EMAGE mouse gene expression spatial database

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EMAGE:17221

BC025446 cDNA sequence BC025446 ( MGI:2385015)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008875. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008875_01	euxassay_008875_02	euxassay_008875_03	euxassay_008875_04

euxassay_008875_05	euxassay_008875_06	euxassay_008875_07	euxassay_008875_08	euxassay_008875_09

euxassay_008875_10	euxassay_008875_11	euxassay_008875_12	euxassay_008875_13	euxassay_008875_14

euxassay_008875_15	euxassay_008875_16	euxassay_008875_17	euxassay_008875_18	euxassay_008875_19

euxassay_008875_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17221_wholemount_strong.wlz

17221_wholemount_moderate.wlz

17221_wholemount_weak.wlz

17221_wholemount_possible.wlz

17221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

strong

regional

strong expression: see section 16 moderate expression: see section 07 08 09 10 11 12 13 14 15 17 18

kidney calyx

strong

regional

strong expression: see section 08 17 18 19 moderate expression: see section 09 10 16

kidney pelvis

moderate

regional

moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4807
Entity Detected:	BC025446, cDNA sequence BC025446 ( MGI:2385015)
Sequence:	sense strand is shown >T4807 CCACGCGTCCGTGAGCTCTGTGAGAGCCCAGGGATGTGATTGGTGATTTATTAACTGGCTCCAACTTCCA AGGTTTTATCTGTGTACCCTTTCTCCAAGGATGGACAGTTCTCATGCTACAAGGACCTGTGTGTTCATCC TTCTTGTGGCCCTATTGTGTGCAGAAGGAGCTCAGGGGCTGGAGTGCTACTGTTGCCTGGGGGTCCCACT TGAGACTTCCTGCAAAACAATGACCTGCCCCTACTCTGATGGATTCTGTGTTACTCAGGAAGAAGAAGTT ATTGTGGACTCTCACACAATCAAAGTAAAGAGCAATCTCTGCCTTCCCATCTGCCCTACTAATCCTGAAA CTACTGAGTTCCTGGGTAATGCTGTCAACGTGAAGACTTTCTGTTGCAAGGAAGACCTCTGCAATGCAGC AGTTCTCACTGAAGGCAGCACCTGGACCATGGCAGGGGAGTCCTCATGGAAATAGACAACACCCTGGAAA CTTGGGAGCTATGACTATTACTCCCTGGTCTTGAAGGTGCTAAAGATCTGAGATTTTATCATGATGAAGA TCCTTTAAGAAAGAACTATCACTCAAAGAGGGAAGTCCTCATCTCTGCAGCCTTCTCCTCTGGGACAAGC ATAGGATCCAGGAATGTGGCCAAGGTGCTGGTTGGGACATGAAACTTGAAACATGAGTAGGACTACTGGA TATTTCTTCCATATTTGGCCTTAATTCTTTTGCAGTCTCTTCTGGCCTCTGCTTTCAAGTGCTGGGTTTT GCAGGTATGTACTCATTACCACACTGGCTATTCCTTGTGCTTTAAAAGGGCAGACTGCACCTGACCTCAC TGGCTGAGGCACTCAGGCCATGCAGGAGAGCTGGCCCCAATGGCATGAGCACAGGAAAGCTGATCCTGCC TCTTGCCAGCTGCCATAGGCAGGACAGCTGTCCCTGACCCCTGCCTGTGCTAAGCAGGAGAGCTGCTCTT GGAAGTTTGGGTGCAGGAGAGCTGGTGGGATGACCAACTCAATTACCACCCATGGCCAGATCCAAGCCTT AGGGTTGGCCCAGCCCAACATCTACTCCATCTATGAACTGCTGGTAAACATGAAGATGCTGGTCCTGCAG ATCCAAAAATACAGGATCTCCATGACACAGGGTAACAACAACAGAGAGGGATTCTGGGACAAACCCAGTA TTGATAGTGTAGCAGAAGCCAGAAGCCTCTAACCAGAGCAATGACTCATTTAATGATAATCTGTGAGTAA AATTGTTTGGAAAAAAGGAGTATACTGTGGAGCCCACTGTGGCACATCAGACCTACCATGTTGAGATGTT TTTCTTTTTTTTTCTTTTTTTTTTTTTTATATTTTGAAGGGGAGGTTGCAAGGGTATAGAATGATTATGG AGGGATGGCGATATGAGTGGAACTGAAGTGCATGTTGGGAAATTCACAAAGAATCAATAAAATATTTTTA TATATGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5053382 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5053382 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17223 same embryo
	EMAGE:17226 same embryo
	EMAGE:17224 same embryo
	EMAGE:17225 same embryo
	EMAGE:17222 same embryo
	EurExpress:euxassay_008875 same experiment
	MGI:4823418 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source