EMAGE mouse gene expression spatial database

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EMAGE:17781

Msx3 homeobox, msh-like 3 ( MGI:106587)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008946. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008946_01	euxassay_008946_02	euxassay_008946_03	euxassay_008946_04

euxassay_008946_05	euxassay_008946_06	euxassay_008946_07	euxassay_008946_08	euxassay_008946_09

euxassay_008946_10	euxassay_008946_11	euxassay_008946_12	euxassay_008946_14	euxassay_008946_15

euxassay_008946_16	euxassay_008946_17	euxassay_008946_18	euxassay_008946_19	euxassay_008946_20

euxassay_008946_21	euxassay_008946_22	euxassay_008946_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17781_wholemount_strong.wlz

17781_wholemount_moderate.wlz

17781_wholemount_weak.wlz

17781_wholemount_possible.wlz

17781_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17781_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 17

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord mantle layer

strong

regional

strong expression: see section 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35490
Entity Detected:	Msx3, homeobox, msh-like 3 ( MGI:106587)
Sequence:	sense strand is shown >T35490 CTTAGATCATGGAATCAAGCCCTAACCTCTTGGTTAGGCTCAGGGTTGCGCACAGTTCTGCCTGCCGCTG ATGCTCCTGGTAACTTTTGACTTTTCATTTTAGTCTTCCGGGGGGGGGGGGGGGGGGGGTTTATACGGTG TTATACTTGATGACTGGAGCCTATTTATAGACATCTTACAAATACTTTAAACCTTCCTAAAACCTTGCCC ACATCAATCTGTTTTTCTGAAAGAAAACACACCGTCATATAGTCCTTGAAACAGACAAAAGCAGTTCTTC CTATTTCTTACAAAGCAGTCGACAAAGTATTTCTGGAGTGGGTGTGCTCCCTGTATTCACTCTGCCCGGA CACCGAGGACTTCAGAGAGGCTCGATTGAGCTGGCATAAGAAAACAAATTCCATAAGAAGAAATGAAAAT AAAAAATGAGATAATCGGACGGTTAGAAATTACTGTCTCTCCAACTGTTGTGACCAAATGGAAACTTTTA CGCTTTTTAGGTTTTATACCATTATTATTTTTTTTTTTAGAGTATATGTCGTTCAAGGAGCTAGAGGCAA ACGCTGGATGGACTTGGAAGTAGGGGAGGGGACTGTTGGCGCCATGCCCTAATGTAAGGGTGGCTGCTGC CCCCAGCATCTCCTGAGCTTCTTCGAAAAGAGCCTGCTGGGGGGAGACAGAGGGCTGAAGGCAGCAGGGA CCAACCTCTAGCCTCTTGCCAGCAGGTGTCCAGTCAATAAGAGAAGAAAGTGAGAGTTGAGCATCTCAGA TCTCCCATTGTGGGTGCGGTAGGAGGTGAGGCCTGTGAAGGTCCCAGAAGAGTTACTCTGAGTGCCGGCT GCCTCTGTAATTCTGGTGCTCCGTAGAAAAGCGGAGGACGTTGTTTAGGAATCCGGACTTGTACCTTTAT TCTACCTTTATCCGAGGAAAGAAAACAGGCCATCTGAGCTGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90226. Forward Primer - name:090226_F_cDNA_Msx3, sequence:CTTAGATCATGGAATCAAGCCC; Reverse Primer - name:090226_N_SP6_cDNA_Msx3, sequence:ACAGCTCAGATGGCCTGTTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17784 same embryo
	EMAGE:17782 same embryo
	EMAGE:17785 same embryo
	EMAGE:17783 same embryo
	EurExpress:euxassay_008946 same experiment
	MGI:4826476 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source