAAGGGCCTGCTCCTCGTGGGAGACCACAGGTGTACAGTCGGTGGCCTTGTACCTGGGGGAGGTGGCTTA GGGCTTCTACCTCTGGGGGTAGAGGGAAGACCTAATTCACAGGTCCTGGGAAATTTGCTCAATAAAAGTG GCCTTCCCTTGCCCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5053475 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5053475 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17209 same embryo
	EMAGE:17207 same embryo
	EurExpress:euxassay_001613 same experiment
	MGI:4827105 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source