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EMAGE:17672

Cpsf1 cleavage and polyadenylation specific factor 1 ( MGI:2679722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672
"Pseudo-wholemount" of euxassay_006006. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006006_01 euxassay_006006_02 euxassay_006006_03 euxassay_006006_04
EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672
euxassay_006006_05 euxassay_006006_06 euxassay_006006_07 euxassay_006006_08 euxassay_006006_09
EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672
euxassay_006006_10 euxassay_006006_11 euxassay_006006_12 euxassay_006006_13 euxassay_006006_14
EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672 EMAGE:17672
euxassay_006006_15 euxassay_006006_16 euxassay_006006_17 euxassay_006006_18 euxassay_006006_19
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euxassay_006006_20 euxassay_006006_21 euxassay_006006_22 euxassay_006006_23 euxassay_006006_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17672Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17672_wholemount_strong.wlz
17672_wholemount_moderate.wlz
17672_wholemount_weak.wlz
17672_wholemount_possible.wlz
17672_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17672_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T224
Entity Detected:Cpsf1, cleavage and polyadenylation specific factor 1 ( MGI:2679722)
Sequence:sense strand is shown

>T224
GTGGCCGTCATCACCGNCTGGCACTACACAAGCCTCCACTGCATCATCAGTCCAAGGTGATTGCTCTCTG
CCTGTACCGCGATGTCAGTGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGATGAACTGGGT
GGCCGAAGTGGCTCCGAGGCAGAAGGCCTGGGCTCAGAAACGAGCCCCACAGTGGATGATGAGGAAGAGA
TGCTCTATGGGGACTCCAGTGCCCTCTTCAGTCCCAGCAAAGAGGAGGCCCGTAGGAGCAGCCAGCCACC
GGCTGATCGTGACCCTGCACCCTTCAAGGNAGACCCCACTCACTGGTGCCTGCTGGTGCGGGAGAATGGC
ACCATGGAGATCTACCAGCTCCCAGACTGGCGGCTGGTATTCCTAGTTAAGAACTTCCCCGNGGGGCAGC
GGGTTCTGGTGGACAGTTCATTTGGACAGCCCACTACACAAGGTGAAGTCCGAAAGGAANAANCCACACG
CCAGGGG
Notes:The probe template was PCR amplified from IMAGE:2649599 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2649599 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17675 same embryo
 EMAGE:17674 same embryo
 EMAGE:17673 same embryo
 EMAGE:17671 same embryo
 EurExpress:euxassay_006006 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS