EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17234

Acss2 acyl-CoA synthetase short-chain family member 2 ( MGI:1890410)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001617. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001617_01	euxassay_001617_02	euxassay_001617_03	euxassay_001617_04

euxassay_001617_05	euxassay_001617_06	euxassay_001617_07	euxassay_001617_08	euxassay_001617_09

euxassay_001617_10	euxassay_001617_11	euxassay_001617_12	euxassay_001617_13	euxassay_001617_14

euxassay_001617_15	euxassay_001617_16	euxassay_001617_17	euxassay_001617_18	euxassay_001617_19

euxassay_001617_20	euxassay_001617_21	euxassay_001617_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17234_wholemount_strong.wlz

17234_wholemount_moderate.wlz

17234_wholemount_weak.wlz

17234_wholemount_possible.wlz

17234_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 15 16 17

spinal cord

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 05 06 12

cervical ganglion

moderate

regional

moderate expression: see section 05 06 14

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 13 14 15 16 17

neural retina

moderate

regional

moderate expression: see section 01 02 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5649
Entity Detected:	Acss2, acyl-CoA synthetase short-chain family member 2 ( MGI:1890410)
Sequence:	sense strand is shown >T5649 GTTTTCCCCGCCCCGGCACCCGCAGCGACCGCAACGGCGGACAAGGTGTTCGGAACTTGATGCGATGGGG CTTCCCGAGGAGCGGCGCAAGAGCGGCAGTGGAAGCCGGGCTCGTGAGGAGACCGGCGCCGAGGGCCGGG TGCGGGGTTGGTCCCCGCCGCCGGAGGTCAGACGCTCGGCGCACGTCCCCTCTCTGCAGCGCTACCGCGA GCTGCACCGGCGTTCTGTGGAGGAGCCACGGGAGTTTTGGGGAAACATTGCCAAGGAATTTTACTGGAAA ACTGCATGCCCTGGCCCATTCCTCCAGTACAACTTTGATGTGACTAAAGGGAAAATATTCACTGAGTGGA TGAAAGGAGCAACTACAAACATCTGCTACAACGTGCTGGATCGAAATGTCCATGAGAAAAAACTTGGCGA CAAAGTTGCTTTTTACTGGGAGGGCAATGAGCCAGGGGAGACCACCAAGATCACATACCGTGAACTCCTG GTCCAGGTGTGTCAGTTCAGCAATGTTCTCCGTAAACAGGGCATTCAGAAGGGTGACCGAGTGGCCATCT ACATGCCTATGATCTTGGAACTTGTGGTGGCTATGCTGGCATGTGCTCGCCTTGGAGCTTTGCACTCCAT TGTGTTTGCAGGCTTCTCTGCAGAGTCTCTCTGTGAAAGGATCTTGGATTCCAGTTGCTGCCTGCTCATC ACTACAGATGCCTTCTACAGGGGGGAAAAACTTGTGAACCTGAAGGAGCTGGCTGATGAGTCCTTGGAGA AGTGCCGAGAGAAGGGCTTCCCAGTGAGATGCTGCATTGTGGTCAAACATCTGGGGCGGGCAGAGCTGGG CATGAATGACTCCCCCAGCCAGTCCCCACCAGTTAAGAGGCCATGTCCAGATGTCCAGATCTGCTGGAAC GAAGGGGTTGACTTATGGTGGCATGAACTCATGCAGCAGGCAGGAGACGAGTGTGAGCCTGAGTGGTGTG ATGCTGAGGACCCACTCTTCATCTTGTACACCAGTGGCTCCACAGGCAAACCTAAGGGTGTGGTGCACAC AATTGGAGGCTATATGCTCTATGTGGCTACAACTTTCAAGTATGTGTTTGATTTCCACCCGGAAGATGTG TTCTGGTGCACAGCAGACATCGGCTGGATCACTGGTCATTCCTATGTCACCTATGGGCCACTGGCTAATG GTGCCACTAGTGTTTTGTTTGAGGGGATCCCCACATACCCAGATGAAGGGCGCTTGTGGAGCATTGTGGA CAAATACAAGGTGACCAAGTTCTACACGGCACCAACAGCCATCCGGATGCTCATGAAGTTTGGAGATGAT CCTGTCACCAAGCATAGCCGGGCATCCCTGCAGGTGCTGGGCACAGTAGGTGAACCCATCAACCCTGAAG CCTGGCTATGGTACCACCGGGTAGTAGGTTCCCAGCGCTGCCCCATTGTAGACACCTTCTGGCAAACAGA AACAGGTGGCCATATGCTGACCCCTCTCCCTGGCGCCACACCCATGAAACCTGGTTCTGCTTCTTTCCCA TTCTTCGGTGTAGCGCCTGCAATCCTGAATGAGTCCGGGGAGGAGCTGGAAGGGGAAGCTGAAGGTTATC TGGTGTTCAAGCAGCCCTGGCCAGGGATCATGCGCACAGTCTATGGGAACCACACACGGTTTGAGACCAC CTACTTTAAGAAGTTCCCTGGCTACTATGTGACCGGAGATGGCTGCCGACGGGATCAGGATGGCTATTAC TGGATCACGGGCAGGATTGATGACATGCTCAATGTGTCTGGACATCTCCTGAGTACAGCAGAGGTGGAAT CGGCACTTGTGGAACACGAGGCTGTCGCAGAGGCAGCTGTGGTGGGCCACCCTCATCCTGTGAAGGGCGA ATGCCTCTACTGCTTTGTTACCTTGTGTGATGGCCATACCTTCAGCCCCACACTCACAGAGGAACTCAAG AAGCAGATTAGAGAAAAGATTGGCCCCATTGCCACACCAGACTACATCCAGAATGCACCTGGCTTGCCTA AAACACGCTCAGGGAAAATCATGAGGCGAGTTCTCCGGAAGATTGCTCAGAATGACCATGACCTGGGGGA TACATCTACGGTGGCTGACCCATCTGTCATCAACCATCTCTTCAGTCACCGCTGCCTGACCACCCAGTGA ACAAGACTCTTGTCCAGGATTTCCCTGTGCTTGGCTCTCCAAATTTCTGCCCATCCATCCTGCCCCTCAC ACAGTAGTACTAGTTGACAGTACTAGTTGGTTGAAACTAGGGAGACAGTTACCTAGACTACCAGGTAGAA GGGACAGGGGTCAGGTCAACCTCACACTGATATGCTCCTCGACTAGAGAGGTCTCCAAGCCCAGAACAGA GACTTGAACACCATGTCCTTAACCAAAGTCAGCTCTTCAGAGTGTAAATGAGGGTCTGACTGGAAGCAGG GCAGCAGCTGTAATTCTTCCAGCTCTCTCCAAAAGCACCAGTTCTTATCTTGGGCCTGCACTAGGCTTTA GAAATGACAGTTTGTGAGTCTATGAATAATTATTTTTTTAAAAACTTTGTTGTCTCTGTTCTGAGTCTGA ATTCCTTGCAGATGGTAGCATTGTCTACCAGTTGGTTCAGTCTCCATAGGACTTTCATATCATCTGCTAC ATTGAAAGAATAAGATATACTGATCCTAAAACCAAGGGAATTAACATTCCTAATCCTGCACTATGAACAG AACCCTGCCCATGAGAGGCACTTGTAGGTGAAACTCTCTTTTCTTGTTCTTTTTGTGCTCAAGACCCACT GTTATAGGAATAAAGTTTATGAGCTCAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6515568 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6515568 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17237 same embryo
	EMAGE:17236 same embryo
	EMAGE:17232 same embryo
	EMAGE:17235 same embryo
	EMAGE:17233 same embryo
	EurExpress:euxassay_001617 same experiment
	MGI:4822954 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source