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EMAGE:17592

Leap2 liver-expressed antimicrobial peptide 2 ( MGI:2672795)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592
"Pseudo-wholemount" of euxassay_005925. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005925_01 euxassay_005925_02 euxassay_005925_03 euxassay_005925_04
EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592
euxassay_005925_05 euxassay_005925_06 euxassay_005925_07 euxassay_005925_08 euxassay_005925_09
EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592
euxassay_005925_10 euxassay_005925_11 euxassay_005925_12 euxassay_005925_13 euxassay_005925_14
EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592 EMAGE:17592
euxassay_005925_15 euxassay_005925_16 euxassay_005925_17 euxassay_005925_18 euxassay_005925_19
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euxassay_005925_20 euxassay_005925_21 euxassay_005925_22 euxassay_005925_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17592Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17592_wholemount_strong.wlz
17592_wholemount_moderate.wlz
17592_wholemount_weak.wlz
17592_wholemount_possible.wlz
17592_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17592_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T10055
Entity Detected:Leap2, liver-expressed antimicrobial peptide 2 ( MGI:2672795)
Sequence:sense strand is shown

>T10055
AGACTCCAACATCATTCTTTTGCCAAGATGCTACAGCTAAAACTCTTTGCAGTGCTCCTGACTTGCCTGC
TGCTGCTGGGTCAGGTCAATAGTTCCCCAGTACCAGAAGTGAGTTCAGCAAAGAGATCCCGGAGAATGAC
CCCATTTTGGAGAGGGGTTTCCCTCAGGCCCATTGGTGCCTCGTGCCGGGATGACTCTGAGTGTATCACA
AGACTATGCAGAAAAAGACGCTGTTCCCTAAGTGTGGCCCAGGAGTGATGTCCATACTGGGGAAGAGTCA
CCTCCAACAGTGATCTGTTCTGGGGAATATTATTAACGACATTTTGGTTGGAGATAGATAAGTGAAGTAG
TCTTTGTTTTTAATATTTAAAGACGGATACTTGCTTTAAACTGGTCTCCATTTCTTATTAGAATGTTGTT
GATTTGTACATAGACATAACTTTGTCAGAGTTTATTTTTCTATAAATACTATTAAATGTCTCAAATCTAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6749700 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6749700 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17591 same embryo
 EMAGE:17589 same embryo
 EMAGE:17590 same embryo
 EurExpress:euxassay_005925 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS