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EMAGE:18223

Tnfaip6 tumor necrosis factor alpha induced protein 6 ( MGI:1195266)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18223 EMAGE:18223 EMAGE:18223 EMAGE:18223 EMAGE:18223
euxassay_012017_01 euxassay_012017_02 euxassay_012017_03 euxassay_012017_04 euxassay_012017_05
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euxassay_012017_06 euxassay_012017_07 euxassay_012017_08 euxassay_012017_09 euxassay_012017_10
EMAGE:18223 EMAGE:18223 EMAGE:18223 EMAGE:18223 EMAGE:18223
euxassay_012017_11 euxassay_012017_12 euxassay_012017_13 euxassay_012017_14 euxassay_012017_15
EMAGE:18223 EMAGE:18223 EMAGE:18223 EMAGE:18223 EMAGE:18223
euxassay_012017_16 euxassay_012017_17 euxassay_012017_18 euxassay_012017_19 euxassay_012017_20
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euxassay_012017_21 euxassay_012017_22 euxassay_012017_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18223Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18223_wholemount_strong.wlz
18223_wholemount_moderate.wlz
18223_wholemount_weak.wlz
18223_wholemount_possible.wlz
18223_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18223_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30090
Entity Detected:Tnfaip6, tumor necrosis factor alpha induced protein 6 ( MGI:1195266)
Sequence:sense strand is shown

>T30090
ATGGAATCCGGCTCAACAGGAGTGAGCGGTGGGATGCCTATTGCTACAACCCACACGCAAAGGAGTGTGG
TGGTGTCTTCACAGATCCGAAGCGAATTTTTAAATCCCCGGGCTTCCCAAATGAGTACGATGACAACCAG
GTCTGCTACTGGCACATTCGGCTCAAGTACGGTCAGCGAATTCACCTGAGCTTTTTGGACTTTGACCTTG
AACATGATCCAGGCTGCTTGGCTGACTATGTAGAAATCTATGACAGTTATGATGACGTCCACGGCTTTGT
AGGAAGATACTGTGGTGATGAACTTCCAGAAGACATCATTAGCACAGGAAATGTCATGACCTTGAAGTTT
CTGAGTGATGCATCCGTCACGGCTGGAGGCTTCCAGATTAAATACGTCACAGTGGATCCTGCATCTAAAT
CCAGTCAAGCCAAAAATACAAGTACTACTGGAAATAAGAAGTTCTTACCTGGAAGGTTTAGCCATCTATA
AAAAAAATTTTTAAAAAATGTTCAAAACATCCAGTACAATATTTATATTTGTTTTTGTTGTTGTTGTTGG
GTTTTGTTTTGTTTTGTTTTGTTTTTTCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTC
ACTTTGAAGATCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTCCGCCTACCAAGTGCTGGGATTAAA
GGCGTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4208738), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 38282. Forward Primer - name:038282_F_IRAV20-23_L04_Tnfaip6, sequence:ATGGAATCCGGCTCAACA; Reverse Primer - name:038282_R_SP6_IRAV20-23_L04_Tnfaip6, sequence:TGGACGCCTTTAATCCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18225 same embryo
 EMAGE:18222 same embryo
 EMAGE:18221 same embryo
 EMAGE:18224 same embryo
 EurExpress:euxassay_012017 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS