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EMAGE:17905

Stac src homology three (SH3) and cysteine rich domain ( MGI:1201400)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905
"Pseudo-wholemount" of euxassay_009608. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009608_01 euxassay_009608_02 euxassay_009608_03 euxassay_009608_04
EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905
euxassay_009608_05 euxassay_009608_06 euxassay_009608_07 euxassay_009608_08 euxassay_009608_09
EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905
euxassay_009608_10 euxassay_009608_11 euxassay_009608_12 euxassay_009608_13 euxassay_009608_14
EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905 EMAGE:17905
euxassay_009608_15 euxassay_009608_16 euxassay_009608_17 euxassay_009608_18 euxassay_009608_19
EMAGE:17905
euxassay_009608_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17905Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17905_wholemount_strong.wlz
17905_wholemount_moderate.wlz
17905_wholemount_weak.wlz
17905_wholemount_possible.wlz
17905_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17905_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37083
Entity Detected:Stac, src homology three (SH3) and cysteine rich domain ( MGI:1201400)
Sequence:sense strand is shown

>T37083
AATAAAGTGGACCCCGTGTATGAGGCCCTCCGGTTCGGCACGTCCCTGGCCCAGAGAACGAAGAAGGGCG
GCTCAGGCAGTGGTTCTGACTCACCGCCCAGAACCTCGACTTCAGAGCTTGTAGACGTCCCTGAAGAGGC
CGATGGGCCAGGAGATGGATCTGACATGAGGACACGGAGCAACAGTGTGTTTACATATCCAGAAAACGGT
ATGGATGACTTCAGAGATCAAATGAAGACCACAAACCACCAGGGACCTCTTTCCAAAGACCCATTACAGA
TGAACACCTACGTTGCCTTGTACAGATTTATACCACAGGAGAATGAAGACTTGGAAATGAGGCCAGGAGA
CATGATCACTCTCCTAGAAGACTCCAATGAAGACTGGTGGAAAGGAAAGATTCAGGACAGGGTTGGTTTC
TTTCCAGCCAACTTTGTTCAGAGAGTAGAAGAGCATGAGAAGATTTATAGGTGTGTTCGAACCTTCATTG
GCTGCAAGGACCAGGGGCAGATAACACTGAAAGAGAACCAGATATGCGTGACCTCTGAGGAAGAACAGGA
CGGCTTCATCAGAGTCCTCAGTGGGAAGAAGAGAGGCCTCGTCCCACTGGATGTACTGGTAGACGTGTGA
CTGTAGCAGTCCCTTCCCGAGCAGGAGGCCAGCGCTGCTGATCTCTCTGTGTCCACCCAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74847. Forward Primer - name:074847_F_cDNA_Stac, sequence:AATAAAGTGGACCCCGTGTATG; Reverse Primer - name:074847_N_SP6_cDNA_Stac, sequence:TCTGGGTGGACACAGAGAGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17907 same embryo
 EMAGE:17909 same embryo
 EMAGE:17908 same embryo
 EMAGE:17906 same embryo
 EurExpress:euxassay_009608 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS