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EMAGE:17595

Mrps33 mitochondrial ribosomal protein S33 ( MGI:1338046)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595
"Pseudo-wholemount" of euxassay_005938. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005938_01 euxassay_005938_02 euxassay_005938_03 euxassay_005938_04
EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595
euxassay_005938_05 euxassay_005938_06 euxassay_005938_07 euxassay_005938_08 euxassay_005938_09
EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595
euxassay_005938_10 euxassay_005938_11 euxassay_005938_12 euxassay_005938_13 euxassay_005938_14
EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595
euxassay_005938_15 euxassay_005938_16 euxassay_005938_17 euxassay_005938_18 euxassay_005938_19
EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595 EMAGE:17595
euxassay_005938_20 euxassay_005938_21 euxassay_005938_22 euxassay_005938_23 euxassay_005938_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17595Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17595_wholemount_strong.wlz
17595_wholemount_moderate.wlz
17595_wholemount_weak.wlz
17595_wholemount_possible.wlz
17595_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17595_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8370
Entity Detected:Mrps33, mitochondrial ribosomal protein S33 ( MGI:1338046)
Sequence:sense strand is shown

>T8370
GGCACGAGGGTCAGGAGCAGGAATCCTGTTCCCTACTGGTGAAGAAATGTCTCCGCTTTCGGAGTATGCA
CTGCGCATGTCTCGTCTGAGTGCCCGGATCTTTGGTGAAGTGGCCAGGTCTACTGACTCAAAGTCCATGA
AAGTGGTGAGCCTGTTTAGCGAGCAGCCCTTGGCCAAGAAGAAAGAGACTTATGACTGGTATCCAAATCA
CAACACGTATTTTGCGCTCATGGGCAACCTCCGTTTCCTTGGCCTTTACAGAGATGAGCATCAGGACTTT
AAGGATGAGCAACGACGTCTAAAGAAGCTCCGTGGGAAGGGGAAACCGAGGAAAGGAGAAGGGAAGAGAG
CTACAAAAAAGAAATAGCATCAGCTCATCAAGAAAGCAAATGCCTTCCTCAGTGACTAAGAAAGTGCATC
TCATTATCTTCCCGAGTATTAGAGGAACATGACTTCCCTCAGTAGACGCCAACCCTGTGTTCACGTTTTA
CTTCAGTTAAGCTGTGACTGGTTTCTTGAAGATAATATTCTAATCCTTCAAAATTATAATGACCTTGGTT
TTGTAATCTGAAGCATACCTAATTCTATAATGGAGTGAATAAACTATTGTTGACTGAAAAAAAAAAAAAA
AAGAGAA
Notes:The probe template was PCR amplified from IMAGE:3466818 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3466818 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17594 same embryo
 EMAGE:17593 same embryo
 EMAGE:17596 same embryo
 EurExpress:euxassay_005938 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS