Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17844

Gclm glutamate-cysteine ligase, modifier subunit ( MGI:104995)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844
"Pseudo-wholemount" of euxassay_009046. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009046_01 euxassay_009046_02 euxassay_009046_03 euxassay_009046_04
EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844
euxassay_009046_05 euxassay_009046_06 euxassay_009046_07 euxassay_009046_08 euxassay_009046_09
EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844
euxassay_009046_10 euxassay_009046_11 euxassay_009046_12 euxassay_009046_13 euxassay_009046_14
EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844
euxassay_009046_15 euxassay_009046_16 euxassay_009046_17 euxassay_009046_18 euxassay_009046_19
EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844 EMAGE:17844
euxassay_009046_20 euxassay_009046_21 euxassay_009046_22 euxassay_009046_23 euxassay_009046_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17844Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17844_wholemount_strong.wlz
17844_wholemount_moderate.wlz
17844_wholemount_weak.wlz
17844_wholemount_possible.wlz
17844_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17844_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver left lobe
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13
liver right lobe
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36422
Entity Detected:Gclm, glutamate-cysteine ligase, modifier subunit ( MGI:104995)
Sequence:sense strand is shown

>T36422
GTGGAGAAGATAAATCCCGATGAAAGAGAAGAAATGAAAGTTTCTGCAAAACTGTTCATTGTAGGATCGA
ATTCTTCATCATCAACTAGAAGTGCAGTTGACATGGCATGCTCCGTCCTTGGAGTTGCACAGCTGGACTC
TGTGATCATGGCTTCGCCTCCGATTGAAGATGGAGTTAATCTTTCCTTGGAGCATTTACAGCCTTACTGG
GAGGAATTAGAGAACTTAGTTCAGAGCAAGAAAATTGTTGCTATAGGCACCTCTGATCTAGACAAGACAC
AGTTGGAGCAGCTGTATCAGTGGGCACAGGTAAAACCCAATAGTAACCAAGTTAACCTGGCCTCCTGCTG
TGTGATGCCACCAGATTTGACTGCCTTTGCTAAACAATTTGACATACAGCTACTGACTCACAATGACCCG
AAAGAACTGCTCTCTGAGGCAAGTTTCCAGGAAGCTCTTCAAGAAAGCATCCCTGACATTGAAGCCCAGG
ATTGGGTGCCGCTGTGGCTGCTGAGGTACTCGGTCATCGTGAAGAGCAGGGGAATCATCAAGTCTAAAGG
CTACATTCTGCAAGCCAAAAGGAGGGGCTCTTAACTCCAGCTCACACCCACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95859. Forward Primer - name:095859_F_cDNA_Gclm, sequence:GTGGAGAAGATAAATCCCGATG; Reverse Primer - name:095859_N_SP6_cDNA_Gclm, sequence:CTGTGGGTGTGAGCTGGAGTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17843 same embryo
 EMAGE:17842 same embryo
 EMAGE:17841 same embryo
 EMAGE:17840 same embryo
 EurExpress:euxassay_009046 same experiment
 MGI:4825024 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS