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EMAGE:17300

Rpl11 ribosomal protein L11 ( MGI:1914275)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300
euxassay_003729_01 euxassay_003729_02 euxassay_003729_03 euxassay_003729_04 euxassay_003729_05
EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300
euxassay_003729_06 euxassay_003729_07 euxassay_003729_08 euxassay_003729_09 euxassay_003729_10
EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300
euxassay_003729_11 euxassay_003729_12 euxassay_003729_13 euxassay_003729_14 euxassay_003729_15
EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300 EMAGE:17300
euxassay_003729_16 euxassay_003729_17 euxassay_003729_18 euxassay_003729_19 euxassay_003729_20
EMAGE:17300 EMAGE:17300
euxassay_003729_21 euxassay_003729_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17300Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17300_wholemount_strong.wlz
17300_wholemount_moderate.wlz
17300_wholemount_weak.wlz
17300_wholemount_possible.wlz
17300_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17300_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5722
Entity Detected:Rpl11, ribosomal protein L11 ( MGI:1914275)
Sequence:sense strand is shown

>T5722
CTCCACCATGGCGCAAGATCAAGGGGAAAAGGAGAACCCCATGCGGGAACTGCGCATCCGCAAGCTCTGC
CTCAATATCTGCGTCGGGGAGAGCGGAGACAGACTGACCCGGGCAGCCAAGGTGTTGGAGCAGCTCACAG
GCCAGACCCCGGTGTTCTCCAAAGCTAGATACACTGTCAGGTCCTTTGGCATCCGGAGAAATGAGAAGAT
TGCTGTTCACTGCACAGTCCGCGGAGCCAAGGCAGAGGAAATTCTGGAGAAAGGCCTGAAGGTGCGGGAG
TATGAGTTGCGGAAAAATAACTTCTCGGATACTGGAAACTTTGGTTTTGGAATTCAAGAACACATTGACC
TGGGCATCAAATACGACCCAAGCATTGGGATCTACGGCCTGGACTTCTATGTGGTGCTGGGTAGGCCAGG
GTTCAGCATCGCAGACAAGAAGCGCAGAACAGGCTGCATTGGGGCCAAACACAGAATCAGCAAGGAGGAG
GCCATGCGCTGGTTCCAGCAGAAGTACGATGGAATCATCCTTCCTGGAAAATAAACTTGATCCAAAAAGC
TAATAAAATTTTCTCAGAAATGCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:2647882 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647882 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17297 same embryo
 EMAGE:17299 same embryo
 EMAGE:17298 same embryo
 EMAGE:17296 same embryo
 EurExpress:euxassay_003729 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS