EMAGE mouse gene expression spatial database

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EMAGE:17351

Nudt4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 ( MGI:1918457)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003674. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003674_01	euxassay_003674_02	euxassay_003674_03	euxassay_003674_04

euxassay_003674_05	euxassay_003674_06	euxassay_003674_07	euxassay_003674_08	euxassay_003674_09

euxassay_003674_10	euxassay_003674_11	euxassay_003674_12	euxassay_003674_13	euxassay_003674_14

euxassay_003674_15	euxassay_003674_16	euxassay_003674_17	euxassay_003674_18	euxassay_003674_19

euxassay_003674_20	euxassay_003674_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17351_wholemount_strong.wlz

17351_wholemount_moderate.wlz

17351_wholemount_weak.wlz

17351_wholemount_possible.wlz

17351_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17351_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7023
Entity Detected:	Nudt4, nudix (nucleoside diphosphate linked moiety X)-type motif 4 ( MGI:1918457)
Sequence:	sense strand is shown >T7023 CCGGCGGGGTGGCGGCGGCCGGGTCCCCACGGTGGCGGCCGGAGCAGCGGCTGCAGGAGCCCGGCTCTAG GATGAAGTTCAAGCCCAACCAGACGCGGACGTACGACCGCGAGGGCTTCAAGAAGAGGGCGGCCTGCCTG TGCTTCCGCAGCGAGCAGGAGGACGAGGTGCTGCTGGTGAGTAGCAGTCGGTACCCAGACCAATGGATTG TCCCAGGAGGAGGGATGGAGCCGGAGGAGGAGCCTGGCGGTGCTGCTGTGAGGGAAGTGTATGAAGAGGC TGGAGTCAAAGGAAAACTAGGCAGACTCCTGGGAATATTTGAGAATCAAGACCGGAAGCACAGAACATAT GTTTATGTTCTGACCGTCACTGAAATATTGGAAGACTGGGAAGACTCCGTGAATATAGGAAGGAAGAGAG AGTGGTTCAAAGTAGAAGATGCAATCAAGGTTCTTCAGTGTCATAAGCCTGTCCACGCAGAGTATCTGGA AAAACTAAAGCTGGGCTGTTCTCCGACCAATGGAAATTCCTCGGTCCCTTCCCTCCCAGATAACAATGCC TTGTTTGTGACTGCTGCGCCGCCCTCTGGGGTGCCATCCAGTATAAGATAGAGGGTTGGGGTCCCTCCCC CATCATGTGATGCACATGTCACAAGGAGAGGCTCTTTCACTTCTAACACACCTGACTGCTGCTGGCAAAC TCTGTATTTGCTATGCAGGGATTTCAAATAATTTGCATGTATTTCATCTGCTTTCAAGTCTTTTAAAATA AATAACAAAATAGCAAAAAATATTTTAAGATGCCAAAGCCATGTGGAATTTTTTTTAAAGCCTTCATTGT ACCCCATTCCTTTGGTTTTCATTTTTATAACCTTTTTAACATTTTCACTTTTGTCCACTTAACATTCTAT GGTTTGTTTTGACAGATCAACTGGTTATTGGGTATATTTTTTGCCCAATTTTTTTTTCTTACAGTATCAA AATAGACACCAAATCTTTGTTGGGAATTAATGAAAAATTCTTGGTATTTAAAGAAATCTATCCACCCCAA AACTTAAAGAGAGTCATATTCTCCCTTTGTAAACTGCAGATTTGGGCCGTGTTTGTATGGGCCAGGAGCC TTTGACAAAAACACTTCAGCTTCCTATTTCTGAGAATTTGTTCACAGGCAGAATTATGTCACGGTCCATG TACTAGGACCGGTTCCCCTCTGCCACAGTGATTTGATATGAAAGTGTGCCGTCTTTTAGGGAAAACTGTC AGTACTTACACGTGCTGCATAGCTCATCCTGAAACTGGTTATAGAAATCTATTTAGCACGCTCAGCTGCT ATTTCCCTTCTTTGACATAGACTCTATTAGGGGCCACATTTCCCTCAAGGGTGAGTACTCTTTAAATATA TTTCCAGTTTCTGATTTAGAGACATTGTTCACCACTGGACTAGACTAACCTTGGATGCCGGTCAGTGACG ACTTTGTCACACTAAATAAAATGGCTGTTCTAGGCCTAAGACACTGTCTTGAGTGTCAGTGACGTGTCAG ATGAGTTAACTGTGGGGAAACTAGAACATTCGAGCCAAGAATTATTGTACCTCGCCTTTGTGCTGACTCA AAATTGGCCATCCCATGAAAAACTACCATGACTTCATGGAGGAAGTGAAGGGGGAGAAGAGGAAAAGATC TTTAGCCATTATTAACCTTTTGTAAATACTCTAACTGGCAAGCTTAAGACAGTGAAGACATTTGTTCTGT CGTTTGTTAACCTTTAAACTGTAATAGTGCTGCTGACAAGGGTCCTTGCCTGTAAGGAAAACTGGCACCG TTTTCATAATGAGATCTTGGGCCACCGATGCAGCAGAACTAAAAGCATTAGATTTCAAAGCAGAAGAACT CAGCCAGTAGTACTCATTCTAAATTATGTAGTGTAACCAAGCGTGATCGTCCAGACCTTTTATATTTTTC TTTGTACAGAGGTCATGTACTCTGGGTGTTTTAAAAGAAACATTTTAAAGAAACCCACAGATTGCCATTC TCTATCTTGTTTTCTCACGATTCTCTGCAATTCTTCCCTGTACTAAACACAACAGAGAAACTGGACTGTT TTGTATAGAATACTGCTTCCAGCAAATAAACCATCACAAGTGCTTGGGGGTTTGTATGTACACGATACTC TCAGAGGTAATAATGCATGAACCTATTTTATATGTAAAATATGTACAGTATTCATGTCAACCATCTCTTT AAAAGTTAGCCTATAAATATTGTTGTATAATTTCTTTGGTCAGAAACATTTGGACTAAGTCATGTCACCT GGGTCAGGATTTCTTTCAGTGCCATTTAGCAAACTGTCTTTAGTCTATGCAAATAGCAAAGATTTACAGA ATGCAACTGAATGCAGCTTCACTGTTAAATTTCACCTGAGGGCTCCTCATTCTTCTGGAACCCAGAACTT GAAAGATCTAAAGGAAATTGAATCTAGTAGAGCTCATGTCTACCAGCCCAGCCGCCTGCAGCGGGGAGAC TGCTGTTTGTGCTTATTTGTGCAGGCGCTGAATAAAACGGGGCAGCTAAATTCTCCAGTTCCATGTGCCT CCTAATGTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3994391 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3994391 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17350 same embryo
	EMAGE:17349 same embryo
	EurExpress:euxassay_003674 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source