EMAGE mouse gene expression spatial database

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EMAGE:18170

Ebi3 Epstein-Barr virus induced gene 3 ( MGI:1354171)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011954. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011954_01	euxassay_011954_02	euxassay_011954_03	euxassay_011954_04

euxassay_011954_05	euxassay_011954_06	euxassay_011954_07	euxassay_011954_08	euxassay_011954_09

euxassay_011954_10	euxassay_011954_11	euxassay_011954_12	euxassay_011954_13	euxassay_011954_14

euxassay_011954_15	euxassay_011954_16	euxassay_011954_17	euxassay_011954_18	euxassay_011954_19

euxassay_011954_20	euxassay_011954_21	euxassay_011954_22	euxassay_011954_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18170_wholemount_strong.wlz

18170_wholemount_moderate.wlz

18170_wholemount_weak.wlz

18170_wholemount_possible.wlz

18170_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18170_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 04 18 19 20 21 22 23

fibula

moderate

homogeneous

moderate expression: see section 01

tibia

moderate

homogeneous

moderate expression: see section 01 02 03

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21 22 23

otic capsule

moderate

homogeneous

moderate expression: see section 08 09 10 15 16

naris

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17

nasal septum

moderate

homogeneous

moderate expression: see section 14

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

hyoid

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16

meckel's cartilage

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

axial skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

basioccipital bone

moderate

homogeneous

moderate expression: see section 03 04 05 17 18 19 20 21 22 23

basisphenoid bone

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 03 04 05 06 17 18 19 20 21 22 23

vault of skull

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 22 23

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

scapula

moderate

homogeneous

moderate expression: see section 02 19 20

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 07 08 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30042
Entity Detected:	Ebi3, Epstein-Barr virus induced gene 3 ( MGI:1354171)
Sequence:	sense strand is shown >T30042 CCCTGTGTGAGTCCCCTGTCCTTCACCCTCTCTCTGATGGGTCACTAACTCGGATCCAAGGAACAGAGCC ACAGAGCATGTCCAAGCTGCTCTTCCTGTCACTTGCCCTCTGGGCCAGCCGCTCCCCTGGTTACACTGAA ACAGCTCTCGTGGCTCTAAGCCAGCCCAGAGTGCAATGCCATGCTTCTCGGTATCCCGTGGCCGTGGACT GCTCCTGGACTCCTCTCCAGGCTCCCAACTCCACCAGATCCACGTCCTTCATTGCCACTTACAGGCTCGG TGTGGCCACCCAGCAGCAGAGCCAGCCCTGCCTACAACGGAGCCCCCAGGCCTCCCGATGCACCATCCCC GACGTGCACCTGTTCTCCACGGTGCCCTACATGCTAAATGTCACTGCAGTGCACCCAGGCGGCGCCAGCA GCAGCCTCCTAGCCTTTGTGGCTGAGCGAATCATCAAGCCGGACCCTCCGGAAGGCGTGCGCCTGCGCAC AGCGGGACAGCGCCTGCAGGTGCTCTGGCATCCCCCTGCTTCCTGGCCCTTCCCGGACATCTTCTCTCTC AAGTACCGACTCCGCTACCGGCGCCGAGGAGCCTCTCACTTCCGCCAGGTGGGACCCATTGAAGCCACGA CTTTCACCCTCAGGAACTCGAAACCCCATGCCAAGTATTGCATCCAGGTGTCAGCTCAGGACCTCACAGA TTATGGGAAACCAAGTGACTGGAGCCTCCCTGGGCAAGTAGAAAGTGCACCCCATAAGCCCTGAAGAGGG ACTGGATCCTTGATATCAGACCCTGATGTCGTCTACTTGAGGGTGGCAGGGTGGCCTGTCCTGAGTCTGA ATACTGACTTTCCATGTACTGGGCTGCTCCGAAGCACTGGATAATTCACTTGACTTCTTCAGACCTCAAT TTCCAACCCTGTGGGATGATCTTTCTTCTTCTGCCGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3154957), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15190. Forward Primer - name:015190_F_IRAV16-19_E01_Ebi3, sequence:CCCTGTGTGAGTCCCCTG; Reverse Primer - name:015190_R_SP6_IRAV16-19_E01_Ebi3, sequence:GCACCGGCAGAAGAAGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18172 same embryo
	EMAGE:18171 same embryo
	EMAGE:18173 same embryo
	EMAGE:18169 same embryo
	EurExpress:euxassay_011954 same experiment
	MGI:4824459 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source