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EMAGE:17558

Timm8a1 translocase of inner mitochondrial membrane 8 homolog a1 (yeast) ( MGI:1353433)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558
"Pseudo-wholemount" of euxassay_005890. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005890_01 euxassay_005890_02 euxassay_005890_03 euxassay_005890_04
EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558
euxassay_005890_05 euxassay_005890_06 euxassay_005890_07 euxassay_005890_08 euxassay_005890_09
EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558
euxassay_005890_10 euxassay_005890_11 euxassay_005890_12 euxassay_005890_13 euxassay_005890_14
EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558 EMAGE:17558
euxassay_005890_15 euxassay_005890_16 euxassay_005890_17 euxassay_005890_18 euxassay_005890_19
EMAGE:17558 EMAGE:17558
euxassay_005890_20 euxassay_005890_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17558Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17558_wholemount_strong.wlz
17558_wholemount_moderate.wlz
17558_wholemount_weak.wlz
17558_wholemount_possible.wlz
17558_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17558_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8364
Entity Detected:Timm8a1, translocase of inner mitochondrial membrane 8 homolog a1 (yeast) ( MGI:1353433)
Sequence:sense strand is shown

>T8364
GGCACGAGGGTCGCTTCAGTGCTCGCCTCGGCCCGCGTGGTCGTGCCGGGATGGAGTCCTCCACGTCCTC
TTCGGGCTCGGCTCTGGGCGCCGTGGACCCTCAGTTGCAGCATTTCATCGAGGTGGAGACGCAGAAGCAG
CGCTTCCAGCAGCTGGTGCACCAGATGACGGAACTTTGCTGGGAGAAGTGCATGGATAAGCCTGGGCCTA
AGTTGGACAGTCGGGCTGAGGCCTGTTTTGTGAACTGCGTTGAACGCTTCATTGATACAAGCCAGTTCAT
CTTAAATCGACTGGAACAGACCCAGAAGTCCAAACCCGTCTTCTCAGAAAGCCTTTCTGACTGATCTCAG
CATTACCTCTTTGGAAAAGTAGTTCAAGAATTGAAGAGCTGCTGATGAGGTGGTTGAAGAAATGGCTATG
GGGGGGATTAAACTCCCAATTTTCTTTATCGTCTTGGGACATCTTGTCACCTGAGAATGAACAAAGACCA
ATTTTTATGTATGGGAGTTGAGAGTCATGTGTATTTGGTTGTGTTTTTAAAGCTAACCATGTGACAATAA
TTGACAGATAAATGAAAGTGTGAGATGCATATAACTGTATATGGGACTGCTTTCTCAAGGTCCCTTTACT
GCTTTATAAAATAATTTTGATAGGTGGACTGCTTTCTAGAATTTGATAGTGGGACCACATAGGGTAAATC
TTGATTTGTATGGGTTCATTTCTGATTTTTTTAGGGAGATCTTTGTGTCAGTCAAATTGGCAGTGGGGAA
AGATCCTGGCACTGTTTAACTGTGAGCTTTCTTTCATGTCTGACCTGGAGCTGAAAAAAAGTCCTATGGA
GTGTTCCTGCAATCCAACTTCTTAGGTTGTCTCTCCCCTATACTGTGACTTACTTAACTCGCAAATTGAA
AATATGGTCTCAAGGTAGAAGAGGAAGCCTGAGAATCCAAAGGGCTTTAGTCATAAAAGAATCTAGCTCA
TGAGTACTATTCTTATTACCAGACAGCCACGAGAAGAAGTTAACTTACTATGTATATCCAGATGCAAAAC
ATTCACAGATGTGCCTAAAGAAACCGCGACTTAGGCCAGTTCAGAACTAACAACTGTTTCAGGTAGAGGT
GCATGCCTGATGGTAGACATCGATTCCATTAACTGCATTCTTTTGGTCACTGAAATAAAAGCTCGCAAGA
TCAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3419065 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3419065 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17559 same embryo
 EMAGE:17560 same embryo
 EurExpress:euxassay_005890 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS