EMAGE mouse gene expression spatial database

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EMAGE:17318

Hoxa5 homeobox A5 ( MGI:96177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003722. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003722_01	euxassay_003722_02	euxassay_003722_03	euxassay_003722_04

euxassay_003722_05	euxassay_003722_06	euxassay_003722_07	euxassay_003722_08	euxassay_003722_09

euxassay_003722_10	euxassay_003722_11	euxassay_003722_12	euxassay_003722_13	euxassay_003722_14

euxassay_003722_15	euxassay_003722_16	euxassay_003722_17	euxassay_003722_18	euxassay_003722_19

euxassay_003722_20	euxassay_003722_21	euxassay_003722_22	euxassay_003722_23	euxassay_003722_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17318_wholemount_strong.wlz

17318_wholemount_moderate.wlz

17318_wholemount_weak.wlz

17318_wholemount_possible.wlz

17318_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17318_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

spinal cord

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

esophagus

moderate

regional

moderate expression: see section 13

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 09 15 16 17 18

lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

sternum

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6952
Entity Detected:	Hoxa5, homeobox A5 ( MGI:96177)
Sequence:	sense strand is shown >T6952 GGGTGCTATAGACGCACAAACGACCGCGAGCCACAAATCAAGCACACATATCAAAAAACAAATGAGCTCT TATTTTGTAAACTCATTTTGCGGTCGCTATCCAAATGGCCCGGACTACCAGTTGCATAATTATGGAGATC ATAGTTCCGTGAGCGAACAATTCAGGGACTCGGCGAGCATGCACTCCGGCAGGTACGGCTACGGCTACAA TGGCATGGATCTCAGCGTCGGCCGTTCGGGTTCCGGCCACTTTGGCTCCGGCGAGCGCGTCCGCAGCTAC GCGGCTGGGGCCAGTGCGGCGCCCGCCGAGCCCAGGTACAGCCAGCCGGCCACGTCCACGCACTCGCCAC CGCCCGACCCGCTGCCCTGCTCAGCGGTGGCCCCCTCGCCCGGCAGCGACAGCCACCACGGCGGGAAAAA CTCCCTGGGCAACTCCAGCGGCGCCTCGGCCAACGCCGGCAGCACCCACATCAGCAGCAGAGAGGGGGTT GGCACGGCGTCCGCAGCCGAGGAGGACGCCCCTGCCAGCAGCGAGCAGGCGGGCGCCCAGAGCGAGCCGA GCCCGGCGCCGCCCGCTCAGCCCCAGATCTACCCCTGGATGCGCAAGCTGCACATTAGTCACGACAATAT AGGTGGCCCAGAAGGCAAAAGGGCCCGGACGGCCTACACTCGCTACCAGACCCTGGAGCTGGAGAAAGAA TTCCACTTCAACCGCTACCTGACCCGCCGAAGAAGGATCGAAATAGCTCATGCCCTTTGCCTCTCCGAGA GACAAATTAAAATCTGGTTCCAAAACAGGAGGATGAAGTGGAAAAAAGATAATAAGCTGAAAAGCATGAG TATGGCCGCGGCAGGGGGGGCTTTCCGCCCCTGAGCATCTGAGCGGCCAAAGTACTGAGCAGTAGTAGCC GGGCAGCTCTCTGTAGTGTCAGTACTAAGGTGACTTTCTGAAACTCCCCTTGTGTTCCTTCTGTGAAGAA GCCCTGTTCTCGTTGCCCTAATTCATCTTTTAATCATGAGCCTGTTTATTGCCATTATAGCGCCTGTATA AGTAGATCTGCTTCTGTTCATCTCTTTGTCCTGAATGGCTTTGTCTTGAAAAAAAATAGATGTTTTAACT TATTTATATGAAGCAAGCTGTGTTACTTGAAGTAACTAAAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985274 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985274 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17320 same embryo
	EMAGE:17317 same embryo
	EMAGE:17319 same embryo
	EurExpress:euxassay_003722 same experiment
	MGI:4825418 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source