EMAGE mouse gene expression spatial database

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EMAGE:18029

Tmc3 transmembrane channel-like gene family 3 ( MGI:2669033)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011796. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011796_01	euxassay_011796_02	euxassay_011796_03	euxassay_011796_04

euxassay_011796_05	euxassay_011796_06	euxassay_011796_07	euxassay_011796_08	euxassay_011796_09

euxassay_011796_10	euxassay_011796_11	euxassay_011796_12	euxassay_011796_13	euxassay_011796_14

euxassay_011796_15	euxassay_011796_16	euxassay_011796_17	euxassay_011796_18	euxassay_011796_19

euxassay_011796_20	euxassay_011796_21	euxassay_011796_22	euxassay_011796_23	euxassay_011796_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18029_wholemount_strong.wlz

18029_wholemount_moderate.wlz

18029_wholemount_weak.wlz

18029_wholemount_possible.wlz

18029_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18029_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

homogeneous

weak expression: see section 01 02

humerus

weak

homogeneous

weak expression: see section 01 02

fibula

weak

homogeneous

weak expression: see section 01

tibia

weak

homogeneous

weak expression: see section 01

femur

weak

homogeneous

weak expression: see section 01

basioccipital bone

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 10 11 19 20 21 22

basisphenoid bone

weak

homogeneous

weak expression: see section 10 11 14

temporal bone petrous part

weak

homogeneous

weak expression: see section 01 02 21 22 23

orbito-sphenoid

weak

homogeneous

weak expression: see section 01 02 03 05 06 07 21

scapula

weak

homogeneous

weak expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37145
Entity Detected:	Tmc3, transmembrane channel-like gene family 3 ( MGI:2669033)
Sequence:	sense strand is shown >T37145 GTCTGTCATCGACATGCTCTTCACTGTGGCCAGCATCCTCCTCATAGACTTCTTCCGAGGACTTTTTGTT CGCTACCTAAGTGACTATTGGTGTTGGGACCTTGAAAGCAAGTTTCCTGAATATGGAGAATTCAAGATTG CCGAGAATGTGCTGCACCTTGTCTACAATCAAGGCATGATTTGGATGGGGGCCTTCTTCTCTCCGTGTCT GCCAGCATTCAATGTTCTTAAGCTCATTGGGCTGATGTACCTGAGGAGCTGGGCGGTGCTGACCTGCAAT GTACCCCACCAGCAAGTGTTTCGAGCTTCCAGGTCCAACAACTTCTACCTGGCAATGCTCCTGTTCATGT TGTTCCTTTGCATGCTGCCCACCATTTTTGCCATTGTCCACTACAAGCCGTCTCTGAACTGTGGTCCCTT CAGCGGACAAGAGAAAATTTATGACATCGTGTCCGAGACGATAGAGAATGACTTCCCGACCTGGTTTCAC GCTGTAGTTGGACATATCAGCAGCCCCGTGGTGATACTTCCAGCTGTGCTGCTTCTGTTCATGCTCATCT ACTACCTTCAGAGCATCGCGAGATCACTCAAGCTCAGCAGCCAGCAACTCAGGATGCAGATCCAAAATGC GAGATCTGAAGATAAGAAAAAGGTTGCCCAAATGGTAGAGGCCCTAGCCATCCCTAGCGATGCCAGGCAG GCAGGCTCAGCCACCGAAGCAGAGTCGAGTGAAAATTCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71517. Forward Primer - name:071517_F_cDNA_Tmc3, sequence:GTCTGTCATCGACATGCTCTTC; Reverse Primer - name:071517_N_SP6_cDNA_Tmc3, sequence:TGAATTTTCACTCGACTCTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18031 same embryo
	EMAGE:18030 same embryo
	EurExpress:euxassay_011796 same experiment
	MGI:4828753 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source