EMAGE mouse gene expression spatial database

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EMAGE:17414

8430406I07Rik RIKEN cDNA 8430406I07 gene ( MGI:1921778)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003845. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003845_01	euxassay_003845_02	euxassay_003845_03	euxassay_003845_04

euxassay_003845_05	euxassay_003845_06	euxassay_003845_07	euxassay_003845_08	euxassay_003845_09

euxassay_003845_10	euxassay_003845_11	euxassay_003845_12	euxassay_003845_13	euxassay_003845_14

euxassay_003845_15	euxassay_003845_16	euxassay_003845_17	euxassay_003845_18	euxassay_003845_19

euxassay_003845_20	euxassay_003845_21	euxassay_003845_22	euxassay_003845_23	euxassay_003845_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17414_wholemount_strong.wlz

17414_wholemount_moderate.wlz

17414_wholemount_weak.wlz

17414_wholemount_possible.wlz

17414_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17414_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7801
Entity Detected:	8430406I07Rik, RIKEN cDNA 8430406I07 gene ( MGI:1921778)
Sequence:	sense strand is shown >T7801 GGGATGGCGCCTCACAGGTGCGACGGGAACGCCGTAGTGCATGCTGGGGCCGCCTCGCTTTGGGAAAGCG CGGGACCGATGAAAATACGAACAGAACACACTTTTAAACTTTCCAGACTAGAGAAAAGTTAGAGGGGCGA AGTCTAAAAATGAAACTGCCTCTGACCTTCTGCAGGCTGCTTAGCAGGTTAAACAGATTTTCTGTAAAAG CAAGCCCTCCTGTGAGTTTCTCCACTTTCTCTTATTTGTGTAGCCAGAAGAAGAAAAACTCTTACGAAGC AGTAGACCAAGCGAAGTACTCTCGTTTAGTACGCTCTGTCTTGTCCAGAGGCCCGGCCCAGACTCCAGAG TCGTTGTTCAAGGAAGATGATGTACTGTATGGACCAGTGAGTAAGCATAAGGCTGCAGAGCCGGAGCCAC AGGCCAGAGTCCCACAACACTGCTTTCCTATCTTCAATGAAGAGAGAACCGGGAAACCACACACAGATGC TTCTTCAAGCCCTTTGAAGATCCCTTTGCAAAGGAACTCGATACCCAGTGTGACCCGCATCCTTCAGCAG ACCATGCCACCTGAACAGAGCTTCTTTTTGGAGAGGTGGAAAGAGCGGATGGTTCTGGAGCTGGGAGAAG ACGGGTTTGCAGAATATACTTCAAATGTGTTTTTACAAGGCAAACAGTTCCATAAAGCCTTGGAAAGCAT ACTGTCACCCCAGGAGAACCTAACAGGGGGAGAAGAGCACCCCCAGTGTGGCTACATCGAAAGCATCCAG CATATTCTGACAGAAATCAGTGGTGTGCAAGCTCTGGAGAGTGCCGTCCAGCATGAGGCCTTGAAGTATG TAGGGCTGCTGGACTGTGTGGCTGAGTACCGGGGCAAGCTGTGTGTGATTGATTGGAAGACATCAGAAAA ACCAAAACCTTTAATTCGAAATACATATGACAACCCGCTGCAAGTTGTGGCGTACATGGGTGCCGTAAAC CATGATGCCCACTACAGTTTTCAGGTTCAGTGTGGATTAATTGTGGTGGCCTATAAGGATGGGTCCCCTG CGCACCCTCACTTCATGGATGAAGAGCTCTGTTCCAAGTATTGGGCCAAGTGGCTTCTCCGACTAGAAGA ATATACAGAAAAGCAAAAGAACCTGAGCGCTCCAGAGCCAGCGTAGAGGCCCGGGATTGCTTGGCGATGC TGCGCGGCGATCTCTGTTTGGGAGATGCACTGCTGTCCACTGAGTTTCTAAAGAGGAATGCGGGGAACTG AGGGCTACATTAACACAAGCTGAAGGTGTGGTGGGTTGGGATGTACCCACCGAGAAGCCAGAGAGTCTGG AGGTCAGCGCCTGACTCACCAGCTGCACGACCTCTGGCTGTTTCTCACCTGAGAGGAGGACACTGGGATT GGCAGCTGCCCAGGGACTTCTGGGCTCCCTTGCAAACCTCTGTGTCCCTGGCAGGATGCCCAGTCTTTGA ATTGAGATCAGAAGGCCAAATGAAGCATCATGTGCCACACATGTCCCTAGAGTTCTCAGGGATGGGAGGG GCCCTGCACCCTCAGGGTATACAGAGAGAGAGAGAGTGAGCAAGCAAGCACGTAGGCATCGCCACCACAG TTTCCTAGAAGGTGTTTTATACACTCTGTGTCATTAGGAAAAACTTATTTCAGCCTAAGGAAGCCTGGCT GCCTAGAGAGCTGGGGCAGGCAATACATCATTGGCTAGGTCCTTTTCCTTACCAGAGAAGCCCCAGCAGC AGGAAGCCTGAGTGTGGAAGTCACCGTCGCTAGGGTTTGGGTATCTATTTCTGGTGTCAGCAGGCCATGG ACTACAAAAAATACTGTTGTGGAACTTGTTAACAAAGAGGAACAATGGTAAAACTTTTTTTCTGTGTTTG ATAAGTAACAACAAAAAAAAAATCATTGGCTGTTCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6329248 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6329248 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17416 same embryo
	EMAGE:17415 same embryo
	EMAGE:17417 same embryo
	EurExpress:euxassay_003845 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source