EMAGE mouse gene expression spatial database

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EMAGE:17376

Dusp18 dual specificity phosphatase 18 ( MGI:1922469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003743. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003743_01	euxassay_003743_02	euxassay_003743_03	euxassay_003743_04

euxassay_003743_05	euxassay_003743_06	euxassay_003743_07	euxassay_003743_08	euxassay_003743_09

euxassay_003743_10	euxassay_003743_11	euxassay_003743_12	euxassay_003743_13	euxassay_003743_14

euxassay_003743_15	euxassay_003743_16	euxassay_003743_17	euxassay_003743_18	euxassay_003743_19

euxassay_003743_20	euxassay_003743_21	euxassay_003743_22	euxassay_003743_23	euxassay_003743_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17376_wholemount_strong.wlz

17376_wholemount_moderate.wlz

17376_wholemount_weak.wlz

17376_wholemount_possible.wlz

17376_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17376_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 weak expression: see section 03 06 07 08 09 19 20 21 22

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 16

neural retina

moderate

regional

moderate expression: see section 02 03 04 24 weak expression: see section 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6993
Entity Detected:	Dusp18, dual specificity phosphatase 18 ( MGI:1922469)
Sequence:	sense strand is shown >T6993 GGCGGTGGGTGTAGCAATGGTCCTTCTGGCTACTTGGCGCATTTCTGTGAAGACAGGCAGCCCACCCAGC TTTTCCCAAACGCCTGGGAACTGCGTAGTCCTTCGAGGTACAGAATCAGTCGCGGGGTCCCCCTAAGCCC CGAACTGCCCATTGTTCCGCGAGGAGAAGCCGTTGCTACCAAGGTCCGGAATCATGAAGGGCCCTCTCTT TCTTGGAGAGGGTCGTCCGCGGCTCTGCCCTGAAAGATTTCGGGGGCACTGAGGGAAGGAGGTTTCAGTG GGCACAGCCTAGCAGAAAGATGCAAGCTTAATACGAGAAGTCATCCTCGACTTCCACTTTGATGACTTCA CCGTGGAGTGCCTTCCCAGTTCAGATCCCCCAGCCTTCGATCAGAGGCCTCTCGCAGATCACCAAGAGCC TGTTTATCAGCAATGGTGTGGCTGCCAACAACAAGCTCCTACTGTCCAGCAATCAGATCACCACAGTCAT CAACGTCTCAGTAGAGGTAGCAAACACCTTCTACGAGGATATCCAGTATGTGCAGGTGCCTGTGGTCGAT GCGCCCGTCGCACGCCTCTCCAATTTCTTCGATTCCGTTGCCGACCGTATCCATTCGGTGGAGATGCAGA AGGGCCGCACACTGTTGCATTGTGCTGCTGGGGTGAGCCGCTCAGCTGCGCTGTGCCTCGCCTACCTCAT GAAGTACCATGCCATGTCCCTTGTAGATGCCCACACTTGGACCAAGTCATGCAGGCCCATCATCCGGCCC AACAGTGGCTTTTGGGAGCAGCTCATCCATTACGAGTTGCAGCTCTTTGGCAAGAATACAATGCAGATGA TGGACTCGCCAATGGGACGGATCCCAGACATCTACGAGAAGGAGACCCGTTTGATGATCCCACTGTAAGC CAGCCCACCAGTAACTCCAGTACACCAAGGTCTGAACTTGAACTTCGACTTTTGTTGCTGCAGGAGCACA GCAGATGGTGCTTTTCATGACGCCAGAAAAAGTGTTGCTATAGCATTTAACCTCGCAGTCCAGCCAGGTT TGGTGGTGCATGCCTGTAACCTCAGATTGCTGAAGCAGAAAGATTACAAGGTGAGTTTGAGGCCAGCCTA GGCTACATATGAGACCCTATCTCAAAAACAAAATCAAGAACAGAAATTCCCAACAATTACTATCTTGTCA TCTGTGCTTTCAGAGAGTACATTCATTTCTTATAATATAGTGAAGGTAACTTTTCTGTGAGTGGTAGGGT GGAGCTGGCAGATGGTGCTGGGTCATAGGAACCAGTGCCCAGTGCATATGGCAAGCTGGGGTTCCTGCAG CCCTCCCGGAGATGACCAACCCAGAAACAAATCTGCCAAAATCCCACCTGGTTCTTTCAGAGGCAAATCC TGAAGCTTCCTGGTGCTCTTGGATGTCTCTCAATGTAAGTGCCAGCCTGCTAGATTAGGGGTCTTCAACC AGGGTTACAGAGACTGCCCCTTCTACCTTTACCACAGTGGCCCAATTCTGGTTTTCTAGATCAGATTCAC ACAGTGCTTGGATCCCAGTGGTGCCCAGATCAGCAATCCTACCTGTACACAGGCCTTAGTTTATACATGT TCTCAGAGCCTCTATGTGAGAGAGATGAAGAAATGGCTTGATCTCTGCTAGGCCTCATCCGAGAAGCCGC CTCCAGAGTTCTCACTTTGCAACCTGAGAGTCCTGACACAAGACCGCGTGCAGAATTGCAGACCATGAGA CTCAGGGTCCCAGGCTGGCTGACTGTTGAGGAGCTGTGAGGACTGTGAGGAACAAGTGAGTTGTCTCTAG CAAGCCTGAGATTCAGGGCTGCTCAGAAGCCCTTGGTGTTAGGGTCTGATTCAAGAAGGCTTGGTTTGGG TTGGTTTGGTTTAAGCCCTGCTCCCAGGTAGCCCAGGCTGTGTAGCCTTGAGCACCGGTCTGTCTCTTTG CCTGTACTTTAGGAATGCTGGGTTCACAGGTATGTGCCACCTTGTCCAGCCTAGACTTAGTGCTACAGTG TGTTTTTTTGTGGTATAAACTCACCCAAAGCATCTTGGGGGAGGAAAGGGTTTATTTGACTTACATGTTA CACTCTGTCATGAAGGAATGTGGCTTGCTCCCCGTGACTTGCTCAGTGCCACCTGCCCAGGTAGCCACAA CCATGGTGGACTAGACCCTCTCACATCAAGAAGCCCATCTCCGTGGAGGGTCCCTTGTCCCAGGTGACTC CAGTTACACTGACAAACACTGTAACAGTCTAATGGAGAGCCTGGTATGAGGTAGGCAGGAGAGGATGAAG CTAACTGCTGTTCTAGACTAATGTCATCCTTCATCAAGAGCAGCCCCATCCCTTCCCACCCCAGCCTTCA CCTGGTAGAAACACAGATTTTTTTTTTAACAAAGAACTTTATTTGAGCCTTTTTGTCATTAAAAGAAAAC ATTTGGAGGTGGAGGCTCGGTGACTATGAGAACTTACTGTTCTTCCCGAGGACCTGGGGTTTGATTCCCA GCACCGCATAGTGGCCCACAACTGTCTATGGTTCTAGTACCAAAGAATCTGTTGCCCTTTTCTCGTCTCC TAGAGTACCAGGCACACATGCAGTACAAATACATATGTTTAGGGAAAACACTCATCCCCAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3990751 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3990751 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17377 same embryo
	EMAGE:17375 same embryo
	EurExpress:euxassay_003743 same experiment
	MGI:4824415 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source