EMAGE mouse gene expression spatial database

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EMAGE:17446

Gmds GDP-mannose 4, 6-dehydratase ( MGI:1891112)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003883. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003883_01	euxassay_003883_02	euxassay_003883_03	euxassay_003883_04

euxassay_003883_05	euxassay_003883_06	euxassay_003883_07	euxassay_003883_08	euxassay_003883_09

euxassay_003883_10	euxassay_003883_11	euxassay_003883_12	euxassay_003883_13	euxassay_003883_14

euxassay_003883_15	euxassay_003883_16	euxassay_003883_17	euxassay_003883_18	euxassay_003883_19

euxassay_003883_20	euxassay_003883_21	euxassay_003883_22	euxassay_003883_23	euxassay_003883_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17446_wholemount_strong.wlz

17446_wholemount_moderate.wlz

17446_wholemount_weak.wlz

17446_wholemount_possible.wlz

17446_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17446_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

stomach

moderate

regional

moderate expression: see section 08 weak expression: see section 05 06 07

hindgut

strong

regional

strong expression: see section 13 14 15 16 17 moderate expression: see section 10 11 12

rectum

moderate

regional

moderate expression: see section 11

midgut

strong

regional

strong expression: see section 13 14 15 16 17 18 19 moderate expression: see section 10 11 12

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22

trachea

moderate

regional

moderate expression: see section 13

clavicle

moderate

regional

moderate expression: see section 08 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7269
Entity Detected:	Gmds, GDP-mannose 4, 6-dehydratase ( MGI:1891112)
Sequence:	sense strand is shown >T7269 GGCCCGCGGCTCGTTCCACTCCGCCTTCCTCTTGGCCGCACGCTCACCCGCCTGAGGCGACATGGCTCAA GCTCCCGCTAAGTGCCCGAGCTACCCGGGCTCCGGGGATGGCGAGATGGGCAAGCTCAGGAAGGTGGCTC TCATCACTGGCATCACCGGACAGGATGGTTCGTACTTGGCAGAATTCCTGTTGGAGAAAGGGTACGAGGT CCATGGAATAGTACGGCGATCTAGTTCATTTAATACAGGTCGAATTGAACATTTATATAAGAATCCTCAG GCTCATATTGAAGGAAACATGAAGTTGCACTATGGTGACCTCACTGACAGCACCTGCCTAGTGAAAATCA TCAATGAAGTCAAGCCTACAGAGATCTATAATCTTGGAGCCCAGAGCCATGTCAAGATCTCCTTTGACTT AGCTGAGTACACCGCAGATGTTGATGGCGTTGGCACCTTGCGGCTTCTGGATGCAATTAAAACTTGTGGC CTTATAAATTCTGTGAAGTTCTACCAGGCCTCAACAAGTGAACTTTATGGAAAAGTGCAGGAAATACCCC AGAAGGAGACCACACCTTTCTATCCGAGGTCACCCTATGGAGCAGCCAAACTCTATGCCTATTGGATTGT GGTGAATTTCCGTGAAGCTTATAATCTCTTTGCAGTGAATGGAATTCTCTTCAATCATGAGAGTCCCAGA AGAGGAGCTAATTTTGTTACTCGAAAAATTAGCCGGTCAGTAGCTAAGATTTACCTTGGACAACTGGAAT GTTTCAGCTTGGGAAATCTGGATGCCAAACGAGACTGGGGCCATGCCAAGGACTATGTAGAGGCTATGTG GCTCATGTTGCAGAATGATGAGCCAGAGGACTTTGTCATAGCTACTGGGGAAGTTCACAGTGTCCGTGAA TTTGTTGAAAAGTCATTCATGCACATCGGAAAAACCATTGTGTGGGAAGGAAAGAATGAAAATGAAGTGG GCAGATGTAAAGAGACCGGCAAAGTTCACGTGACTGTGGATCTGAAATACTACCGACCGACTGAAGTGGA CTTTCTGCAGGGAGACTGCTCCAAGGCTCAGCAGAAGCTAAACTGGAAGCCCCGCGTTGCCTTTGACGAG CTGGTGAGGGAGATGGTGCAGGCCGACGTGGAGCTCATGAGGACCAACCCCAACGCTTGAGCCCCTCTGC AGAGACTCGAGGGGCATGGTGACAGTGCAGAGCCAGGGAACCAGAGTCCAGTAACTCCTGCCAACCATGG ACCCTCGACCTCCTGTGCTGTCCCTGTATCCAGAGCTGGGCCACAGAGGTTTGTAGAGCCTGGGACAGGA CACACCAGAGCTAAGGCCGCATTGCTTTTGTCAAAGTCTCCTCCTCTGACTGGTTTCAGGAAATCAAGAA GTTTGAATCACATACTCATTTTACTTGAAATTATGTCACTAGACAACTTATATTTTGGAGTCTTGAGATT GTTTTTCTCTTTTCTTATTAAATAATCTTTCTATAACCCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4165509 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4165509 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17447 same embryo
	EMAGE:17445 same embryo
	EMAGE:17444 same embryo
	EMAGE:17448 same embryo
	EurExpress:euxassay_003883 same experiment
	MGI:4825122 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source