EMAGE mouse gene expression spatial database

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EMAGE:17254

Slc9a1 solute carrier family 9 (sodium/hydrogen exchanger), member 1 ( MGI:102462)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001642. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001642_01	euxassay_001642_02	euxassay_001642_03	euxassay_001642_04

euxassay_001642_05	euxassay_001642_06	euxassay_001642_07	euxassay_001642_08	euxassay_001642_09

euxassay_001642_10	euxassay_001642_11	euxassay_001642_12	euxassay_001642_13	euxassay_001642_14

euxassay_001642_15	euxassay_001642_16	euxassay_001642_17	euxassay_001642_18	euxassay_001642_19

euxassay_001642_20	euxassay_001642_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17254_wholemount_strong.wlz

17254_wholemount_moderate.wlz

17254_wholemount_weak.wlz

17254_wholemount_possible.wlz

17254_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17254_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 13

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11

hindgut

moderate

regional

moderate expression: see section 13

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5662
Entity Detected:	Slc9a1, solute carrier family 9 (sodium/hydrogen exchanger), member 1 ( MGI:102462)
Sequence:	sense strand is shown >T5662 TGAACTTTTCCTAGAGATTGCCCCAGTCTCACCCCATCGGCAGGACTCCTTGCTACCTATGTCCCAGGTC TTACACGCACTTCGCTAAGGGCTGAGGCCTCCTAGGCTGGGGGATCAGTATGATGCTTCGGTGGTCCGGC GTCTGGGGATTTCATCCACCTCGGATCTTCCCCTCCTTGCTGATGGTGGTTGCCTTGGTGGGGCTGCTAC CTGTTCTCAGGAGCCACGGCCTCCAGCACAGCCCTACTGCCAGCACCATCAGAGGTTCTGAACCACCCCG GGAACGCTCAATTGGGGATGTCACCACGGCACCATCAGAGCCTCTCCATCGCCCAGATGACCACAATTTG ACCAACTTAATCATTGAACATGGCGGTAAGCCATCTCGGAAGGCCTTCCCAGTCCTGGACATTGACTACC CTCACGTGCGCACACCCTTTGAGATCTCCCTCTGGATTCTCCTGGCCTGCCTCATGAAGATAGGTTTCCA TGTGATCCCCACCATCTCAAGCATCGTCCCGGAGAGCTGCCTGCTGATCGTAGTGGGGCTGCTGGTGGGG GGCCTGATCAAGGGCGTCGGAGAGACGCCCCCCTTCCTGCAATCAGACGTCTTCTTCCTCTTCCTGCTGC CACCCATCATCCTGGATGCAGGCTACTTCCTGCCTCTGCGGCAGTTCACGGAGAACCTGGGCACCATCCT GATCTTTGCTGTGGTGGGCACACTGTGGAATGCGTTCTTCCTGGGTGGCCTCCTGTACGCCGTGTGCCTG GTGGGCGGCGAGCAGATCAACAACATTGGCCTGCTGGACACCCTCCTCTTTGGCAGCATCATCTCCGCCG TGGACCCCGTGGCTGTGCTGGCCGTCTTCGAGGAGATCCACATCAACGAACTGCTACACATCCTTGTCTT CGGGGAGTCCCTGCTCAACGATGCCGTCACTGTGGTCCTCTATCACCTCTTTGAGGAATTTGCCAGCTAT GACTCTGTGGGCATCTCGGACATCTTCCTCGGCTTCCTGAGCTTCTTCGTGGTGGCCCTCGGTGGGGTGT TTGTGGGCGTGGTCTACGGGGTAATCGCAGCTTTCACCTCCCGATTTACCTCCCACATCCGGGTCATCGA GCCGCTCTTCGTCTTCCTCTACAGCTACATGGCTTACCTGTCAGCTGAGCTCTTCCACCTGTCCGGAATC ATGGCCCTCATCGCCTCAGGAGTAGTGATGCGCCCCTATGTGGAGGCCAACATCTCCCACAAATCCCACA CCACCATCAAGTACTTCCTGAAGATGTGGAGCAGCGTCAGTGAGACCCTCATCTTCATCTTCCTCGGCGT CTCCACTGTGGCCGGCTCCCACCAGTGGAACTGGACCTTCGTCATCAGCACCCTGCTCTTCTGCCTCATC GCCCGGGTGCTGGGTGTCCTGGTCCTGACCTGGTTCATCAACAAGTTCCGCATTGTCAAGCTGACCCCCA AGGACCAGTTCATCATTGCCTATGGGGGCCTGCGAGGGGCCATCGCCTTCTCGCTGGGCTACCTCCTGGA CAAGAAGCACTTCCCCATGTGTGACCTGTTCCTCACCGCCATCATCACCGTCATCTTTTTCACCGTCTTT GTGCAGGGCATGACCATTCGGCCCCTGGTCGACCTGTTGGCCGTGAAGAAAAAGCAAGAAACAAAGCGCT CCATCAACGAGGAGATCCACACGCAGTTCCTGGACCACCTTCTGACAGGCATCGAGGACATCTGTGGTCA TTATGGCCACCATCACTGGAAGGACAAGCTCAACCGTTTTAATAAGAAGTATGTGAAGAAGTGCCTGATA GCAGGAGAGCGCTCCAAAGAGCCCCAGCTCATTGCCTTCTACCACAAGATGGAGATGAAGCAGGCCATTG AGCTGGTGGAGAGCGGAGGCATGGGCAAGATCCCATCTGCTGTCTCCACTGTCTCTATGCAGAACATCCA CCCCAAGGCTGTGACTTCAGACCGCATCTTGCCAGCACTGTCCAAGGACAAGGAGGAAGAGATCCGCAAA ATCTTGAGGAGCAATCTGCAGAAAACCCGGCAACGGCTGCGGTCCTATAACAGACACACGCTGGTGGCCG ACCCCTACGAGGAAGCCTGGAACCAGATGCTGCTCCGGAGGCAGAAGGCCCGGCAGCTGGAGCAGAAGAT CACCAACTACCTGACAGTACCGGCCCACAAGCTGGACTCGCCCACCCTGTCTCGGGCCCGCATAGGCTCA GACCCACTGGCCTATGAACCCAAGGCAGACCTGCCTGTCATCACCATTGACCCGGCCTCCCCACAGTCCC CTGAGTCTGTGGACCTGGTGAACGAGGAGTTGAAGGGCAAGGTCCTAGGGCTAAACCGGGGTCCCAGGGT GACTCCGGAGGAGGAGGAGGAAGATGAGGACGGCATCATCATGATTCGGAGCAAGGAGCCCTCATCCCCA GGCACCGACGACGTCTTCACCCCTGGATCAAGTGACAGCCCCAGCTCCCAGAGAATACAACGCTGCCTCA GTGACCCAGGCCCCCACCCTGAGCCTGGGGAGGGAGAGCCTTTCATCCCCAAAGGACAGTAGAGCCAGGC TAGTGGGTGTCCCACAGACTCTCCCACGAGAGTGGGGGCTGGGCACTCCTCTTGTCTCCTGACCTGGATT GGCTCTGCCCCCTTCCTCCGCATGGCAGCTGGGCCCACAGCCCCCACTGCAGCACGGCTTGCCCCTGCCT CCTGGGAAGGGTCCTCTCCTACAGAACTGCCTTCCCAATCCATCCATTTGGCGGACGGCTGGGCTGCTGA GGCAGGCCCCGCCCCATCTCTAGATCCAGGCTCTCCCATAGTGGAACCAGGGCTCCTCAAGGCCCTCCTT CCCTCCAGCCCCTCCTCATCCAGACCAACTCTCCATCCTTGATCATCATTTCTAACCAAAGAGCCACTGG CTCCACTGTGGTCCCAGCAGGAAAGGAGGGAGCTGAGCCTTCCGTGGCCGTGACTGGGCCCCTGTATCAG AGGACAGCAGGCCTGTGGCTGGGAAACAGACCCGTGACCCATAGCAGAGCTGCCCACGGCAGCGCCACGG TTCTCCCGCTAGCCTCGGTGCTCTGGCCACCACCCGAGCTGTCCTTACTCAGAGCTGCGGGCTGAGGGCG TCTCTGAGCTTCTCTGCTGGGCGCTGGGGCACATTCGACAGTGACATGATTGGGTGATGAGGACCACTGG GCCTTAGTGGGGCTTACACGGGAGGGACTGTCCGAGGCGCTGGAAAGTGTCCTCTACTTACACTTGGCTT ATTTGCACCTCACTGTAAGTATGATTCTGTTTGCACAGAGGCACTCTTGTTTTCAGACCCTTTGAGTCCC CCATGCCAGTCAGAGCTCTGCCCTTTCCCCCTCGTCCTTCCTCGTGTCTAACACCGTCCCTTAAATGGAA CCTCTGCTGCTGGGGGCCCTTGCCCACAGCACCGATTCTGACGTGGCCCAACCTGTCATGACTCAAGCTC ACCAGCAGGGGGCCTCCTGGTACCTCCCTTGCTCTTGGGCCTGCTTTTACTTCTGTTGGCCAGTTCTACC ACCACCCCTTATCTGGACCACTCTCTTTCCTGCATTCTGTAGAGTAGAAAGTACTGGACCCCCTAGAGGC CTTTGCTCACAGGCACACACTCCCTACCGCCACCATGGCTGGGGCCAAGCCCATGGGTATCTGCCCAACC CCTGGGCACTCCCTCCCCAGGATCGTGCAATAATAGTCAGAGGGTCCCTCCCAGGGGATGGGGCCATGGG TGCATGGCCCAGCTGTCTCCTCTCCATGCAAGTGCTGTTTTGGGCAGGAGTTGCCAGGCAGGGTGACATC GACAACCATGTACAAAGCCACCGCAGCTGCTGCCGCTCTGCCGCCTGTACAAAGGAAACTGAACCTTTTT CATATTCTAATAAATCAATGTGAGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6529306 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6529306 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17252 same embryo
	EMAGE:17255 same embryo
	EMAGE:17251 same embryo
	EMAGE:17253 same embryo
	EurExpress:euxassay_001642 same experiment
	MGI:4828287 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source