EMAGE mouse gene expression spatial database

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EMAGE:17767

Isl2 insulin related protein 2 (islet 2) ( MGI:109156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008929. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008929_01	euxassay_008929_02	euxassay_008929_03	euxassay_008929_04

euxassay_008929_05	euxassay_008929_06	euxassay_008929_07	euxassay_008929_08	euxassay_008929_09

euxassay_008929_10	euxassay_008929_11	euxassay_008929_12	euxassay_008929_13	euxassay_008929_14

euxassay_008929_15	euxassay_008929_16	euxassay_008929_17	euxassay_008929_18	euxassay_008929_19

euxassay_008929_20	euxassay_008929_21	euxassay_008929_22	euxassay_008929_23	euxassay_008929_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17767_wholemount_strong.wlz

17767_wholemount_moderate.wlz

17767_wholemount_weak.wlz

17767_wholemount_possible.wlz

17767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rest of cerebellum mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 14 15 16 17 18

midbrain mantle layer

strong

single cell

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 03 17 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 15 16 17 18 19

spinal cord floor plate

strong

regional

strong expression: see section 10 11 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35472
Entity Detected:	Isl2, insulin related protein 2 (islet 2) ( MGI:109156)
Sequence:	sense strand is shown >T35472 TGAGAGACGGGAAAACCTACTGCAAGCGGGACTACGTCAGGCTGTTCGGCATCAAGTGTGCCCAGTGCCA GGTGGGCTTCAGCAGCAGTGACCTGGTGATGCGGGCGCGGGACAGCGTGTACCACATCGAGTGCTTCCGC TGCTCCGTGTGCAGCCGCCAGCTGCTCCCTGGAGACGAGTTCTCGCTGCGGGAGCATGAGCTGCTCTGCC GAGCTGACCACGGCCTCCTGCTGGAGCGCGCTGCGGCTGGCAGCCCGCGCACCCCCGGCCCGCTCCCCGG CGCCCGCGGCCTGCATCTGCCAGACGCTGGGTCCGGACGACAGCCCTCACTGCGCACGCACGTCGACAAG CAGGCGGAGAAGACAACCCGGGTACGGACTGTGCTCAACGAGAAGCAACTGCATACCCTGCGGACGTGCT ACGCCGCCAATCCGCGGCCAGACGCGCTCATGAAAGAGCAGCTAGTAGAGATGACCGGCTTGAGCCCGCG GGTCATCCGCGTGTGGTTTCAGAACAAGCGTTGCAAGGACAAGAAGAAGTCCATTCTCATGAAGCAGCTA CAGCAGCAGCAACACAGTGACAAGGCGAGCTTCCAGGGACTGACTGGGACGCCTCTGGTGGCAGGCAGCC CCATCGGCCATGAGAACGCGGTGCAGGGCAGCGCAGTCGAGGTGCAGACGTACCAGCCGCCCTGGAAAGC ACTCAGCGAGTTTGCACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91356. Forward Primer - name:091356_F_cDNA_Isl2, sequence:TGAGAGACGGGAAAACCTACTG; Reverse Primer - name:091356_N_SP6_cDNA_Isl2, sequence:AGTGCAAACTCGCTGAGTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17769 same embryo
	EMAGE:17768 same embryo
	EMAGE:17770 same embryo
	EMAGE:17771 same embryo
	EurExpress:euxassay_008929 same experiment
	MGI:4825630 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source