EMAGE mouse gene expression spatial database

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EMAGE:17413

Zfp41 zinc finger protein 41 ( MGI:99186)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003860. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003860_01	euxassay_003860_02	euxassay_003860_03	euxassay_003860_04

euxassay_003860_05	euxassay_003860_06	euxassay_003860_07	euxassay_003860_08	euxassay_003860_09

euxassay_003860_10	euxassay_003860_11	euxassay_003860_12	euxassay_003860_13	euxassay_003860_14

euxassay_003860_15	euxassay_003860_16	euxassay_003860_17	euxassay_003860_18	euxassay_003860_19

euxassay_003860_20	euxassay_003860_21	euxassay_003860_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17413_wholemount_strong.wlz

17413_wholemount_moderate.wlz

17413_wholemount_weak.wlz

17413_wholemount_possible.wlz

17413_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17413_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7781
Entity Detected:	Zfp41, zinc finger protein 41 ( MGI:99186)
Sequence:	sense strand is shown >T7781 CGCCCGCCGGTCAGCCTGCTTACGGCCACGTTCCGCCGGCGTCTGTGCTCGGCACCCATCAGAAGCAAGA CGACCGTGGTTCTGTCTGCTTGATGCAGCTTCGTACGCAGGAGCTGTCCCTGACAGCGAAGTCCCAGAGA AGGAGCCAGGCAGCCACTGTCTGAAAACCCTCAGCCTTCCCTGGCCTCCAGGGGGGTTTCCTGCTTCCTA AGAGCAGCAATGGAAAAGCCTGCAACTAGGAAAAAGAAGAGTCAGGCCCCAAAGGAGGAGGCAGGTGCGC AAAAGGCCACTGTCAAGGGAGAGAAAACATCAAAGGGGAAAAAGGCAACCAAGAAGCCAAGAAAGCCCCG CAGACCCCGAAAAGAACCTGTCCTGAGCCCCGAGGACGAAGCACATATCTTTGATGCCTTCGATGCCTCG TTTAAAGATGACTTTGAGGGTGTCCCCGTGTTTGTCCCTTTTCAGAGGAAGAAGCCCTATGAGTGTGGTG AATGTGGACGGATCTTTAAACACAAGACAGATCACATTCGCCACCAGAGAGTTCACACTGGAGAGAAGCC CTTTAAGTGTGACCAGTGTGGGAAGACCTTCAGGCACAGCTCAGATGTCACCAAACATCAAAGAATTCAC ACCGGTGAGAAGCCCTTTAAATGTGGGGAGTGTGGAAAAGCCTTCAACTGTGGTTCTAACCTTCTAAAAC ACCAGAAAACGCACACTGGAGAGAAACCTTATGGCTGTGAGGAGTGTGGAAAATCCTTCGCCTACAGCTC CTGCCTCATCCGGCATCGGAAGCGTCATCCGAGGAAGAAGCACTGAGCAGGGCGGTCTACAGCTATGGAT CCTTGTCAAACACCTGATGATGGTCCAGAGGTCTTACGCAGTATGCTCATCCAGCCACCCATCTGTCATT GAGAGGAAAATCTACCCATCAGACATGAGAGCCCACAGAGGCTTTCTGCCCTTCGAGTCTTCACTTAGTA GTAACATGGTGCTTCCCGGAGCTTCCTGAAGCTCCAGCCATTTTCCAGACTACTTTATGTTACTCAGGGC CATCTTTGGAACAGTTTTGGGTCGCTGAGAACTTTGTAGAGCTAAAGAGACACCCCTGTTGGCAAGGGCC ACTGCCTTCCTTTGCTGCCTCTGTCTGCACCAACCACGGTGCTTTTGTCTCTTCTCTTGAAGACTTCTGA CTCTGTATGCTCAGCCCCATGCTGTCTGCTAGGGCGAGGAACCCTGAGGTGGATGACAGGCAGCATTCTG ACCAACTCTGAGGACAGCACCAAGTCCTGCTTGCTCATCTCTGTGCAGCTGACTTCAGTGATCCTAGAGT TTCACTCTTCTGGCTGAAACCACAGTCTTTGAGCTCAACCCTTGGGCCTAGAGCTGGGTTACACTGCTTT CTCTTGTCCCAGCCACATCGTAGACAAGTAGCCTGGATCCATCCAAAGCCTCAGCAGACCCTGGCACCCT GGCATAGTGGCAGGTTTCTTAGGCTAGGCCTTGTAGCTCCAGGGTGCCATCAGTAGCTTCTGCCCACTGC ACCTTGAAAAACAACTGTGGCCCCACCCCACAGCAGCCTGCCATTCCGGTCAATTCCACTTCCTGCTGGT CAGCAGGGCAAGACCACGTGACTCTGTCTACTGTCTGCCCCACGATGATGCTGTGTGAGGTCTGTGTTCT AAAGACAGCAAGTGACTGCTGACCAGGAGGGCTGCTAGAGCAGATCTCTCCAGTAAGAAGCTCAGAGAAC TCGGGAGTCAGTGTCTACCTCTACAGAGCACAGCAGAGACACTGGGCATGCTCCAGGGAGGCTTTCGTCG CCACAGAACCCACTGGCAACAGGAAGGCTGTAGGTGGGCGAGCTCAGGGAGGCCTTTTGGACGCCGTGTG GTGACTACAGGTCACTTTCACTTGTTCCCACCCTCAAGTTTAAGATGGCCACATCACAGCATTCATGAGC AACCATTAGTTCTGGCTCACTGAAGCCTGCCGAAGAGTTCCCGGCACCAGCTCTCTACATCAGTGAAAGA CACTGTGAACAGCCACCTGGGAACAAATGAAATGTTCAGAACCTTCTAGATGTCTTAAGATGTGTCCTGG GACAACTAAGAATACTCCAGCAAAGTATTGGGATTCTAATTATAGAAGCATGCTCAGCTGCAGGCAGAGA TGTCTTAGAAGTCCCAGGCAGTCCACTTATTAGCTGCCATGTCCAGTGGGCCTTGTCCTGCAGAGTTCCG AGCAGCCCAACTGCTGCTGAGCCTCACTTAGAACAGAGAGCACTACCAGGGCCTGACAACCAGCAAGTGA ATGCCAGCTCGGAGCTGTACTAAGATCCATTCCTTCAGCTCCCTCCCAGCCAAGAGAGAAGGCAGAAGCC ACCTCAAGGAGAGTTAGAGGGAGCTCTGGGCTGGGTCCAGGACTCTTCTGTTTGAGGAGAGCAGTGGGTT AGACCTGACTAGCTGCCTCCTTCTGTTTCTGAAAGTCTGTAGCTCCAGAGCACGTGCAGACTCCTTTCCT CCTGACCTTGGATCTGTCATCAAGACACTGACTTCCTGTGCCAATGAGTTGGGACATCAGGATGCCCTAA AAAGGAAGGCAGAACTGGGGCTCAAGGAAGAAAGGCTGTGTACCCTGACTCAAGGGACAGATGCTCTGTG CCTTCTAGGGGAGGCTCAGGGGAATGGGGTCACCTCTGGTGTGGACCCCAGCTACAGTGCAGACAAGAAA ACTGGCCTATGTGCCACATTTACCTTTAGGCCAAGCAATGGTGCCCACCTTCAGCTTACCCTTTCCAGTC CCTAGTGCCTGTGGAGTTGGGTGTTTGCTTTCTCCTGGTTCCTTCTCAAGGAAGACAAGTGGATTGACCT CCTGACTCAGATCCTGTAGGACTAATGCTTGCACTGGGCAGCTCCCAGAAACACCTAAGTGTGTCTGTTT CCCTGCATCTGGGCCTGCAGTCACTTGGCTTTGGACCTGCAGAGGGTCCCCAGCACCCGCATACTGGTAG CACTCAGCTGGCTTGTCCACATCTCACCTCAGCCACCTCATGATCTGGTGGAACCTCCTCTTTCTTGTTT CTGGCTGGTGCAGACACCCGTGTGTCCTAATACCCATCTGTACACTGCAGTGTGTGGTGCATTCCTCTTA AACAGCTGCAGCCATGGCTTGTTGTATTGTAGACGCTGTCCCCAGTCTGGTGTTTCTGTTTGACATTTGT GAATGCTTACGTCCGTGTAATACCCTTGGTGCTGTGTCAGTGTTGTGCACATTGGACTAGACATTCTGCT TTGTTGTGCACTTGGTGGCTTCCTTCCTATGTGACTCCTCTGCTCCATGGACAGTGGCACTGATGCTCCA CGTTGCCTTCCAGTTGCTGCCATGGTGCTGTGAGCAGATGCTGTTGGTGCTGCTCCCGGACATCTATTTT CTCATAAAAGTAAATGTGTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6314215 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314215 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17412 same embryo
	EMAGE:17410 same embryo
	EMAGE:17411 same embryo
	EurExpress:euxassay_003860 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source