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EMAGE:18138

Mdfi MyoD family inhibitor ( MGI:107687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138
"Pseudo-wholemount" of euxassay_011949. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011949_01 euxassay_011949_02 euxassay_011949_03 euxassay_011949_04
EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138
euxassay_011949_05 euxassay_011949_06 euxassay_011949_07 euxassay_011949_08 euxassay_011949_09
EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138
euxassay_011949_10 euxassay_011949_11 euxassay_011949_12 euxassay_011949_13 euxassay_011949_14
EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138
euxassay_011949_15 euxassay_011949_16 euxassay_011949_17 euxassay_011949_18 euxassay_011949_19
EMAGE:18138 EMAGE:18138 EMAGE:18138 EMAGE:18138
euxassay_011949_20 euxassay_011949_21 euxassay_011949_22 euxassay_011949_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18138Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18138_wholemount_strong.wlz
18138_wholemount_moderate.wlz
18138_wholemount_weak.wlz
18138_wholemount_possible.wlz
18138_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18138_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22
radius
moderate moderate
homogeneousmoderate expression: see section 23
humerus
moderate moderate
homogeneousmoderate expression: see section 01 02 03 18 19 20 21 22 23
fibula
moderate moderate
homogeneousmoderate expression: see section 01 02 23
tibia
moderate moderate
homogeneousmoderate expression: see section 01 02 23
femur
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 17 18 19 20 21 23
naris
moderate moderate
homogeneousmoderate expression: see section 08 09 12 13
nasal septum
moderate moderate
homogeneousmoderate expression: see section 10 11
viscerocranium
moderate moderate
homogeneousExpression in the turbinate bone.
meckel's cartilage
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 17 19 20 21
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14
basioccipital bone
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20
basisphenoid bone
moderate moderate
homogeneousmoderate expression: see section 10 11 12 13 14
temporal bone petrous part
moderate moderate
homogeneousmoderate expression: see section 01 02 18 19 20 21 22
vault of skull
moderate moderate
homogeneousmoderate expression: see section 01 02
orbito-sphenoid
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 16 17 18 19 20 21 22
scapula
moderate moderate
homogeneousmoderate expression: see section 02 03 19 20 21
pelvic girdle skeleton
moderate moderate
homogeneousmoderate expression: see section 07 08 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30012
Entity Detected:Mdfi, MyoD family inhibitor ( MGI:107687)
Sequence:sense strand is shown

>T30012
TGGGCTGGAGGTAGCAAGATCCACTCACCCTGTAGAGGCATCTTCTGAAGAGGGCTTCCCGGAGGAGGCG
GCACCCTCCATGCCCCATGACAGTGGTCTCCGGGCTCAGCAGGCTCTGAACAGCATTGACCTCGATGTCC
CCACAGAAGCTGTGACGTGCCAGCCTCAAGGGAACCCCCAAGGCTGCACCCCACTACTGCCAAATGGCTC
CAGCCACGACCACCTCTCAGAACCGGGCAGTGCAGGGCATGCGGGGAACGGTGCTCTGGGCGGGTCCAAG
GCCCACCGGAAGTTGCAGACGCATCCATCTCTGGGCAGCCAGGCTGGAAGGAAAAGCAGAGGCAGCGCCC
GGTCAGCCTCACAGGTCCCTCTCCAGGCACAGGAAGATTGCTGCGTCCACTGCATACTGTCCTGTCTATT
CTGTGAGTTCCTGACGCTCTGTAACATCCTCCTGGACTGCGCCACCTGTGGCTCCTGCAGCTCTGAGGAC
TCCTGCCTCTGCTGCTGCTGCTGTGGGTCCGGCGAGTGCGCGGACTGTGACCTGCCCTGCGACCTGGACT
GCGGCATCGTGGATGCCTGCTGCGAGTCCGCAGACTGCTTGGAGATATGCATGGAGTGCTGTGGACTCTG
TTTCTCCTCCTGATGCCATGGGGTGGCCCCAGAGCTACCGCACAAAGCTTGACGCCTCCCCTGACCCCGG
GCCGCCCCCTCAGAATCCCAACCCAGATGTGAGAAGGTGGGACGCTCAGAGGGGCCACCTCAGCCACCGA
ACAGGTCTGCTTTCAGACGCGTAGCCTGGTCCCCTCCACGGGTGACCAGGAACACGGCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3488088), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10791. Forward Primer - name:010791_F_IRAV12-15_E23_Mdfi, sequence:TGGGCTGGAGGTAGCAAG; Reverse Primer - name:010791_R_SP6_IRAV12-15_E23_Mdfi, sequence:GATGCCGTGTTCCTGGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18140 same embryo
 EMAGE:18135 same embryo
 EMAGE:18139 same embryo
 EMAGE:18137 same embryo
 EMAGE:18136 same embryo
 EurExpress:euxassay_011949 same experiment
 MGI:4826139 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS