EMAGE mouse gene expression spatial database

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EMAGE:17638

Fbxo27 F-box protein 27 ( MGI:2685007)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005972. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005972_01	euxassay_005972_02	euxassay_005972_03	euxassay_005972_04

euxassay_005972_05	euxassay_005972_06	euxassay_005972_07	euxassay_005972_08	euxassay_005972_09

euxassay_005972_10	euxassay_005972_11	euxassay_005972_12	euxassay_005972_13	euxassay_005972_14

euxassay_005972_15	euxassay_005972_16	euxassay_005972_17	euxassay_005972_18	euxassay_005972_19

euxassay_005972_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17638_wholemount_strong.wlz

17638_wholemount_moderate.wlz

17638_wholemount_weak.wlz

17638_wholemount_possible.wlz

17638_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17638_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9530
Entity Detected:	Fbxo27, F-box protein 27 ( MGI:2685007)
Sequence:	sense strand is shown >T9530 TTCGGGGATGTTAGTCTCGGGGTGGACGGCTGCAAAGCCTGGCAGCAGAGGCTGCTAATCACTAACATCT AACCCCTCTTCTCCTGCAGTCCCAGCCAAGCGGGGCGCGGGGACCATGGGTGCCTGGATATCCAGGACCC GGGTCCCGACGCCGGAGCCCGACCCCCAGGAGGTCCTGGACCTGAGCCGTCTGCCCCCGGAGCTGCTCCT GCTGGTACTGAGCCACGTGCCCCCGCGCACGCTGCTGATGCACTGCCGCCGAGTGTGCCGCGCCTGGCGC GCCCTGGTAGATGGCCAGGCCCTATGGTTGCTGCTGCTGGCCCGTGACCACAGCGCAGCGGGCCGCGCCC TGCTGACACTAGCCCGCCGCTGTCTGCCCCCTGCCCACGAAGACACGCCCTGCCCGCTGGGCCAATTCTG CGCACTGAGACCGCTAGGACGCAACCTCATCAGCAACCCCTGTGGCCAAGAAGGCCTGCGCAAATGGATG GTGCGGCACGGTGGGGATGGATGGGTGGTGGAGAAGAACAGGAAACCTGTACCTGGGGCCCCTTCACAGA CCTGCTTCGTGACTTCCTTCAGCTGGTGTCGCAAGAAGCAGGTCGTGGACCTGGTGGAGAAGGGTCTGTG GCCAGAGCTGCTGGACAGTGGTGGTGTGGAGATTGCTGTCTCTGACTGGTGGGGAGCTCGACATGACAGT GGCTGTAAGTACCGTCTCTTTGTCACGCTTCTTGATGCCCACCAGAACGTCATAGATAAGTTCTCCGCTG TGCCAGATCCCATTGAACAGTGGAACAATGATATCTACCTGCAGGTCACCCACGTGTTCTCCGGCATCAG GAGGGGCATACGTTTTGTCTCTTTTGAACACTGGGGCCAGGACACACAGTTCTGGGCTGGCCACTATGGG GCCAGAGTGACGAACTCCAGTGTGATCATACGAGTCTGTCAGTCCTAGTCAAAAGGCCGCCATTTTGGCA AGACAATCTGAATGTGCCTTCCTGGAGCTGTCGGCTGGAATCCCTCATGAGTCAAGTACTGTACCTCCCT GGCACATGAAACTCTGGGATCCAGTGAAGGGTCCTGCTTGGTCTTGTTTTCAGAGTCTGTAACATTGAAG CCCTCCATCAGATCTACCTATTACTGCTGCTTTAGAGCATGAGTGCCTAGGTGCCTGGATGCATGTGTGC CCGGATGCATGGATGCCTGGGTTCCTAGATGCATAGATGCCTGGGTGCCTGGATGCATGGATGCCTGGGT GCCCGGATGCATGGATGCATGGGTGCCCGGATGCATGGGTGCATGCTGGTCTCAAGTCACAAGCTGCCTC TATTTCAGTTCGCTCACCTCCTTGCCCTGTCCCCAGTATTGATCCCCCACCCTTTTTTGAGACAGGGCAT CCCAGCTGGCGACAACCCTCCTTTCTGCCTTTGACACTCATGTGCTGGGACTACAGGCACACACCAGTAC CACTCAGTCTTGTGCAGTGCTGGGGATGAGCCCAGGGTTTCATGCATGCCAGCAAACATTCTACTATCTG AGCAATGTCCCCAGTGTCCCCTCTGGTCATGTCCTGTTTCTGCCTCAGGGATTGTCTCTGTTCCTTCGCT GAGGCCCTCTACTTAGGGCTGAACATAGGATTTATGGCTAGCAGAATTCCAGCTCCCTTGGGTAAGGAAC CAAAGCTCTCTTGGCTATAGCCAGCCTCCTCATTGAAGGGTGTTTTAGGGTATCTGCCCACAGATTTTCT CCAAACAATTGGGAAGTGCCTAGAGTCCAGGCCCCGATCATCAAAGATGTCTATATTGTATAATATTTAA AGTTATTTTTAATTTTCTCAAACAATATGAGAATATCTATCTGGCATTATCTATCTGCCAATCTCACTGT AATAAATTGATTATTTTGCCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30540609 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30540609 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17637 same embryo
	EMAGE:17635 same embryo
	EMAGE:17633 same embryo
	EMAGE:17634 same embryo
	EMAGE:17636 same embryo
	EurExpress:euxassay_005972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source