EMAGE mouse gene expression spatial database

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EMAGE:17932

Dock10 dedicator of cytokinesis 10 ( MGI:2146320)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011695. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011695_01	euxassay_011695_02	euxassay_011695_03	euxassay_011695_04

euxassay_011695_05	euxassay_011695_06	euxassay_011695_07	euxassay_011695_08	euxassay_011695_09

euxassay_011695_10	euxassay_011695_11	euxassay_011695_12	euxassay_011695_13	euxassay_011695_14

euxassay_011695_15	euxassay_011695_16	euxassay_011695_17	euxassay_011695_18	euxassay_011695_19

euxassay_011695_20	euxassay_011695_21	euxassay_011695_22	euxassay_011695_23	euxassay_011695_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17932_wholemount_strong.wlz

17932_wholemount_moderate.wlz

17932_wholemount_weak.wlz

17932_wholemount_possible.wlz

17932_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17932_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 07 08 15 16

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

thyroid gland

weak

regional

weak expression: see section 09 10 13

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 14 15 weak expression: see section 07

facial vii ganglion

weak

regional

weak expression: see section 04 05 06 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 16 weak expression: see section 06 07

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17

trigeminal v nerve

moderate

regional

moderate expression: see section 09 16

dorsal root ganglion

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37682
Entity Detected:	Dock10, dedicator of cytokinesis 10 ( MGI:2146320)
Sequence:	sense strand is shown >T37682 ATTACTAACAACTCCAGGCGGAGGAAGCATGTTCTCTATGGGATGGCCAGCCTTTCTGAGCATCACCCCA AACATTAAAGAAGAAGGAGCAATGAAAGAGGATTCTGGAATGCAAGACACCCCGTACAATGAGAACATCC TAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGT CAATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGAC ATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATA GAGTGGCGTTTTATGGGCAGGGATTCTTTGAGGAGGAGGAAGGTAAAGAGTATATCTACAAAGAGCCTAA GCTGACAGGGCTCTCGGAGATCTCCCAAAGGCTTCTCAAGCTCTATGCAGACAAATTTGGAGCAGACAAT GTGAAAATAATTCAAGATTCCAACAAGGTAAACCCCAAGGATCTGGACCCCAAATATGCCTATATTCAGG TGACCTATGTCACACCATTCTTTGAAGAAAAGGAAATCGAGGACCGAAAGACAGACTTTGAAATGCATCA CAACATCAATCGCTTTGTCTTTGAGACACCCTTCACTCTGTCAGGCAAGAAGCACGGAGGAGTGGCTGAG CAGTGCAAGCGGAGGACAGTCCTGACCACAAGCCACTTGTTCCCCTACGTAAAGAAGAGGATCCAGGTCA TCAGCCAATCAAGCACAGAGCTGAATCCTATCGAGGTGGCAATTGATGAGATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75762. Forward Primer - name:075762_F_cDNA_Dock10, sequence:ATTACTAACAACTCCAGGCGGA; Reverse Primer - name:075762_N_SP6_cDNA_Dock10, sequence:CATCTCATCAATTGCCACCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17934 same embryo
	EMAGE:17933 same embryo
	EMAGE:17936 same embryo
	EMAGE:17935 same embryo
	EurExpress:euxassay_011695 same experiment
	MGI:4824369 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source