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EMAGE:18041

Tnfaip2 tumor necrosis factor, alpha-induced protein 2 ( MGI:104960)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041
"Pseudo-wholemount" of euxassay_011806. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011806_01 euxassay_011806_02 euxassay_011806_03 euxassay_011806_04
EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041
euxassay_011806_05 euxassay_011806_06 euxassay_011806_07 euxassay_011806_08 euxassay_011806_09
EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041
euxassay_011806_10 euxassay_011806_11 euxassay_011806_12 euxassay_011806_13 euxassay_011806_14
EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041 EMAGE:18041
euxassay_011806_15 euxassay_011806_16 euxassay_011806_17 euxassay_011806_18 euxassay_011806_19
EMAGE:18041
euxassay_011806_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18041Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18041_wholemount_strong.wlz
18041_wholemount_moderate.wlz
18041_wholemount_weak.wlz
18041_wholemount_possible.wlz
18041_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18041_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
saccule
moderate moderate
regionalmoderate expression: see section 19
cochlea
strong strong
regionalstrong expression: see section 01 moderate expression: see section 18 19
naris
weak weak
regionalweak expression: see section 12
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 07 08 09 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37154
Entity Detected:Tnfaip2, tumor necrosis factor, alpha-induced protein 2 ( MGI:104960)
Sequence:sense strand is shown

>T37154
GTCTGATATGCTAATGAGGCCCAGGGAACACCAGGCACCACACCAGTTGGGGATGCTCAGTGGCTCCAGC
TCCATGACCCATCATCAGGCTCCCACATTCTGCTGTTGCTTCTGCCCAGCCCTGGCTCTGGAACAAGATC
TGACAGCCAAGATCGCACAGATGATGGATCCAGTGGGGTCCAGACCTCACTGAAGGGAGCTGACATCTGA
GCATGCAAGAGATTTCTTGTAAATGATTATTCATCTTGGGTAGAGTGACCTTTGTCTACCTACACTGTAG
TCTGCCTTTTAACGGTCTGAGTTGCCCTGTCCAGCTCCCTAGGATCAGGGGTGAAGCCTCATTTATACCA
TTTCCTTCAGCCCAGGGATGTCATGAGATAGAACTAAAAACAAGGGAAGAAAGAAGAACTAGTATCTGAC
CCGGGGCCTGGAAGCAGGCCTGTGAAGTGCCCATGACAGAGCTAGACCCTGCAAAGTGTCCCCTGGTACC
ACTCAGCTAGCAGGACCCTCTTATTCACTCCCCATGGGCTGCCCTGCCCCTCTGTGTGTGTATGGATGTG
TTTGGGAAGTGACCCTGGAAGCCTTCGCAGAGCAGGGACCTCCCGGGTTCTCTCAGATCAAATCTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98724. Forward Primer - name:098724_F_cDNA_Tnfaip2, sequence:GTCTGATATGCTAATGAGGCCC; Reverse Primer - name:098724_N_SP6_cDNA_Tnfaip2, sequence:GAAGATTTGATCTGAGAGAACCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18039 same embryo
 EMAGE:18038 same embryo
 EMAGE:18040 same embryo
 EMAGE:18042 same embryo
 EurExpress:euxassay_011806 same experiment
 MGI:4828840 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS