EMAGE mouse gene expression spatial database

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EMAGE:17977

Hexb hexosaminidase B ( MGI:96074)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011735. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011735_01	euxassay_011735_02	euxassay_011735_03	euxassay_011735_04

euxassay_011735_05	euxassay_011735_06	euxassay_011735_07	euxassay_011735_08	euxassay_011735_09

euxassay_011735_10	euxassay_011735_11	euxassay_011735_12	euxassay_011735_13	euxassay_011735_14

euxassay_011735_15	euxassay_011735_16	euxassay_011735_17	euxassay_011735_18	euxassay_011735_19

euxassay_011735_20	euxassay_011735_21	euxassay_011735_22	euxassay_011735_23	euxassay_011735_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17977_wholemount_strong.wlz

17977_wholemount_moderate.wlz

17977_wholemount_weak.wlz

17977_wholemount_possible.wlz

17977_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17977_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

strong

single cell

strong expression: see section 08 09 10 11 12 13 14 15

sternum

strong

regional

strong expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37736
Entity Detected:	Hexb, hexosaminidase B ( MGI:96074)
Sequence:	sense strand is shown >T37736 ACGACCAGTCTTTCCCTTATCAGAGTACCACTTTTCCTGAGCTAAGCAATAAGGGAAGCTACTCTTTGTC TCATGTCTATACACCAAACGATGTCCGGATGGTGCTGGAGTACGCCCGGCTCCGAGGGATTCGAGTCATA CCAGAATTTGATACCCCTGGCCATACACAGTCTTGGGGCAAAGGACAGAAAAACCTTCTAACTCCATGTT ACAATCAAAAAACTAAAACTCAAGTGTTTGGGCCTGTAGACCCAACTGTAAACACAACGTATGCATTCTT TAACACATTTTTCAAAGAAATCAGCAGTGTGTTTCCAGATCAGTTCATCCACTTGGGAGGAGATGAAGTA GAATTTCAATGTTGGGCATCAAATCCAAACATCCAAGGTTTCATGAAGAGAAAGGGCTTTGGCAGCGATT TTAGAAGACTAGAATCCTTTTATATTAAAAAGATTTTGGAAATTATTTCATCCTTAAAGAAGAACTCCAT TGTTTGGCAAGAAGTTTTTGATGATAAGGTGGAGCTTCAGCCGGGCACAGTAGTCGAAGTGTGGAAGAGT GAGCATTATTCATATGAGCTAAAGCAAGTCACAGGCTCTGGCTTCCCTGCCATCCTTTCTGCTCCTTGGT ACTTAGACCTGATCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64070. Forward Primer - name:064070_F_cDNA_Hexb, sequence:ACGACCAGTCTTTCCCTTATCA; Reverse Primer - name:064070_N_SP6_cDNA_Hexb, sequence:TGATCAGGTCTAAGTACCAAGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17975 same embryo
	EMAGE:17973 same embryo
	EMAGE:17976 same embryo
	EMAGE:17974 same embryo
	EurExpress:euxassay_011735 same experiment
	MGI:4825363 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source