EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17329

Dnajc16 DnaJ (Hsp40) homolog, subfamily C, member 16 ( MGI:2442146)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003764. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003764_01	euxassay_003764_02	euxassay_003764_03	euxassay_003764_04

euxassay_003764_05	euxassay_003764_06	euxassay_003764_07	euxassay_003764_08	euxassay_003764_09

euxassay_003764_10	euxassay_003764_11	euxassay_003764_12	euxassay_003764_13	euxassay_003764_14

euxassay_003764_15	euxassay_003764_16	euxassay_003764_17	euxassay_003764_18	euxassay_003764_19

euxassay_003764_20	euxassay_003764_21	euxassay_003764_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17329_wholemount_strong.wlz

17329_wholemount_moderate.wlz

17329_wholemount_weak.wlz

17329_wholemount_possible.wlz

17329_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17329_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6981
Entity Detected:	Dnajc16, DnaJ (Hsp40) homolog, subfamily C, member 16 ( MGI:2442146)
Sequence:	sense strand is shown >T6981 GACCAGGCAGTACAACGTCAACCTCTATACCCCCACCATGCTCATCTTCAAAGAGCACATAAACAAGCCC GCTGATGTGATCCAGGCTCGGGGACTAAAGAAGCAGGTCATCGAGGACTTCATCGCCCAAAACAAGTACC TGCTGGCCTCCCGGCTCACCAGCCAGAGGTTGTTCCATGAACTCTGCCCCGTGAAGCGGTCTCACCGACA GAGAAAATACTGCGTGGTTTTACTGACAGCTGAGACTAACAAAGTGAGCAAACCCTTTGAAGCTTTCCTG TCATTTGCCCTGGCAAACACTCAAGACACGGTACGGTTCGTGCATGTCTACAGCAACCGACAGCAGGAGT TTGCCAGCACATTGCTACCGGACATGGAAGCTTTCCAGGGGAAGTCAGGGGTGTCCATCTTGGAAAGGAG GAACACAGCGGGGAGGGTGGTGTTCAAAACCCTGGAGGATCCCTGGACCGGCAGTGAGAGCGATAAGTTC GTCCTTTTGGCTTATCTTGACCAGCTGCGGAAGGATCCAGCATTTCTGTCTTCAGAAGCTGTGCTCCCTG ACCTGACTGACGAACTGGCTCCTGTATTTTTCCTCCGGTGGCTCTACTCTGTTTCTGACTACCTCTCGGA CTTCTGGGAGAGCCTGCTTCACAGTAACTGGCGGGAGATGATGCCCCTGCTGTCCCTCATCTTCTCGGCC CTCTTCATCCTCTTCGGCACGGTCATGGTCCAGGCTTTCAGTGACTCAAATGAGGAGCGAGAGTCGCACC CTGCAGACAAAGAGGAAGTCCCCGAGAAGGCTGGGAAGACTGAGCCAAGCTTCACCAAAGAGAGCAGCAG CAAGATTCCTAAGAAAGGCTTTGTGGAGGTGACTGAACTCACAGATGTGACGTACACCAGCAATTTGGTA CGCCTGAGGCCTGGTCACATGAATGTGGTCCTGATCCTGTCCAATTCCACTAAGACAAGCCTCCTGCAGA AGTTTGCTTTGGAAGTCTACACATTCACTGGGAGCAGCAGCCTCCACTTCTCCTTCCTGACACTGGACAA ACACCGGGAGTGGCTGGAGTATTTGCTAGAGTTTGCTCAGGATGCGGCCCCGATCCCAAACCAGTATGAC AAGCACTTTATGGAACGAGACTACACTGGCTACGTGCTGGCCCTGAACGGTCACAAGAAGTATTTCTGCC TTTTCAAGCCACTAAAGACAGTGGATGAGGAGACTGTGGCCTCCTGTGACCCTGACTCCTCCCGAGGGAA GCCTTCCTGTGGCCTTGGCCCCAAGCCCCTCAAAGGCAAGCTGAGCAAGTTGTCCTTGTGGATGGAGCGC CTGCTGGAAGGCTCCTTGCAGAGGTTCTACATCCCGTCCTGGCCTGAGCTGGACTGAGCGAGGCCTCCAG AGGATAGCGGGCCCCAGGAACAGGGATTTTAAGAGAGTCTCCATTGTAGATTTCTCTTCTAGAGCCATGG CTAGAAGACCTTTCCTCTGTCACCTAGGAGTAAAGCACGCAGGTTTGACCACTAGATGAACATCTTCCTT CCCCTTCGGGTTTTCTCCCCTGGATGAATGCCACACTACTTCAGTAGACTGCCCAGCCCCTCTTCTCTGC TGTCCCTATAACGTGCTCACACCCCTGTCCTTACACTGCAGAAACCCAGGGCTTGACCCTGAGCCGTGCC TGGACCAACCTCAGGATCTTCATGAGCCTGTCTCATGGGGTGGGTCACTCACCTCTCCCCCAGCCCCTGC AGCAGGCTCCTGCTGGCCCCTGCTTAGCCTCTGCCAAGGTGTGTGCTCTCAACACGGGAAGCCACCACGG CTTCTAGGTCCTCCGTCTTCAAAAGAGGCCTAGTGTGCAAGCAGTTTTCTATGTGGTTGTGCTTCTGGGA GCAAGCAGTGAGAAGTATATTTTGGGGGTATCCCCCACAACAGTCGGCCAAGCATGGTTTTGAGAAGAAA GGGGTGAGCAGGGGGTGAAGGTGCAGCATTCACATTTAGTCTCCCGACCGAGAGGGAGGGTGAGCACTGG CTGGAGGCGCAGAGCCCTGTGACCCTGCGTGCACTTCCCTGCCCGTCCCCTTCCCTCACCGCACTCTGTC AGCATCAGCACAGCCAGGGCGTCTGCCTTGCCAGCACTGGAGTGGCTGAGCTGCTGACCTGTCCCCAGAG GCCTTTTTGCTTCTGGGTTTTAGAGTTTGATACTAGGAGCCATATCCTGTGATCTTGTTCTCTGAACATA GATAAGCTGCTATAGAGCCAGTAGATTTAAGATGGCAGCACTTAGTCCTACCCGCCAAGAGCTAGGTGCA GGAGCCCTTCCTGCCTCGCTCTCCAGTAAAACGCCAGGTGTGTCTTCATACCAAGGAACCTGACCTCTCT GACAATTGCATTTTGAACATTGTTGTCCCCAAAGAGCTGAATCTTCAGGTCGTCTCTTGTGACTGTGAGC GGAGACTTGTCCGGACTCCTGTCAGTAGGCAGCTAGCTAGCATCCTCCTTCTGGCGTCCCTGCTTCACTG TTTCCTCCTTCCCTTATTGCTGCTTCCCGCTGCCAGATCACACCCATCAGCTCCAGTTCGCCTGGCTCCA CGCACCTTGAGCTCGGGGCCGGATCATGCTCGCCCGCTCAAGGGCATACCCTTGTTTTTAGAGCCAACCC ATGAGGGGAGTTTGGACAACTGTCACACATGATGTGTTCTACTGTCAAGCAAGTGCCCTTTTGTAAAGGG AAGGGCACAAATCAGGGACCGGACATTCACTCAGTGAGTATTCTCCAGTTGTAATTGCTTTGAACCAAGC ACCTCGGATCCTGACTACTTTAAACACTGAGCTCCAGTGGTAAGTGAGAAATTATTTGAACCCAGGAACT CTGTAGTTTTCAAAGAGTCTTTAGCTTTATTAAAACGTAAAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3989412 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3989412 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17332 same embryo
	EMAGE:17334 same embryo
	EMAGE:17331 same embryo
	EMAGE:17333 same embryo
	EMAGE:17330 same embryo
	EurExpress:euxassay_003764 same experiment
	MGI:4824353 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source