EMAGE mouse gene expression spatial database

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EMAGE:18024

Tll2 tolloid-like 2 ( MGI:1346044)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011790. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011790_01	euxassay_011790_02	euxassay_011790_03	euxassay_011790_04

euxassay_011790_05	euxassay_011790_06	euxassay_011790_07	euxassay_011790_08	euxassay_011790_09

euxassay_011790_10	euxassay_011790_11	euxassay_011790_12	euxassay_011790_13	euxassay_011790_14

euxassay_011790_15	euxassay_011790_16	euxassay_011790_17	euxassay_011790_18	euxassay_011790_19

euxassay_011790_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18024_wholemount_strong.wlz

18024_wholemount_moderate.wlz

18024_wholemount_weak.wlz

18024_wholemount_possible.wlz

18024_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18024_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

weak

spotted

weak expression: see section 09 12 13

diencephalon floor plate

weak

regional

weak expression: see section 12 13

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37139
Entity Detected:	Tll2, tolloid-like 2 ( MGI:1346044)
Sequence:	sense strand is shown >T37139 GCAGAGAGACTGAGGGTGTTTTAAACACTTGCGAGTGAGCAGCCTCCCATGTACAGTGTTTTTTCTCCAC AACAAAAACCCAAACTATGTTCTTGAAACTCTATATCTGGGTGACAGTACATGCCTTTGGCCAACGGGAG GAGAGAGAGGGGCCGTTGGTTCTGGCTGTGGCGTTATCAGTCGCAGCTTCTGGAGGAGGTCAAAGTTTGA TGTTAGCGACCAAACAGGATGACTTCTTCACATTTGTCTCTAAGCTTTGCTCCTGTGGCTCCGAAGGCCA GCCTGGGGCAAAAGGACAGCTTAGGGCAAAAGACTGCCTCTCTCTCAACTCTGGCCCGGCTTCCATAGAT GGCATGAGCCCAGTGTGCTGGTGACTTGGCTGCCCTCTGACTACTGGGCATGGAGGATTATAGATTGGCA GCGGCTTCGCTGATACTCGCAGGTGTGGCTGAACGCTTCTGTGCAGTTCTACCAGAGTCAAGCCAATCAA GAAACCGGAGACCGTGGACACGAGAGTTAAGGTGGCATCCCAGGGCACAGGGAGGGGGCTCAACCTGGGA ACCACATAATGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74602. Forward Primer - name:074602_F_cDNA_Tll2, sequence:GCAGAGAGACTGAGGGTGTTTT; Reverse Primer - name:074602_N_SP6_cDNA_Tll2, sequence:CCATTATGTGGTTCCCAGGTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18028 same embryo
	EMAGE:18026 same embryo
	EMAGE:18023 same embryo
	EMAGE:18027 same embryo
	EMAGE:18025 same embryo
	EurExpress:euxassay_011790 same experiment
	MGI:4828738 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source