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EMAGE:17437

Tmub1 transmembrane and ubiquitin-like domain containing 1 ( MGI:1923764)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437
"Pseudo-wholemount" of euxassay_003893. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003893_01 euxassay_003893_02 euxassay_003893_03 euxassay_003893_04
EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437
euxassay_003893_05 euxassay_003893_06 euxassay_003893_07 euxassay_003893_08 euxassay_003893_09
EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437
euxassay_003893_10 euxassay_003893_11 euxassay_003893_12 euxassay_003893_13 euxassay_003893_14
EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437 EMAGE:17437
euxassay_003893_15 euxassay_003893_16 euxassay_003893_17 euxassay_003893_18 euxassay_003893_19
EMAGE:17437 EMAGE:17437 EMAGE:17437
euxassay_003893_20 euxassay_003893_21 euxassay_003893_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17437Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17437_wholemount_strong.wlz
17437_wholemount_moderate.wlz
17437_wholemount_weak.wlz
17437_wholemount_possible.wlz
17437_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17437_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7265
Entity Detected:Tmub1, transmembrane and ubiquitin-like domain containing 1 ( MGI:1923764)
Sequence:sense strand is shown

>T7265
CCCGGAAGTGCCCGAGGAGCCGAGATGGAGCTGGTGGAGCTGGGCGCTCCTTAGCACCTCTGGCAGCAGC
ACGGGTGCCATGGCCTTGATTGAAGGCGTAGGCGATGAGGTGACTGTCCTTTTTGCGGTGCTTGCTTGCC
TTCTGGTGCTGGCCCTCGCCTGGGTCTCAACACATACCACTGAGAGTACAGACCCCCAACCACAGCCGCC
GGGGACCACAACACCAGCACAGCCCAGTGAAGCCATGTCAGCCTCTGACAGCATCAGAGAGGAGGCCCCA
GGGGCTGAGAGTCCCAGCCTGAGACACAGAGGTCCATCTGCACAGCCAGAGCCTGACACAGGGGTCACAG
CATCAACACCTCCAGACTCTCCGCAGGAACCCCTACTTCTACGGTTGAAATTTCTCAATGACTCTGAGCA
GGTAGCCAGGGCCTGGCCTCAGGACACCATTGGCTCCTTAAAAAGGACCCAGTTTCCAGGCCAGGAACAG
CAGGTTCGGCTCATCTACCAAGGTCAACTGCTAGGAGACGACACTCAGACACTAGGCAGTCTCCACCTGC
CCCCCAACTGCGTTCTCCACTGCCACGTGTCCACGAGAGTCGGTCCCCCACATCCTCCCTGCCCACCGGG
GTCAGAGCCCGGCCCCTCCGGGCTGGAAATCGGCAGCCTTCTGTTGCCCCTGCTGCTTCTGCTGCTGCTC
CTGCTCTGGTACTGCCAGATCCAGTACCGGCCCTTCTTTCCCCTGACCGCTACCCTGGGTCTGGCCGGCT
TCACCCTGCTCCTCAGTCTCCTGGCCTTTGCCATGTATCGGCCGTAGTGCCTCCACGGGCTTTTGGCAGT
GTAGCCAGCCCCTCTGGACCTCATTCCCCAAGGCGCAAAGGGAGCTGCTGTCTGCCCAGGCCCACTTCAC
CCGCTGCCTGTCTTTTCCCACTACCCTGGAGCCCAGCTTTGCGCCGCAGAGGACTTCGGGTGTTAGCGGA
GGCCGCTCCGTGTGATCTTCATAGCTCTAGGCCATCTCCTGGGGCTGCTGGACCTCAGCCTTCGCTGGCA
GTGGGCTCTTCTGGATCAGGCACTTGCTGTCGCTGCCTTGGCCGCCAGGGCACCCCAATGGGCCCATCTT
AGTGTGCTGACAGAGGATCTAGATACTTCTAGTTTCAAAGAGCTGCCTAGGCTGAATTGGAAACTGGTGG
ACTGGGAGACTGGTGAAGGGGAATCGAGAGGGGCAGAGTTGTTTTCTGGAATTTGTGCAGATTAAAGAAA
CTGTGACGTTTTCCTA
Notes:The probe template was PCR amplified from IMAGE:4164765 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4164765 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17436 same embryo
 EurExpress:euxassay_003893 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS