EMAGE mouse gene expression spatial database

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EMAGE:17248

Tmed2 transmembrane emp24 domain trafficking protein 2 ( MGI:1929269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003295_01	euxassay_003295_02	euxassay_003295_03	euxassay_003295_04	euxassay_003295_05

euxassay_003295_06	euxassay_003295_07	euxassay_003295_08	euxassay_003295_09	euxassay_003295_10

euxassay_003295_11	euxassay_003295_12	euxassay_003295_13	euxassay_003295_14	euxassay_003295_15

euxassay_003295_16	euxassay_003295_17	euxassay_003295_18	euxassay_003295_19	euxassay_003295_20

euxassay_003295_21	euxassay_003295_22	euxassay_003295_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17248_wholemount_strong.wlz

17248_wholemount_moderate.wlz

17248_wholemount_weak.wlz

17248_wholemount_possible.wlz

17248_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17248_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5654
Entity Detected:	Tmed2, transmembrane emp24 domain trafficking protein 2 ( MGI:1929269)
Sequence:	sense strand is shown >T5654 GCTGAGGCGGCAGCGGTGGCGGCGGCTCCGGAGGCCGCAGTCCGCGTCCCGGCGTCGGCCCGTCCCGCAC CATGGTGACGCTCGCCGAGCTGCTGGCGCTGCTGGCCGCGCTGCTGGCCACGGCCTCGGGCTACTTTGTC AGCATCGACGCGCACGCCGAGGAGTGCTTCTTCGAGCGGGTCACCTCCGGCACCAAGATGGGCCTCATCT TCGAGGTGGCGGAGGGCGGCTTCCTGGACATCGACGTGGAGATCACAGGACCAGATAATAAAGGAATCTA TAAAGGAGACCGGGAGTCCAGCGGGAAGTACACATTTGCAGCCCACATGGATGGGACATACAAGTTCTGC TTTAGCAATAGGATGTCCACTATGACTCCAAAGATAGTAATGTTCACCATTGACATTGGGGAGGCTCCCA AAGGACAAGACATGGAGACAGAAGCTCATCAGAACAAGCTAGAAGAAATGATTAATGAGCTGGCAGTGGC AATGACAGCCGTAAAGCACGAACAGGAGTACATGGAAGTCCGGGAGAGAATACACAGAGCCATCAATGAC AACACAAACAGCAGAGTGGTCCTTTGGTCCTTCTTCGAAGCTCTTGTTCTAGTTGCCATGACATTGGGAC AGATCTACTACCTGAAGAGATTTTTTGAAGTCCGGAGGGTTGTTTAAAAGGCCTTTTCCTGTTGATCCCA AATTCATGATTTACTTGTTTACCAAACACCTTGGTCATAATAATGTCATTAGTTTATACATTTTTATTTT CTGAACGTTCATGACTTATGTTTCTTTGTGATTAGTAGGTGTTGGGGAAAACCTGTTTAAGAGAGTTAGA GTGAAAATTTGACATTTGATTGGCCTATAATTCTTACTTGAAAGGAGCCCCATTATATAGGCCCTTCCAA GAATCCAGAGGCTGTTAATGCTGTATGGGACTATGGTCTTTATTATGTTTTTACCTTTGGTTTTCATAAT AAAATGCAGTTCATCCAGGAAGTAGCCCACAGCCTGACAATACTTGTTAACTAACTGCATTCTAGTAGTC CCTTGGTTCATCATCAGAGGCTCATTTTGTTATTACAGCAACAGCAATCTGAATCTTGACCCTGTGGCCC CGCCTCAGGGTGGAGAGTCCTGATGGGGCCTGTTACCAGGGTGGGCACTCATGCTTTCTCTTGAGCTCGA TTTAAAGACTCATCAAAACTCTTCCCCCTAAAGTTTTATATTGAACCTTAGAACTGAATCACCTCTAATT GAATTGAACAAGCAGCCAATAACTTAGCCGTTCTTTGAATCCAGTTTCCCAGGTGTTGGAACAGACTGCT GTCCTGTGTGCTGCAGAGTCTGTCCCTTGTTGGCACTTAGGAAGATCCAGTTTCTCCCTCACTGGAAGTC TGCACTTCTCAAGCAGAAGGATTAAGCCTCTGGGGTTGCTTGCAGTGATAGGCAGCCACTGATGTCTTTT TAGTCAGTGGCCTTTGGGCCCTGGTCTTCAGTGTCAGCACTAGGGTTTGTTTGTCCTCTGGGGTCTTTTT TGGCACATGTCAGTTTTGTTTATATAAAATGAAGAGACTGTCTCTCTGATGATGGGTTTAATGAAAGGCA CTCTGCTTTGGGTGTGGGCGGTCCAGGATTATGTGGTAACTATTGTCTGTTAGTTACCTGGATATTTTAT TTTTCTTTTTGGATCTTGCAAGGGGAAAACTACAAGTAACAAGTTTTATAAATTTAATTTAAATTGTTAC AGGTTTTCATGTTCAGGACAAATAATTTTTCAACCTTCGATGATATCCTTGCAGCAGTTTGTTGGTAAGG TGACTTTCACCTAGGGCAGCTGTTGCATGCCAATTTTTTTTTTTTGTGTGTGTGTGTGAAAACACTTCAG AATTGAATTAAAAGATGTAAATTTAAAATTGGTTGTGTATCTAATATGCCCAGGTTCAGTAAATAAACAA TTCTTTTTAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6528722 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6528722 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17249 same embryo
	EMAGE:17250 same embryo
	EurExpress:euxassay_003295 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source