EMAGE mouse gene expression spatial database

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EMAGE:17407

Wdr46 WD repeat domain 46 ( MGI:1931871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003859. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003859_01	euxassay_003859_02	euxassay_003859_03	euxassay_003859_04

euxassay_003859_05	euxassay_003859_06	euxassay_003859_07	euxassay_003859_08	euxassay_003859_09

euxassay_003859_10	euxassay_003859_11	euxassay_003859_12	euxassay_003859_13	euxassay_003859_14

euxassay_003859_15	euxassay_003859_16	euxassay_003859_17	euxassay_003859_18	euxassay_003859_19

euxassay_003859_20	euxassay_003859_21	euxassay_003859_22	euxassay_003859_23	euxassay_003859_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17407_wholemount_strong.wlz

17407_wholemount_moderate.wlz

17407_wholemount_weak.wlz

17407_wholemount_possible.wlz

17407_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17407_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7796
Entity Detected:	Wdr46, WD repeat domain 46 ( MGI:1931871)
Sequence:	sense strand is shown >T7796 TGTTCGGTCTTGGAGATGGAGACAGCCCCCAAGCCGGGTAGGGGTGTCCCACCCAAGAGAGACAAGCCTC AGGCCAAGAGGAAGAAACCGCGGCGCTACTGGGAGGAGGAGACCACCCCGGCCGCCGTCGCCACCTCCCC GGGGCCGCCTCGGAAGAAGGCGAGGACTGGGGAGTCGCGACCCCCGAGGTCCAAGAGCGCGCACATCGCC CAGAAGTCCCGGTTCTCCAAGAAGCCCCCGATCTCAAAGACCGCTCCAGACTGGAAGAAGCCTCAGAGGA CGCTGTCGGGGGCTCAGGACCCGTTCCCAGGACCCGTCCCCGCCCCCCTGGAGGAGGCCCGGAAGTTTTG TCGAATTGACAAGTCCAAAACGCTCCCACATTCGAAGCCTAAAACTCAAAGCAAACTCGAGAAGGCTGAA GCGCAAGAGGAAGAGGCAAGCGTCAGAGCCGCCCGCGCTGAGCTGCTGCTTGCAGAGGAACCTGGGTTTC TGGTAGGAGAAGATGGGGAAGACACGGCAAAAATCCTGCAGACAGACATCGTGGAGGCTGTGGACATCGC CAGTGCTGCCAAGCACTTTGACCTGAATCTAAGACAGTTCGGACCCTACAGACTGAATTACTCTCGAACA GGGAGACACCTGGCTTTAGGAGGGCGTCGGGGTCACGTGGCTGCCCTCGACTGGGTGACCAAGAAGCTCA TGTGTGAGATCAACGTCATGGAAGCAGTACGGGACATCCACTTCCTCCATTCTGAGGCTCTGCTCGCAGT CGCTCAGAACCGCTGGCTTTATATCTACGACAACCAAGGCATCGAGCTTCACTGCATCCGCCGCTGTGAC CGAGTGACCCGGCTTGAGTTCCTCCCCTTCCACTTCCTCCTGGCCACCACTTCAGAGACAGGGTTTCTGA CCTACCTGGATGTGTCAGTGGGGAAGATCGTGACAGCTCTGAACGTGCGGGCGGGACGGCTCAGTGTCAT GGCTCAGAACCCCTACAATGCTGTTATCCACCTGGGACACAGTAATGGCACAGTGTCTTTATGGAGTCCA GCAGTGAAGGAGCCACTGGCGAAGATTCTGTGTCACCGTGGTGGGGTGCGTGCTGTGGCAGTAGATTCTA CAGGCACGTACATGGCCACCTCTGGTCTGGACCACCAGCTGAAGATCTTTGACTTGCGAGGGACATTCCA GCCCCTGAGTTCCCGGACCTTGCCCCAGGGAGCAGGGCACTTGGCCTTTTCCCAGCGGGGACTGTTAGTT GCGGGAATGGGTGATGTGGTCAACATATGGGCAGGGCAGGGCAAGGCCAGCCCACCCTCCCTGGAGCAGC CCTACCTCACCCACCGGCTCTCAGGCCACGTGCATGGGCTCCAGTTCTGCCCCTTTGAAGACGTGTTGGG GGTGGGGCACAGTGGAGGCTTCACCAGCATGCTGGTCCCTGGGGCTGCGGAGCCTAACTTTGATGGTTTG GAGAATAACCCATACCGGAGCCGGAAGCAGCGCCAGGAATGGGAGGTGAAGGCCCTGCTGGAGAAGGTAC CTGCAGAGCTCATTTGTTTGAACCCCCGAGCCCTGGCAGAGGTGGATGTGGTCACTCTGGAGCAGCAGAA GAAGGAGCGGATAGAGAGACTGGGCTATGATCCAGACGCCAAGGCTGCCTTTCAGCCCAAGGCCAAGCAG AAGGGCCGAAGCTCTACGGCGAGCCTAGTGAAGAGGAAGAAGAAGGTCATGGACCAGGAGCACAGGGACA AAGTCCGGCAGAGCCTTGAGCAGCAGCAGCAGAAGAAAAAGCAGGATATGGCCATGCCTCCTGGAGCCCG TCCCTCTGCACTGGATAGATTTGTACGACGAGCCAGAGAAGGCGGTCTCCAAGTTGACCCTTAGGGGCCA GAGTGCTCAGTGGGCAAAGGCACTTGATGCCAAACTTGAGTTCAGTTCCCAGAAAACACGTTTGGCAGGA GAAAACACACAAGTGTCTTTTTACCTTTCCATGCCCACATGGGGCAGCGCGCACACACACACACACACAC ACACACACACACACACACACATATATAAAGGGAACACAAAGATCCATAGGGAAATTGTCCCATGTGCCTT ACATCTTAACTGGGTAGGAGCAGACAGCTCCTTGGGTGTGTGGGCATTAAACAAGGTTTCTGGAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6315172 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6315172 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6089 same embryo
	EMAGE:17405 same embryo
	EMAGE:17409 same embryo
	EMAGE:17408 same embryo
	EurExpress:euxassay_003859 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source