EMAGE mouse gene expression spatial database

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EMAGE:17354

Slc25a44 solute carrier family 25, member 44 ( MGI:2444391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003808. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003808_01	euxassay_003808_02	euxassay_003808_03	euxassay_003808_04

euxassay_003808_05	euxassay_003808_06	euxassay_003808_07	euxassay_003808_08	euxassay_003808_09

euxassay_003808_10	euxassay_003808_11	euxassay_003808_12	euxassay_003808_13	euxassay_003808_14

euxassay_003808_15	euxassay_003808_16	euxassay_003808_17	euxassay_003808_18	euxassay_003808_19

euxassay_003808_20	euxassay_003808_21	euxassay_003808_22	euxassay_003808_23	euxassay_003808_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17354_wholemount_strong.wlz

17354_wholemount_moderate.wlz

17354_wholemount_weak.wlz

17354_wholemount_possible.wlz

17354_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17354_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7737
Entity Detected:	Slc25a44, solute carrier family 25, member 44 ( MGI:2444391)
Sequence:	sense strand is shown >T7737 TGCGAACCGAGTCTGGCTGTCGCTGGGACTGGAGGATAGCGCGGGGGCTGCGGTCTAGGAGTCCCAGTCT CAGACGCGGCTAAAGCGCTGGGGTCTGAGAAGGAAAGCGACCACGGTGCTTAGGTGTCTTGAAGGATCAG AGGTACCTAAGGTTCAACCTGTCGAGGAGGCAGTGGATATCTGTCTGTTCTGCGGGGCTGCATCGCCATG GGAAGCCGCTCTGCAGTTTTGGCAACGAAAGGCAAGTCCCCCGAGGCATCTCGTCCTGGCCGAGGAGCAT GAGAGACTGACAGCTAGAGAAGCCTGACGAGGGGTATGCCCGGGGACTCTGAATTGGCCGTGCAAGAGCC TGACAGAAGGTCTCCGGGCACAATGGAGGACAAACGGAACATCCAGATCATCGAGTGGGAGCACCTGGAC AAGAAGAAGTTCTATGTGTTTGGTGTTGCTATGACAATGATGATCAGGGTCAGCGTGTACCCGTTCACCC TCATCCGCACCCGCCTGCAGGTCCAGAAGGGCAAGAGCCTCTACCACGGGACCTTCGATGCCTTTGTCAA GATCCTGCGAGCAGATGGAGTGGCGGGCCTCTACCGGGGGTTCCTGGTCAACACCTTCACACTCATCTCC GGCCAGTGCTATGTCACCACTTACGAGCTCACCCGGAAGTTTGTGGCAGACTACAGCCAGAGTAACACAG TCAAATCACTCGTAGCCGGGGGCTCGGCCTCCCTTGTGGCCCAGAGCATCACGGTGCCGATCGATGTTGT CTCCCAACATCTGATGATGCAGCGGAAGGGTGAGAAAATGGGTCGCTTCCAAGTGCACGGGAACCTAGAG GGACAAGGGGTGATTGCCTTTGGCCAGACTAAGGACATCATCAGGCAGATCCTTCGGGCTGACGGTCTCC GAGGCTTCTACCGGGGCTATGTGGCCTCGCTGCTAACGTACATCCCAAACAGTGCTGTCTGGTGGCCCTT CTACCACTTCTATGCAGAGCAGCTGTCGCGCCTGTGCCCTCAGGAGTGCCCTCACATTGTCTTTCAAGCC ATCTCTGGGCCCCTGGCTGCAGCCACTGCCTCCATCCTCACCAATCCCATGGATGTCATCAGAACCCGTG TGCAGGTTGAAGGCAAGAGCTCCATCGTCCTGACCTTCAGGCAGCTGATGGCAGAGGAGGGGCCTTGGGG CCTCATGAAGGGCCTCTCGGCAAGAATCATCTCGGCTACACCCTCCACCATCGTCATCGTGGTGGGCTAC GAGAGCCTCAAGAAGCTCAGCCTGCGACCTGAGCTGGTGGACTCGAGACACTGGTAGAGAGCGGTGGATG GAGAGGCTGTGGTTTCACACATTACTCTGGGCCAGGGCAGAGCAGAGAGGATAACTGACCCTGTGAATAC CACCACCACACACCCATCCTGCTCCAGGTTGTCTGTCAGGGATAAGAGAGGAGACTTTGGACTGCTCCTT CTGCAGAGGTTCTGTGCCTGGCTTCCCTCATCCCCATCATTTGATTTCCCCTGCAGATCTGGGCCCCTCA CTCGGTCCTTATACTCAGTCATGGCCACAGGACCCCGTTAGCCCCTCTGGCACCCCTCTCTGACCTTCCC TCAGGCCTGGAGAAGAGCTGCTCCTCGTTTGCTTGTCCCCTGCTTCTTCTCGAGCCTCAGGCCAAGGCTC TGAGTAGCTGCCTGGGGTCTATTTATTTTTTTATGCCTGCTTATTTTAAATTTGGGACCTGAGTTGTTCA CTGCTTTGTCCTGCCTTTGGGGGGACAAATCGGAGCAGCACACACTCTGCAGGGCGTTCATCTTCTAGGG GAGTTACTAGGAGGGAGGAATGCTAAGGCTTCCTATGTGTAGCTGAACATCTGTGAATGAGCCAACTCAC CCTCATAGCTCAGTCTGCTGTCAGACCCCACTCCTTGGAGAAGTGGAAAACTGTGACACAGATCCCAATT CTTTATCCCCCTTCCCTGTCCCTCAGGCCCGGGAACCGCATGAGCTGCTGGCCTTCATTGGCCCAGGTGA CTACAGTAGAGGGGCATAGTTATAAGTGGGTCAGACTTACTAGAGAACACTGTCCTTGGAACTTTGCCCT GAAGCCAGGTTGGGAAAGAGCCACTGGCTTGGATGTGTGTTCCTCACTCTTATCTCCTCAGGCCAGCTGA GCCAAGCCCTGGAGGTACAACGGAGGTTCTGAGCCCGAGGCAGCAGTGCCACAGGCACACCTGCCACCCA GATGAATAGCAACAGGTGTCTCACAGTGGGACTCTGGAATATGAACTATCCTTTTTTTTCTTGTCCTTGG TTGTGAAGTATCATCCCAGTCATGGTACCCTGTGCTGGCAACAGAAAAAAAAAAAAAATACAGTCAGGAA GGCACCCAAGGCCAGGTCCACCTGGTTACCAGCCTCTTACTGCCAAAACCATTGATGCTTTCCTCAGGAG CGTCCCTCTTCCCTGCCTCTACTGAGCCTGTGTTGGTGGCAGAGAGGCACATCCAGGAATGGGAGGGAAG AAAGCAGGCAGATCTTGCTCTTCAGCCTGGGAGATGCTTGGGAAATGCCATGGTGCCAAATTGGAAGGTG CTAACTGAAGCCTTCATGTTTCTCACGGCTGCTCCACATTCTTCCATCCTGACTTGACCCAGTCTCCACA CACACACCTCCTACTACTCGCTGTAGTCTGTATTTGTTTCCTTCTGAGCCCTCTACTAATGTCTCAGCAT TTTGGTTTGTTTTTTGAGACAGGGTCTCATATATCCCAGGCTGGGTTTGAATTCATTATGTAGCAGAGGG TAGCCTAGAACTCTTCATCTTCCTCCACCTCCTAAGTTCCAGGTTTACAGGACGCCGCCAGGCCCCACTT CCTGCACTTTATTGAGAGAGCCCTTCAATCTTGCCCATAGTCATCTGAAGGGTGAAGCCCACAGATCATT TTAAATCCTTTCGCTCCTCACCTCTACCTTAAGACCTATCAATACAGTGGAGCAAGGGCCCCTCTCAGGC TTTCACTATCAAGACCTTTGTGATTGGACAGCTCTGCTCTTTGGTCTTTCCTAACGGGGCAGAGTGTGGA CATAGGTTCCTCCCACCTTTCATACCTTAGGTTACTCCTTCCCGACTTTTCTTGTCCGGTGTTTAAGCCT CTCCCTTTCTTGTCCTATCTCCCTTTCCTTCTTTCCCCTTGTGTTTGAAAGGACCTTGGATATAAAGGTA AAAGGCACCCCCGTGTCGGAACTCCTACTCTCCCAAGGTCAGGGTCTGGGATTGGCCTTGGTGAAGATGA GCCAGTGGGCGGCCATTTTTCCAAAACTGGAGACCAAAACAGCTAGAAATGTTGACCCATTGGTCAACAC ACAAATTAGAAACTCGGCAAGACAGGAGGGCTGGATAGAGGCACATGGGAAAGAATGCACATTCAGCATT AGCTAGAAATGGACTCGAGGAGATTAGCTGCGAATATTCACTGTTGCACTGTATGAGATCTTTGAAATGT AATTAAAAGTTTTTATTGAGCCCCTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310271 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310271 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17353 same embryo
	EMAGE:17352 same embryo
	EurExpress:euxassay_003808 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source