EMAGE mouse gene expression spatial database

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EMAGE:17683

BC067068 cDNA sequence BC067068 ( MGI:3041259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006005. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006005_01	euxassay_006005_02	euxassay_006005_03	euxassay_006005_04

euxassay_006005_05	euxassay_006005_06	euxassay_006005_07	euxassay_006005_08	euxassay_006005_09

euxassay_006005_10	euxassay_006005_11	euxassay_006005_12	euxassay_006005_13	euxassay_006005_14

euxassay_006005_15	euxassay_006005_16	euxassay_006005_17	euxassay_006005_18	euxassay_006005_19

euxassay_006005_20	euxassay_006005_21	euxassay_006005_22	euxassay_006005_23	euxassay_006005_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17683_wholemount_strong.wlz

17683_wholemount_moderate.wlz

17683_wholemount_weak.wlz

17683_wholemount_possible.wlz

17683_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17683_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 07 08 09 15 16 17

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 20 21 22 23 24

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 12 13 14 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 14 15

cervical ganglion

moderate

regional

moderate expression: see section 09

thoracic ganglion

moderate

regional

moderate expression: see section 10 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 14 15 16

testis

moderate

regional

moderate expression: see section 06 07 08 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9575
Entity Detected:	BC067068, cDNA sequence BC067068 ( MGI:3041259)
Sequence:	sense strand is shown >T9575 CGCGGCCATGTTGGCGCCACCTGCAGCCCCGGAGGATGAGCGTCATGGCGGCGGCCTGCCCTCTGCCCAG GACCCCGGACCTGCCCACGCTGCACGACAAGTTACAGGGCTTGCTGAGCTTCCTCAGGGGCGCCCTGGCC ATTTCCAGCGCGCACACGGTGGACTTCTACACCAAGTCGGTCTGGCAGGAGCTGGTCGACCTGCCCCCTG AGAGCGTGCTGGCGGCGCTGAGGGAGTCGGCGGTGGAGGCCGAGCCGCGCGAGGCAGAGACGGGATCAGG TTTCACTGAGCTCCCCAAAATATTTTGTGAAACTTCTCAGAAGCTGTTGAGTGTGGAAGCTTTTGCCCGA ACTGCAAAGCACTATTCTGTGCAAAACTTGGGACTGTGCACTCCTTCTGAGCAGTTGCTCACAGCCCTCC AAGGAAATAAAAGGCAGAGAGTTGATGAAAATGTGAAGGCAATTGAGTTTATGAATACAAAAAAATCTCA TGAGGTTCAGGCAATGTCAGAGCTAATCTGCAGTATTGCTGATTACTGTGGCTTAAAGCAGATAATTGAC GTGGGTTCTGGTAAAGGCTATCTAAGCTCCTTTTTGTCCTTAAAATATGGTTTGAACGTTTATGGCATCG ATTCCTCAAACACTAACACTCACGGCGCGAAGGAGAGAAACAGGAAGTTGAAGAAGCATTGGAGCCTGTA TCACCCTCACTCAAGAGCAGACGCCAACGGCTGGGCATCAGAAAGGCCCAGAGAGTTGAAAGTGCCAAAG GGAGTGGAGTGTAAGGGGGACGCTGAGAGCGTGCAGAGGAGCAGGCTTGGGAATCCAGACCTGTCTGCCA CCGATGGTTTGCCTGATTTCTCAGGCTCTGCAATTTCAGTCATCAGAAAACAGCAAAAAAACGTGCTTGC TCAGCCAGCTGAGGAAGAAAATTTGTATTTTGAAGATGCCTTTTCTCTCATAGACTTTTTACCTGTTGAT GCCATCGAACCTACTTCATCACAGGTGCAGAACACAGAAAAGTCTGGACTGAGAAAAGAGAGAAGAAATA CGGCATCCAAGGCAAGGGACTCAAGCATATATTCACCACTAACCTCTTTCATCACTGCTGATTCACAATT GCATGACATTATTGAAGATCTGGAGGACTGTTTAATGGTGGGACTGCATACCTGTGGTGACCTTGCACCC AGTACTCTGCGGATATTTACATCCAAGGCTGAAGTCAAGGCAGTTTGCAGTGTGGGTTGTTGCTACCATC TCCTATCTGAGGAGTTTGAAAACCAGCATAAAGATAGATGTGCTAACGAAAACTGGGGGTTTCCAATGTG CCACTATCTGAAAGAGGAGAGATGGTGCTGCGGCCGTAATGCAAGAATGTCAGCATGTTTGGCATTGCAG CGGGTTGCAGTTGGCCAAGGGCTGCCTACTGAATCACTCTTCTATCGTGCTGTTCTTCAGAATATTATTA AGGATTATTATGGCATCAGCAAATGTGAACAGCACGTTGGTAAAATTTATTCCAAATGTTCCTCTTTTCT GGAGTATGTCAGAATGTCTCTAAAGAAGCTTGGATTAGATGAGTCCAAGGTCTCTGAAGAAATTATAATG GACTACTATGAGAATTATAAGCCTAGAATGAATGAGCTAGAGGCATTTAATATGTTGAAAGTTGTTCTGG CTCCTTGCATAGAAACACTGATTCTTCTTGATCGACTTTGTTACCTGAAGGAACAGGACGGTGTAGCCTG GTCTGCCCTCGTGAAGTTGTTTGACCCTGTGCAGTCGCCCAGATGCTACGCTGTCATTGCTCTGAAGAAG CAGTGTGATCTTGGCTGAAGCAGAGTGCTAGACAGCCTGGCTGGGGACTTACTGCTTTCTTAAACATTTA TATCCTGTTATGTAAACATTTTAAATCTATATTTTAAATAATAAAATATTGCCTAACAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30606425 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30606425 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17682 same embryo
	EMAGE:17684 same embryo
	EurExpress:euxassay_006005 same experiment
	MGI:4823434 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source